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Home > Learning center > Cloning > ½ÇÇè °¡À̵å > Traditional CloningÀ» À§ÇÑ ´Ù¾çÇÑ Á¦Ç° ¶óÀÎ

Traditional CloningÀ» À§ÇÑ ´Ù¾çÇÑ Á¦Ç° ¶óÀÎ

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Traditional CloningÀ» À§ÇÑ ´Ù¾çÇÑ Á¦Ç° ¶óÀÎ

´ÙÄ«¶ó¿¡¼­´Â ´Ù¾çÇÑ molecular cloning workflow¿¡ ÇÊ¿äÇÑ °íÇ°ÁúÀÇ ´Ù¾çÇÑ ½Ã¾àÀ» Á¦°øÇÕ´Ï´Ù.
´ç»çÀÇ Á¦ÇÑÈ¿¼Ò, °í¼º´É DNA polymerase, DNA modifying enzyme, ligation kit´Â ¼º°øÀûÀΠŬ·Î´×À» À§ÇÑ ÈǸ¢ÇÑ µµ±¸°¡ µÉ °ÍÀÔ´Ï´Ù.

Á¦ÇÑÈ¿¼Ò overview
: »ç¿ë¹æ¹ý, star activity, reaction buffer È°¼º µî

PCR È¿¼Ò ¼±Åð¡À̵å
: ½ÇÇè¿¡ ¸Â´Â PCR È¿¼Ò ¼±ÅÃÇϱâ

¼ö½ÄÈ¿¼Ò
: DNA, RNA ligation µîÀ» À§ÇÑ ¼ö½ÄÈ¿¼Ò

Ligation cloning overview
: Traditional cloningÀ» À§ÇÑ ´Ù¾çÇÑ Á¦Ç°
: insert Å©±â, blunt/A-overhang µî¿¡ µû¸¥ ½Ã¾à ¼±ÅÃ

Ligation-free, DNA assemble!
In-Fusion ±â¼úÀ» »ç¿ëÇϸé 15ºÐ ¹ÝÀÀÀ¸·Î, 1°³ ¶Ç´Â ¿©·¯ °³ÀÇ PCR ´ÜÆíÀ» ´Ù¾çÇÑ linearized vector¿¡ 95% ÀÌ»óÀÇ ¼º°øÀ²·Î ½±°í ºü¸£°Ô ¹æÇ⼺À» °¡Áö°í Ŭ·Î´×ÇÒ ¼ö ÀÖ´Ù.



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