Proteins
Origins
Production(g/L)
References
Enzymes
¥á-amylase
B. licheniformis
3.7
¡¡
Sphingomyelinase
B. cereus
3
Xylanase
B. halodurans
0.2
CGTase
B. macerans
5
Chitosanase
B. circulans
1.4
Hyper thermo-stable protease
A. pernix
0.1
P. horikoshii
0.7
PDI
human
1
4)
Antigens
Surface antigen
E. rhusiopathiae
0.9
T. pallidum
0.8
Antibodies
VHH(Anti-NDOM)
lama
scFv(Anti-fluorescein)
mouse
Fab(Anti-erbB)
0.4
Cytokines
EGF
7
NGF
IFN-¥ã
chicken
0.5
6)
TNF-¥á
bovine
GM-CSF
GH
flounder
Component
Amount
pBIC DNA Set (Code HB310), 10 rxns
Linear Vector DNA
pBIC1 DNA
5 ¥ìg (100 ng/¥ìL)
pBIC2 DNA
pBIC3 DNA
pBIC4 DNA
Control (Insert DNA)
BLA
1 ¥ìg (80 ng/¥ìL)
Brevibacillus Competent Cells (Code HB116)
Brevibacillus Competent Cells
100 ¥ìL x 10
MT medium
1 mL x 10
Solution A
1 mL x 1
Solution B
1 mL x 2
Promoter
Portion of the 5¡Ç sequence upstream of the B. choshinensis P22 gene, which provides strong constitutive expression
Secretion Signal
pBIC1: Secretion signal for BbrPI from B. choshinensis
pBIC2: Secretion signal for a cell wall protein from B. brevis
pBIC3: Secretion signal for the P22 gene from B. choshinensis
pBIC4: Modified secretion signal for pBIC2
Terminator
46 bp termination signal inserted downstream from the cloning site
Rep
Encodes a protein related to plasmid replication (derived from pUB110)
Ori
Plasmid replication origin (derived from pUB110)
NmR
Neomycin resistance gene (selection marker)