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Plant Genomic DNA Extraction Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9768A
TaKaRa MiniBEST Plant Genomic DNA Extraction Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
50 ȸ
(Package)
153,600¿ø 
192,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9768_e.v1309Da.pdf
Takara
9768B
TaKaRa MiniBEST Plant Genomic DNA Extraction Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9768A x 4)
692,000¿ø 


»ó¼¼Á¤º¸
TaKaRa MiniBEST Plant Genomic DNA Extraction Kit´Â ½Ä¹°Á¶Á÷À¸·ÎºÎÅÍ genomic DNA¸¦ Á¤Á¦ÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº µÎ °¡ÁöÀÇ ÇÁ·ÎÅäÄÝ (ÀϹÝÀûÀÎ ½Ä¹°Á¶Á÷¿¡¼­ genomic DNA¸¦ ÃßÃâÇÏ´Â ¹æ¹ý°ú polysaccharide³ª polyphenolÀ» ´Ù·® ÇÔÀ¯ÇÑ ½Ä¹°Á¶Á÷À¸·ÎºÎÅÍ genomic DNA¸¦ ÃßÃâÇÏ´Â ¹æ¹ý)À» Àû¿ëÇÏ°í ÀÖ´Ù.
º» Á¦Ç°À» »ç¿ëÇÏ¸é ¾×üÁú¼Ò¸¦ ÀÌ¿ëÇÑ ½Ä¹°Á¶Á÷ grinding ÀÌÈÄ, 40ºÐ ³»¿¡ °í¼øµµÀÇ genomic DNA¸¦ ÃßÃâ °¡´ÉÇÏ´Ù. (50 - 500 mgÀÇ ½Ä¹° Á¶Á÷À¸·Î ºÎÅÍ 1 - 10ugÀÇ °í¼øµµ genomic DNA¸¦ ¾òÀ» ¼ö ÀÖ´Ù.) Á¤Á¦ÇÑ genomic DNA´Â PCR, Southern blotting, RAPD, AFLP, RFLP¿Í °°Àº ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë°¡´ÉÇÏ´Ù.
Flowchart
³»¿ë
¢º Part I
50X DTT Buffer

700 ul

RNaseA

500 ul



¢º Part II
Buffer HS I*1,3

28 ml

Buffer HS II*1

28 ml

Buffer KAC

1.8 ml x 2

Buffer GB*1

28 ml

Buffer WA*1

28 ml

Buffer WB*2

24 ml

Elution Buffer

14 ml

Spin Columns

50

Collection tubes

50


*1: Contains strong denaturant. Be careful to avoid contacting with skin and eyes. In the case of such contact, wash immediately with plenty
     water and seek medical advice.
*2: Before using the kit, add 56 ml of 100% ethanol. Mix well.
*3: When Buffer HS I form precipitate by storage bellow room temperature (15 - 25¡É ), redissolve the precipitate by incubating at 37¡É before
     used.

¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% ethanol
2. Sterile distilled water
º¸°ü ¹× ¿î¼Û
1. Part I can be stored at -20¡É, and Part II can be stored at room temperature (15 - 25¡É ).
2. Part I can be shipped at -20¡É, and Part II can be shipped at room temperature (15 - 25¡É ).

Keyword : ½Ä¹° DNA,½Ä¹° gDNA

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