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Home > ÀüÁ¦Ç°º¸±â > ´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦ > His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦ > [¼±Åð¡À̵å] His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦

[¼±Åð¡À̵å] His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦

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[¼±Åð¡À̵å] His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦

´Ü¹éÁúÀ» Á¤Á¦Çϴµ¥ ÀÖ¾î °¡Àå °£´ÜÇÑ ¹æ¹ýÀº Ç×ü¸¦ ÀÌ¿ëÇÏ´Â °ÍÀÌÁö¸¸, ¸ðµç ´Ü¹éÁú¿¡ ´ëÇÑ Ç×ü°¡ È®¸³µÇÁö ¾Ê¾Ò±â ¶§¹®¿¡ ´Ü¹éÁú¿¡ Tag¸¦ ºÎÂøÇÏ´Â ¹æ¹ýÀÌ ¸¹ÀÌ »ç¿ëµÇ°í ÀÖ´Ù. ¸¹Àº ¿¬±¸ÀÚµéÀº ´Ü¹éÁúÀ» Á¤Á¦Çϱâ À§ÇØ His-tag¸¦ ÀÌ¿ëÇϴµ¥, his-tag´Â ÀÛÀº Å©±â·Î ´Ü¹éÁú ±â´É°ú ±¸Á¶¿¡ Å« ¿µÇâÀ» ¹ÌÄ¡Áö ¾ÊÀ¸¸ç, N- ȤÀº C- terminus ¾îµð¿¡ À§Ä¡Çϵç native protein, denatured proteinÀ» Á¤Á¦ÇÒ ¼ö Àֱ⠶§¹®ÀÌ´Ù.



ºÐ·ù
6xHis tag
6xHN tag
HAT tag
Å©±â
Small tag
6 amino acids
Histidine-rich peptide
12 amino acids
õ¿¬ À¯·¡ polyhistidine peptide
18 amino acids
À§Ä¡
C- / N-terminus C- / N-terminus N-terminus
Ư¡
³·Àº ´Ü¹éÁú ±â´É °£¼·
ÀúºÐÀÚ ´Ü¹éÁú¿¡ ÃÖÀû
³ôÀº ¿ëÇصµ
°íºÐÀÚ ´Ü¹éÁú¿¡ ÃÖÀû
³ôÀº ¿ëÇصµ
¾ÈÁ¤ÀûÀÎ ´Ü¹éÁú ¹ßÇö
°íºÐÀÚ ´Ü¹éÁú¿¡ ÃÖÀû
* º¸´Ù ÀÚ¼¼ÇÑ Á¤º¸´Â ¡®[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦¡¯¸¦ Âü°íÇϼ¼¿ä.



His-tag À¶ÇÕ ´Ü¹éÁúÀ» Á¤Á¦ÇÒ ¶§¿¡´Â ±Ý¼Ó ģȭ¼ºÀ» ÀÌ¿ëÇϸç, ÁÖ·Î nickel À̿°ú cobalt ÀÌ¿ÂÀ» ±â¹ÝÀ¸·Î ÇÑ resinÀÌ ¸¹ÀÌ »ç¿ëµÈ´Ù.
Ni2+Àº ´õ ¸¹Àº ¾çÀÇ ´Ü¹éÁúÀ» ¾òÀ» ¼ö ÀÖ°í, Co2+Àº ´õ ³ôÀº ¼øµµÀÇ ´Ü¹éÁúÀ» ¾òÀ» ¼ö ÀÖ´Ù.






Capturem¢â Ni Spin column
ºÐ·ù
TALON¢ç resin / His60 Ni resin
¿ø¸®
Small
Bed volume
Large
5~15 ºÐ
Á¤Á¦ ½Ã°£
60~120 ºÐ
°£ÆíÇÑ ÇÁ·ÎÅäÄÝ
³·Àº bed volumeÀ¸·Î °í³óµµ ´Ü¹éÁú ȹµæ
18Á¾ÀÇ ´Ù¾çÇÑ Ã·°¡Á¦ Àû¿ë °¡´É (¥âME, EDTA, DTT µî)
Ư¡
IMAC
(Immobilized Metal Affinity Chromatography)
Gravity/batch column, FPLC µî ´Ù¾çÇÑ ÀÀ¿ë
Capturem¢â His-tagged
Purification Miniprep Kit
¹ßÇö¿©ºÎ È®ÀÎ
Á¦Ç°
³ôÀº ¼øµµÀÇ
´Ü¹éÁú Á¤Á¦
TALON¢ç Metal Affinity Resin
Capturem¢â His-tagged
Purification Maxiprep
/ Large volumn type
Æí¸®ÇÏ°Ô scale-up
´õ¿í ¸¹Àº ¾çÀÇ
´Ü¹éÁú Á¤Á¦
His60 Ni Superflow Resin



 
Purity
Yield
Binding capacity
(§· protein / §¢ matrix)
¼Ò¿ä½Ã°£ (ºÐ)
Ease of use
Capturem His-Tagged
Purification Miniprep Kit
****
800 §¶/mini
spin column
100 §·/§¢
5 ºÐ
*****
His60 Ni resin
****
60 §·/§¢ resin
60 §·/§¢
60-120 ºÐ
***
TALON resin
*****
20 §·/§¢ resin
20 §·/§¢
60-120 ºÐ
***
Magnetic Beads
*****
15 §·/§¢ resin
15 §·/§¢
60 ºÐ
****



ºÐ·ù
Code
Á¦Ç°¸í
¿ë·®
Spin-Column
635710
Capturem¢â His-Tagged Purification Miniprep Kit
20ȸ
635713
Capturem¢â His-Tagged Purification Maxiprep Kit
6 ȸ
635715
Capturem¢â His-Tagged Purification Maxiprep Columns
25 ȸ
635724
Capturem¢â His-Tagged Purification Large Volume
4 ȸ
635714
Capturem¢â His-Tagged Purification 96
1 plate
635730
Capturem¢â His-Tagged Purification 24-Well Plate
1 plate
TALON resin
635501
TALON¢ç Metal Affinity Resin
10 §¢
635506
TALON¢ç Superflow Metal Affinity Resin
25 §¢
635682
HisTALON¢â Superflow Cartridges
5 §¢¡¿5
635509
TALON¢ç CellThru Resin
10 §¢
635601
TALON¢ç spin Columns
10 °³
635655
HisTALON¢â Gravity Columns
1 §¢¡¿5
635654
HisTALON¢â Gravity Columns Purification Kit
1 Kit
635651
HisTALON¢â Buffer Set
20 ȸ
635636
TALON¢ç Magnetic Beads
1 §¢¡¿2
635638
TALON¢ç Magnetic Beads Buffer Kit
1 Kit
Ni resin
635659
His60 Ni Superflow Resin
10 §¢
635675
His60 Ni Superflow Cartridges
1 §¢¡¿5
635674
His60 Ni Superflow Cartridge Purification Kit
1 §¢¡¿5
635657
His60 Ni Gravity Columns
1 §¢¡¿5
635658
His60 Ni Gravity Column Purification Kit
1 Kit
635665
His60 Ni Buffer Set
20 ȸ
635693
His60 Ni Magnetic Beads
1 §¢
His-tag °ËÃâÇ×ü
631210
6¡¿His mAb-HRP Conjugate
100 §¡
631212
6¡¿His Monoclonal Antibody (Albumin Free)
200 §¶
631213
6¡¿HN Polyclonal Antibody
200 §¡
±âŸ
631964
Magnetic Stand
1 Set