
* 9¿ù ÇÑ´Þ °£ »ùÇÃÀ» »ç¿ë ÇØ º¸½Ã°í ¡°
»ùÇà »ç¿ë Èı⡱¸¦ °í°´Áö¿ø ¸ÞÀÏ(
support@takara.co.kr)·Î º¸³» Áֽøé Ä¿ÇÇ¿Í ÇÇÀÚ¸¦ ¼±¹°·Î µå¸³´Ï´Ù.
-
À̺¥Æ® °øÁö»çÇ× º¸·¯°¡±â~~
- Æ¯ÈµÈ Lysis buffer »ç¿ë : ÇϳªÀÇ lysis buffer·Î ´Ù¾çÇÑ µ¿/½Ä¹° Á¶Á÷ ¹× ¼¼Æ÷, bacteria¿¡¼ total RNA ÃßÃâ °¡´É
- °£ÆíÇÑ gDNA Á¦°Å: º°µµÀÇ DNase I 󸮰úÁ¤ ¾øÀÌ gDNA Eraser Spin ColumnÀ» »ç¿ëÇÏ¿© gDNA Á¦°Å
- ºü¸£°í °£ÆíÇÑ RNA ÃßÃâ : ¼¼Æ÷ ÆÄ¼â ÈĺÎÅÍ RNA ÃßÃâ±îÁö 20ºÐ ¼Ò¿ä
- ´Ù¾çÇÑ »ùÇÃÀÇ RNAÃßÃâÀ» À§ÇÑ Protocol È®ÀÎ : ¹ÚÅ׸®¾Æ, Ç÷¾×, È¿¸ð
»ó¼¼Á¤º¸
TaKaRa MiniBEST Universal RNA Extraction Kit´Â ´Ù¾çÇÑ µ¿/½Ä¹° ¼¼Æ÷ ¶Ç´Â Á¶Á÷À¸·ÎºÎÅÍ °í¼øµµ total RNA¸¦ ½Å¼ÓÇÏ°Ô ÃßÃâÇϰíÀÚ ÇÒ ¶§ ÀûÇÕÇÑ Á¦Ç°ÀÌ´Ù.
º» Á¦Ç°Àº ½Å¼ÓÇϰí, Æí¸®ÇÏ°Ô total RNA¸¦ ÃßÃâÇϱâ À§ÇØ ¼¼Æ÷³ª Á¶Á÷À» ¾×üÁú¼Ò·Î ºÐ¼âÇϰųª ±ÕÁúÈ (homogenization) ÈÄ phenol°ú chloroformÀ» »ç¿ëÇÏÁö ¾Ê°í,
Æ¯ÈµÈ lysis bufferÀ» »ç¿ëÇÏ¿© ¿ëÇØ½ÃŲ´Ù. ¶ÇÇÑ ¿ø½ÉºÐ¸®·Î genome DNA¸¦ Á¦°ÅÇÏ´Â gDNA Eraser Spin Column°ú RNA°¡ °áÇÕÇÏ´Â RNA Spin ColumnÀ¸·Î ±¸¼ºµÇ¾î ÀÖ¾î
°í¼øµµ, °íǰÁú RNA Á¤Á¦°¡ °¡´ÉÇÏ´Ù. ¿ëÇØµÈ Á¶Á÷À̳ª ¼¼Æ÷·ÎºÎÅÍ 20ºÐÀ̳» °í¼øµµ total RNA¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°À¸·Î ÃßÃâÇÑ RNA´Â ´Ü¹éÁúÀ̳ª genomic DNA¸¦ °ÅÀÇ Æ÷ÇÔÇÏÁö ¾ÊÀ¸¸ç 105~107 ¹è¾ç¼¼Æ÷, 5~40 mg µ¿¹°Á¶Á÷À̳ª 5~100 mg ½Ä¹° Á¶Á÷À¸·ÎºÎÅÍ ÃÖ´ë ¼ö½Ê §¶ÀÇ RNA¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
Á¤Á¦µÈ °íǰÁú RNA´Â Northern blot, dot blot, mRNA purification, in vitro translation, RNase protection assay, RT-PCR, Real Time RT-PCR,
cDNA library ±¸ÃàÀ̳ª ±× ¿Ü ´Ù¾çÇÑ ºÐÀÚÀ¯ÀüÇÐ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù

±×¸² 1. TaKaRa MiniBEST Universal RNA Extraction KitÀÇ workflow

±×¸² 2. ¹è¾ç ¼¼Æ÷¿¡¼ total RNA ÃßÃâ. TaKaRa MiniBEST Universal RNA Extraction KitÀ» »ç¿ëÇÏ¿© 1.0 x 106ÀÇ HL60 cell¿¡¼
10 §¶ÀÇ total RNA¸¦ ÃßÃâÇÏ¿´´Ù. Àü±â¿µµ¿ °á°ú °í¼øµµÀÇ RNA°¡ ÃßÃâµÈ °ÍÀ» È®ÀÎÇÏ¿´´Ù.

±×¸² 3. µ¿¹° Á¶Á÷¿¡¼ total RNA ÃßÃâ. TaKaRa MiniBEST Universal RNA Extraction KitÀ» »ç¿ëÇÏ¿© 5 - 10 §·ÀÇ ´Ù¾çÇÑ ¸¶¿ì½º Á¶Á÷¿¡¼ total RNA¸¦ ÃßÃâÇÏ¿´´Ù.
ÃßÃâµÈ RNAÀÇ Àü±â¿µµ¿ °á°ú, °í¼øµµÀÇ RNA°¡ ÃßÃâµÈ °ÍÀ» È®ÀÎÇÏ¿´´Ù

±×¸² 4. ½Ä¹° Á¶Á÷¿¡¼ total RNA ÃßÃâ. TaKaRa MiniBEST Universal RNA Extraction KitÀ» »ç¿ëÇÏ¿© 50 §·ÀÇ ¹öµå³ª¹«¿Í ¿Á¼ö¼ö ÀÙ¿¡¼ total RNA¸¦ ÃßÃâÇÏ¿´´Ù.
ÃßÃâµÈ RNAÀÇ Àü±â¿µµ¿ °á°ú, °í¼øµµÀÇ RNA°¡ ÃßÃâµÈ °ÍÀ» È®ÀÎÇÏ¿´´Ù
³»¿ë
º» Kit´Â Part I°ú Part II·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
¢ºPart I
50X DTT Solution |
700 §¡ |
Recombinant DNase I (RNase-free£»5 U/§¡) |
1000 U |
10X DNase I Buffer |
1 §¢ |
¢ºPart II
Buffer RL |
32 §¢ |
Buffer RWA |
28 §¢ |
Buffer RWB |
30 §¢ |
RNase Free dH2O |
15 §¢ |
gDNA Eraser Spin Column |
50 ea |
RNA Spin Column |
50 ea |
Collection Tube (2 §¢) |
50 ea |
RNase Free Collection tube (1.5 §¢) |
50 ea |
º¸Á¸
1£® Part I : -20¡É
2£® Part II : ½Ç¿Â