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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > SMARTer RNA-Seq °³¿ä > [±â¼ú°³¿ä] SMARTer Technology

[±â¼ú°³¿ä] SMARTer Technology

-

RNA-Seq ±â¼ú°³¿ä

Single cell, ±Ø¼Ò·® RNA »ùÇà ºÐ¼®ÀÇ ±³°ú¼­

SMART¢ç technology


SMART¢ç ±â¼ú

SMART¢ç (Switching Mechanism at the 5¡¯ end of RNA Template) ±â¼úÀº
¿ªÀü»çÈ¿¼Ò (RTase)ÀÇ 1st strand cDNA ÇÕ¼ºÀÌ RNAÀÇ 5' ¸»´Ü¿¡ À̸£·¶
À» ¶§, Non-templated nucleotides (±×¸² 1ÀÇ XXXXX)¸¦ ºÎ°¡ÇÏ´Â È°¼º
(Terminal transferase activity)À» ÀÌ¿ëÇÑ´Ù. ºÎ°¡µÈ Non-templated
nucleotides¿¡ µ¶ÀÚ±â¼ú·Î °³¹ßÇÑ SMARTer¢ç switching oligo°¡ ƯÀÌÀûÀ¸
·Î °áÇÕÇϸç, À̸¦ ÁÖÇüÀ¸·Î cDNA ÇÕ¼ºÀ» Áö¼ÓÇÏ¿© ¾ç ¸»´Ü¿¡ SMARTer¢ç
Adapter°¡ ºÎ°¡µÈ 1st strand cDNA ÇÕ¼ºÀ» ¿Ï·áÇÑ´Ù (Template
switching activity).

SMART¢ç ±â¼úÀº º°µµÀÇ ligation °úÁ¤ ¾øÀÌ 1st strand cDNA ÇÕ¼º °úÁ¤ Áß
adapter¸¦ ºÎ°¡ÇÒ ¼ö ÀÖÀ¸¸ç, ÀÌ adapter¸¦ ÀÌ¿ëÇÏ¿© 1st strand cDNA¿¡
¼­¸¸ À¯·¡µÈ full-length double strand cDNA¸¦ ¾òÀ» ¼ö ÀÖ´Ù.



SMART¢ç ±â¼úÀÇ ÀåÁ¡

  • ¸Å¿ì ³ôÀº °¨µµ¿Í ÀçÇö¼º: ±Ø¼Ò·®, ¼Ò·® »ùÇà ´ëÀÀ
  • Full-length double strand cDNA ÇÕ¼º
  • °íÇ°ÁúÀÇ sequencing library Á¦ÀÛ °¡´É
  • °£´ÜÇÑ ÇÁ·ÎÅäÄÝ: ligation, enzyme cocktail ó¸® ºÒÇÊ¿ä



SMART¢ç ±â¼úÀÇ Àû¿ë

SMART¢ç ¹ýÀ» ÀÌ¿ëÇϸé 1st strand cDNA ÇÕ¼º °úÁ¤ Áß¿¡ adapter¿Í °°Àº
ƯÁ¤ ¼­¿­À» °£ÆíÇÏ°Ô ºÎ°¡ÇÒ ¼ö ÀÖ¾î, ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.


1
Next Generation Sequencing (NGS)
- È¿À²ÀÌ ³·Àº ligation ¹æ½Ä ´ë½Å SMART¢ç ±â¼úÀ» Àû¿ëÇϸé,
  °íÈ¿À²·Î NGS adapter, index¸¦ ºÎ°¡ÇÒ ¼ö ÀÖ´Ù.
- Àû¿ë: RNA-seq, DNA-seq
2
PCR, qPCR amplification
3
RACE (Rapid Amplification of cDNA Ends)
4
cDNA library construction