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Plasmid Purification Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9760A
TaKaRa MiniBEST Plasmid Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
76,000¿ø 
95,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9760_e.v1706Da.pdf
Takara
9760B
TaKaRa MiniBEST Plasmid Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9760A x 4)
344,000¿ø 


»ó¼¼Á¤º¸
TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0Àº ¼Ò·®ÀÇ ¹ÚÅ׸®¾Æ ¹è¾ç¾×À¸·ÎºÎÅÍ plasmid DNA¸¦ ½Å¼ÓÇÏ°Ô Á¤Á¦ÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÈ Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº ¹ÚÅ׸®¾Æ ¼¼Æ÷¸¦ ¿ëÇØÇÏ´Â ÀüÅëÀûÀÎ SDS-alkaline lysis ¹æ¹ýÀ» ±â¹ÝÀ¸·Î ÇÏ¿© plasmid DNA°¡ ¼±ÅÃÀûÀ¸·Î Spin columnÀÇ silica membrane¿¡ ºÎÂøÇÏ´Â ¹æ¹ýÀÌ °áÇÕµÈ Á¦Ç°À¸·Î Ưº°ÇÑ buffer¿¡ ÀÇÇØ È°¼ºÈ­µÈ´Ù. ÇÁ·ÎÅäÄÝÀº °í¼øµµ plasmid DNA¸¦ ½Å¼ÓÇÏ°Ô Á¤Á¦ÇÏ´Â °£ÆíÇÑ ¹æ¹ýÀ¸·Î 1½Ã°£ À̳» Àüü °úÁ¤À» ¿Ï·áÇÒ ¼ö ÀÖ´Ù.
LB ¹èÁö¿¡ ¹ã»õ ¹è¾çÇÑ 1~4 mlÀÇ ¹ÚÅ׸®¾Æ ¹è¾ç¾×À¸·ÎºÎÅÍ 1~20 ugÀÇ ¼ø¼öÇÑ plasmid DNA (OD260/OD280 = 1.8¢¦2.0)¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. Æä³î ÃßÃâ°ú ¿¡Åº¿Ã ħÀü °úÁ¤ÀÌ ÇÊ¿ä ¾ø´Â ÇÁ·ÎÅäÄÝÀ̸ç Tris ¹öÆÛ³ª Á¤Á¦¼ö¿¡ ³ìÀÎ plasmid DNA´Â DNA sequencingÀ̳ª transformation, in vitro transcription, Á¦ÇÑÈ¿¼Ò 󸮳ª ±× ¿Ü È¿¼Ò ¹ÝÀÀÀÌ ÇÊ¿äÇÑ ´Ù¾çÇÑ ½ÇÇè¿¡ Á÷Á¢ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë
Reagent Set

RNase A (10 mg/ml)

140 ¥ìl

Solution l

14 ml

Solution ll£ª1

14 ml

Solution lll£ª2

24 ml

Buffer WA£ª2

28 ml

Buffer WB£ª3

24 ml

Elution Buffer

2 ml x 2


£ª1: Contain alkali solution. Be careful to avoid contacting with skin and eyes. In the case of such contact, rinse immediately with plenty of
      water and seek medical advice.
£ª2: Contain strong denaturant. Be careful to avoid contacting with skin and eyes. In the case of such contact, rinse immediately with plenty of
      water and seek medical advice.
£ª3: Before the first use of the kit, add 56 ml of 100% ethanol to Buffer WB.
Column Set

Spin Columns

50

Collection tubes

50


¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% ethanol
2. Sterilized water or Tris-HCl (pH8.0)
º¸°ü ¹× ¿î¼Û
  • 1. The kit can be stored at room temperature (15 - 25¡É) for 1 year.
        The kit should be stored at 4¡É for long-term preservation.
        If a precipitate is visible in some buffer, incubate the buffer at 37¡É for several minutes until the precipitate is dissolved completely.
  • 2. RNase A can be stored at room temperature (15 - 25¡É) stable for 6 months.
        It should be stored at - 20¡É for long-term storage.
  • 3. After adding RNase A to Solution l, it can be stored at 4¡É for 3 months.
  • 4. The kit is shipped at room temperature (15 - 25¡É).

Keyword : cloning,Ŭ·Î´×,e.coli,vectorÁ¤Á¦,plasmid prep,mini prep,miniprep

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