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Agarose Gel DNA Extraction Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9762A
TaKaRa MiniBEST Agarose Gel DNA Extraction Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
79,200¿ø 
99,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9762_e.v1306Da.pdf
Takara
9762B
TaKaRa MiniBEST Agarose Gel DNA Extraction Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9762A x 4)
356,000¿ø 


»ó¼¼Á¤º¸
TaKaRa MiniBEST Agarose Gel DNA Extraction KitÀº agarose gel¿¡¼­ DNA ´ÜÆíÀ» ½Å¼ÓÇÏ°Ô Á¤Á¦Çϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù. º» Kit´Â agarose gelÀ» ¿ëÇؽÃÅ°´Â Àü¸Å buffer¿Í DNA fragment¸¦ Á¤Á¦Çϱâ À§ÇÑ columnÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù. Buffer´Â °­ÇÑ ¹öÆÛ Æ¯Â¡À» °®°í ÀÖÀ¸¸ç Spin column membrane¿¡ ÃÖÀûÈ­µÈ pH°ªÀ» °®´ÂÁö ½±°Ô È®ÀÎÇÒ ¼ö ÀÖ´Â pH indicator¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. Ãß°¡·Î buffer´Â °¡¿­¾øÀÌ ½Ç¿Â(15~25¡É)¿¡¼­ gelÀ» ºü¸£°Ô ³ìÀÏ ¼ö ÀÖ´Ù. 20ºÐÀ̳» ½ÇÇè Àü °úÁ¤À» ¼öÇàÇÒ ¼ö ÀÖ´Ù. Spin ColumnÀº ÃÖ´ë 20 ¥ìg±îÁö DNA¸¦ °áÇÕ½Ãų ¼ö ÀÖ°í 50 bp~20 kbpÀ̳»ÀÇ DNA ´ÜÆíÀ» È¿°úÀûÀ¸·Î ȸ¼öÇÒ ¼ö ÀÖ´Ù. ȸ¼öÀ²Àº ¾à 50~80% Á¤µµµÈ´Ù. ±×·¯³ª DNA ´ÜÆíÀÌ 20~50 kbp»çÀ̸é ȸ¼öÀ²Àº ´õ ¶³¾îÁú ¼ö ÀÖ´Ù. º» kitÀ¸·Î Á¤Á¦ÇÑ DNA´Â ligation, PCR, sequencing°ú °°Àº ¸ðµç Á¾·ùÀÇ ºÐÀÚ»ý¹°ÇÐ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.

³»¿ë
1¹ÝÀÀ ´ç 300 mgÀÇ agarose gel(1% ³óµµÀÇ agarose ±âÁØ)À» ó¸®ÇÒ ¼ö ÀÖ´Ù.
º» KitÀº Reagent Set¿Í Column SetÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
Reagent Set

Buffer GM£ª1

50 ml

Buffer WB£ª2

24 ml

Elution Buffer

2ml x 2


£ª1: Contain strong denaturant. Be careful to avoid contacting with eyes and skin. In the case of such contact, wash immediately with plenty
      water and seek medical advice.
£ª2: Before the first use of the kit, add 56 ml of 100% ethanol to Buffer WB.
Column Set

Spin Columns

50

Collection tubes

50


¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% Ethanol
2. Sterilized water or Tris-HCl (pH8.0)
3. 3 M sodium acetate (pH5.2)
º¸°ü ¹× ¿î¼Û
1. The kit can be stored at room temperature (15 - 25¡É).
2. The kit is shipped at room temperature (15 - 25¡É).
3. Precipitate may occur in the Buffer GM under low temperature. Please warm with a 37¡É to dissolve and use after returning to room
    temperature.

Keyword :

Agarose Gel·Î ºÎÅÍ ÇÙ»ê ȸ¼ö
>
³î¶ó¿ï Á¤µµ·Î °£Æí, ½Å¼ÓÇÑ DNA ´ÜÆí ȸ¼ö ½Ã..
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