High-Purity His-Tagged Protein Purification with TALON® Resin

TALON His Tag Purification Resin lets you prepare exceptionally pure his-tagged proteins from bacterial, mammalian, yeast, and baculovirus-infected cells, under native or denaturing conditions. TALON is an immobilized metal affinity chromatography (IMAC) resin charged with cobalt, which binds to his-tagged proteins with higher specificity than nickel-charged resins. As a result, TALON resin delivers his-tagged proteins of the highest purity. In addition, each cobalt ion is bound to the resin at four sites, resulting in low metal ion leakage. TALON resin is available in a variety of formats. Use the following tables to decide which one is best for your specific application.

	Prepacked Columns
	TALON Single Step	HisTALON™ Superflow Cartridge	HisTALON Gravity Columns	TALON Spin Columns
Prepacked columns	Yes	Yes	Yes	Yes
Binding capacity (mg protein/ml resin)	5–10	5–20	5–20	4–8
Starting material	Bacterial culture	Clarified lysate	Clarified lysate	Clarified lysate
FPLC applications	No	Yes	No	No
Time savings	+++++	+++	+++	+++++
Maximum pressure	N/A	75 psi 5 bar 0.5 MPa	N/A	N/A
Maximum flow rate (ml/min)	N/A	10 (1 ml cartridge) 40 (5 ml cartridge)	N/A	N/A
Bead size (µm)	300–500	60–160	45–165	45–165
Downstream applications	Preliminary characterization of target proteins Fast capture of his-tagged proteins In-column protein extraction	X-ray crystallography Functional assays Structural investigations	X-ray crystallography Functional assays Structural investigations	Simultaneously purify several proteins Verify his-tagged protein expression in transformants Optimize purification protocols

	Purification Resins
	TALON Metal Affinity Resin	TALON Superflow Metal Affinity Resin	TALON Magnetic Beads	TALON CellThru
Prepacked columns	No	No	No	No
Binding capacity (mg protein/ml resin)	5–15	5–18	2–15	5–10
Starting material	Clarified lysate	Clarified lysate	Clarified lysate	Unclarified lysate
FPLC applications	No	Yes	No	No
Time savings	+	+	+++	+++
Maximum pressure	2.8 psi 0.25 bar 0.02 MPa	150 psi 10 bar 0.97 MPa	N/A	9 psi 0.62 bar 0.06 MPa
Maximum flow rate (ml/min)	0.5	50	N/A	13
Bead size (µm)	45–165	60–160	20–75	300–500
Downstream applications	X-ray crystallography Functional assays Structural investigations	X-ray crystallography Functional assays Structural investigations	Microscale protein purification Scaleable purification protocols Simultaneously purify several proteins Verify his-tagged protein expression in transformants	X-ray crystallography Functional assays Structural investigations Purify proteins from crude cell lysates, sonicates & fermentor harvests