Use the list below to learn about kits to improve your CRISPR/Cas9 workflow.
Use the Guide-it sgRNA In Vitro Transcription Kit to produce high yields of single guide RNAs (sgRNAs) for Cas9/CRISPR studies. The kit includes the Guide-it IVT RNA Clean-Up Kit, a phenol-free kit used to purify sgRNA following in vitro transcription (IVT) reactions. The purified sgRNA is ready for use in transfection, electroporation, or in vitro cleavage assays.
Guide-it sgRNA In Vitro Transcription Kit workflow.
All necessary reagents are included in the kit:
Avoid delivering ineffective single guide RNAs (sgRNAs) to your cells—use the Guide-it sgRNA Screening Kit to test the efficacy of sgRNAs.
Guide-it sgRNA Screening Kit workflow.
All necessary reagents are included in the kit:
Use the AAVpro CRISPR/Cas9 Helper Free System to deliver expression cassettes for Cas9 and a target-specific sgRNA to hard-to-transfect cells. This system results in significantly higher levels of indel formation than what is typically obtained with plasmid-based delivery.
Our Lenti-X CRISPR/Cas9 System contains a Cas9 lentiviral plasmid and a separate pre-linearized sgRNA pladmis ready for insertion of your custom sgRNA sequences. We also provide our pre-lyophilized and ready to use Lenti-X Packaging Single Shots.
The Lenti-X Tet-On 3G CRISPR/Cas9 System contains a third-generation tetracycline-inducible system for tight control over Cas9 expression with very low background. This kit has all the components necessary for producing dox-inducible Cas9-expressing cell lines along with a pre-linearized sgRNA lentiviral plasmid ready for insertion of your custom sgRNA sequence.
The Guide-it CRISPR/Cas9 systems provide a simple method to generate a vector that will deliver a target-specific sgRNA, Cas9 nuclease, and a bright fluorescent marker to your target cells.
Use the Guide-it CRISPR/Cas9 Gesicle Production System to deliver Cas9 protein and a custom sgRNA to a broad range of cell types with very low cytotoxicity.
Use the Xfect RNA Transfection Reagent to efficiently transfect mammalian cells with messenger RNA (mRNA) and/or single guide RNA (sgRNA) with very low cytotoxicity.
Electroporation of mammalian cells with Cas9-sgRNA ribonucleoprotein complexes allows you to minimize off-target effects while facilitating a fast turnaround for highly efficient gene-editing experiments. Try our Guide-it Recombinant Cas9 (Electroporation-Ready) for your CRISPR/Cas9-mediated gene editing experiments.
Guide-it Recombinant Cas9 (Electroporation Ready) workflow.
The Guide-it Mutation Detection Kit provides a simple PCR-based technique to identify insertions and/or deletions generated in mammalian cells using CRISPR/Cas9 technology.
Guide-it Mutation Detection Kit workflow.
All necessary reagents are included in the kit:
The Guide-it Genotype Confirmation Kit provides a simple method to determine if a given clone has mutations in one allele (monoallelic), both alleles (biallelic), or is unchanged (wild type). Expedite the laborious process of screening a large number of clones for those with the desired genotype.
The Guide-it Indel Identification Kit provides a complete workflow for identifying the variety of indels created by CRISPR/Cas9 gene editing in a cell population.
One of the most powerful applications of genome editing is the introduction of nucleotide substitutions in specific genomic sites to mimic single-nucleotide polymorphisms (SNPs) related to human diseases or to generate stop codons that yield precise gene knockouts. However, screening hundreds of clones for a single edited nucleotide remains a challenge, especially in the absence of a corresponding phenotype. The Guide-it SNP Screening Kit allows the screening of hundreds of clones for single-nucleotide edits with a simple enzymatic assay.
The fast and simple workflow comprises PCR amplification of the genomic target site followed by an enzymatic assay using a structure-specific endonuclease that generates a fluorescent readout. This method performs comparably regardless of the nucleotide substitution being assayed, the zygosity of the clone, or the sequence of the targeted locus.
The Guide-it CRISPR Genome-Wide sgRNA Library System makes it easy to perform lentiviral-based, genome-wide phenotypic knockout screens using CRISPR/Cas9 in human cell lines. Included with the kit are expression systems for a genome-wide sgRNA library and Cas9 nuclease in our easy-to-use Lenti-X Single Shots format, enabling streamlined production of cell lines stably expressing both Cas9 and the sgRNA library.
In addition to the library, we also offer a customized NGS analysis kit for our sgRNA library system so you can perform NGS analysis of your screens with ease. The Guide-it CRISPR Genome-Wide sgRNA Library NGS Analysis Kit includes the necessary primers, reagents, and accessories to prepare an NGS-ready library from screened cell populations.
Doench, J.G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol. 34, 184–191 (2016).