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Home > 전제품보기 > NGS 관련 > Ultra Low/Single cell-Seq > SMARTer Ultra Low RNA Kit for the Fluidigm C1 System

SMARTer Ultra Low RNA Kit for the Fluidigm C1 System

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제조사 제품코드 제품명 용량 가격
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비고 사용자매뉴얼
Clontech
634832
SMARTer Ultra Low RNA Kit for the Fluidigm® C1™ System, 2 IFCs
관련학술 관심상품등록 구매하기 라이선스 
Each
1,923,000원  제조사 페이지로 바로가기 C1_mRNA_Seq_pr_100_7168.pdf
Clontech
634833
SMARTer Ultra® Low RNA Kit for the Fluidigm® C1™ System, 10 IFCs
관련학술 관심상품등록 구매하기 라이선스 
Each
6,957,000원  제조사 페이지로 바로가기 C1_mRNA_Seq_pr_100_7168.pdf
Clontech
635025
SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 4 IFCs
관련학술 관심상품등록 구매하기 라이선스 
Each
2,461,000원  제조사 페이지로 바로가기 116809
Clontech
635026
SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 10 IFCs
관련학술 관심상품등록 구매하기 라이선스 
Each
4,922,000원  제조사 페이지로 바로가기 116809

"SMART-Seq Ultra Low Input: Single cell sequencing (단일세포 염기서열 분석) 논문리스트 보기"
[Fluidigm® Script Hub] Fluidigm® C1™을 이용한 Full length mRNA Sequencing에 최적

  • High-quality single-cell RNA-seq data
  • NGS Library construction in 2 days
제품 설명
SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System
Our latest single-cell RNA-seq kit is the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System, IFCs. This kit allows high-quality cDNA synthesis from IFC-captured cells (96-IFC format) processed with the Fluidigm C1 Single-Cell Auto Prep IFC for mRNA Seq or Open App. Its method improves upon the previous generations by incorporating the novel use of locked nucleic acid (LNA) technology into an optimized template-switching oligo (our proven SMART technology), and other advancements developed by Clontech scientists. The new version contains SeqAmp polymerase, which excels even in GC- and AT-rich regions and promotes better transcript representation.

SMART-Seq v4 kits, including the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System, IFCs, produce single-cell mRNA-seq libraries that clearly outperform previously-published protocols and older products. SMART-Seq v4 kits provide higher sensitivity and reproducibility (meaning more genes are identified from libraries produced with these kits) and exhibit significantly lower background than the other methods.
특징
  • High-quality single-cell RNA-seq data - whole transcriptome full-length mRNA-seq information, better representation of GC-rich genes, and good reproducibility with reduced technical variation.
  • Library construction in 2 days - hands-on aspects of the cDNA synthesis protocol can be completed in approximately two hours, followed by running the eight-hour SMART-Seq v4 script on the C1 instrument. The entire library construction protocol can be completed within two working days.
  • New script available online - the script for using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System is available in the Fluidigm Script Hub. The user manual for this product is available on the Documents tab of this web page.
Interested in more data and FAQs about this product? Visit the NGS Resource Portal.
Applications
  • cDNA synthesis for mRNA-seq from up to 96 single cells on the Fluidigm C1 System
  • cDNA synthesis for qPCR analysis on the Fluidigm Biomark platform prior to Illumina sequencing
구성품 (자세한 내용은 CoA를 참조하세요)
  • SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 4 IFCs Components
  • SeqAmp™ DNA Polymerase

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