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TaKaRa
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Á¦Ç°¸í/Á¦Ç°ÄÚµå
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Cloned

DNA Polymerase I (E. coli )

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2130A
DNA Polymerase I£¨E. coli£©
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
500 U
74,000¿ø  2130A_DS.v1901Da.pdf
Takara
2130B
DNA Polymerase I£¨E. coli£©
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
2,500 U
333,000¿ø 

³óµµ 3~6 U/§¡
Çü»ó
50 mM Potassium Phosphate (pH6.5)
10 mM 2-Mercaptoethanol
50% Glycerol
º¸Á¸ -20¡É
÷ºÎ Buffer Á¶¼º (10¡¿)
500 mM Tris-HCl (pH7.8)
100 mM MgCl2
1 mM DTT
250 §¶/ml BSA*
*BSA°¡ ħÀüµÉ ¿ì·Á°¡ ÀÖÀ¸¹Ç·Î µ¿°á¡¤ À¶ÇØ´Â °¡´ÉÇÑ ÇÇÇÑ´Ù.
±â¿ø
Escherichia coli carrying the plasmid which encodes the gene of Pol I
Á¦Ç°¼³¸í
º» È¿¼Ò´Â ÁÖÇü, ÇÁ¶óÀ̸Ó(DNA, RNA ¸ðµÎ °¡´É) Á¸ÀçÇÏ¿¡¼­ dNTP¸¦ ±âÁú·Î ÇÏ¿©, ÁÖÇü¿¡ »óº¸ÀûÀÎ DNA¸¦ 5¡¯¡æ 3¡¯¹æÇâÀ¸·Î ÇÕ¼ºÇÑ´Ù. ÀÌÁß°¡´Ú ƯÀÌÀû 5¡¯¡æ3¡¯exonuclease Ȱ¼º ¹× ´ÜÀϰ¡´Ú ƯÀÌÀû 3¡¯¡æ 5¡¯exonuclease Ȱ¼ºµµ °¡Áø´Ù.
Ȱ¼ºÀÇ Á¤ÀÇ
Poly d(A-T)ÇÕ¼º DNA¸¦ ÁÖÇü/ÇÁ¶óÀ̸ӷΠÇÏ¿© 37¡É, pH7.4¿¡¼­, 30ºÐ µ¿¾È ¸ðµç 10 nmol nucleotide¸¦ »êºÒ¿ë¼º ħÀü¹°·Î ¹Ù²Ù´Â È¿¼Ò Ȱ¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
Ȱ¼ºÃøÁ¤¿ë ¹ÝÀÀ¾×Á¶¼º
67 mM Potassium phosphate (pH7.4)
6.7 mM MgCl2
1 mM 2-Mercaptoethanol
20 ¥ìM ±âÁú DNA
33 ¥ìM dATP
33 ¥ìM [3H]dTTP
¼øµµ
- SDS Àü±â¿µµ¿¿¡¼­ 90% ÀÌ»óÀÇ ¼øµµ¸¦ ³ªÅ¸³½´Ù.
- 10 UÀÇ º» È¿¼Ò¿Í 1§¶ÀÇ closed circular (RF I)¬æX174 DNA¸¦ 37¡É, 1½Ã°£ ¹ÝÀÀÇÏ¿©µµ DNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È­°¡ ÀϾÁö ¾Ê´Â´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
- »ó±â º¸Á¸Á¶°Ç¿¡¼­ 6°³¿ù ÀÌ»ó ¾ÈÁ¤Çϰí Èñ¼®¿¡ ÀÇÇÑ È°¼ºÀúÇÏ´Â ÀϾÁö ¾ÊÀ¸³ª, ½ÉÇÏ°Ô ±³¹ÝÇÏ¸é ½ÇȰÇÒ ¼öµµ ÀÖ´Ù.
- Endonuclease¸¦ Æ÷ÇÔÇϰí ÀÖÁö ¾ÊÀ¸¹Ç·Î ´Üµ¶À¸·Î´Â °ÅÀÇ nick translation Ȱ¼ºÀº ³ªÅ¸³»Áö ¾Ê´Â´Ù.
- DNA¿Í ģȭ¼ºÀÌ °­Çϱ⠶§¹®¿¡ °ú·®À» »ç¿ëÇϸé aggregationÀÌ ÀϾ ¹ÝÀÀÀÌ ÃæºÐÈ÷ ÁøÇàµÇÁö ¾Ê´Â °æ¿ì°¡ ÀÖ´Ù.
¿ëµµ
- DNase I (TaKaRa Code 2210)°úÀÇ º´¿ë¿¡ ÀÇÇÑ nick translation
- Okayama-Berg¹ý¿¡ À־ second strandÀÇ ÇÕ¼º¹ÝÀÀ (DNA polymerase I 0.3 §¶Àº ¾à 2.5 U¿¡ »ó´çÇÑ´Ù).

Keyword :
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¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢RNA Polymerase
Cloned
> DNA Polymerase I (E. coli )
Cloned
> Klenow Fragment (Large Fragment E. coli DNA Polymerase I)
> Poly(A) Polymerase
Cloned
> SP6 RNA Polymerase
Cloned
> T4 DNA Polymerase
Cloned
> T7 RNA Polymerase
Cloned
> Terminal Deoxynucleotidyl Transferase
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