TaKaRa
로그인  |   회원가입  |   ID/PW 찾기  
제품명/제품코드
사이트내검색
Cell-Free DNA Library Preparation for Illumina® NGS Platforms

ThruPLEX® Plasma-seq Kit

-

제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
RB4490
ThruPLEX® Plasma-seq 12S Kit
관련학술 관심상품등록 구매하기
12회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400490 136655, 136704
Clontech
RB4491
ThruPLEX® Plasma-seq 48S Kit
관련학술 관심상품등록 구매하기
48 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400491 136655, 136704
Clontech
RB4492
ThruPLEX® Plasma-seq 96D Kit
관련학술 관심상품등록 구매하기
96 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400492 136655, 136704

  • Designed for cell-free DNA: newly formulated repair and ligation reagents with optimized protocols
  • High performance NGS libraries: high diversity with broad and reproducible GC coverage
  • Variable sample input: <1 ng to 30 ng of cell-free DNA
  • Fast and simple workflow: 3 steps in a single tube or well in 2 hours with no purification or sample transfer steps
  • Research applications: libraries from samples where results require high sensitivity including liquid biopsies, ctDNA, and targeted sequencing
  • Automation-friendly: Beckman® FXP Workstation
제품설명
ThruPLEX® Plasma-seq Kit은 혁신적인 ThruPLEX chemistry를 바탕으로 plasma로부터 추출한 cell-free DNA를 이용한 NGS용 library제작에 최적화된 제품으로, 본 제품을 이용하면 다양성이 높고, GC representation 유지하는 고퀄리티의 library를 제작할 수 있다. 본 제품으로 제작한 library는 CNV analysis, genome sequencing application 뿐 아니라 Agilent SureSelect®, Roche Nimblegen® SeqCap® EZ 등 주요 Target Enrichment platform과 같이 사용할 수 있다.
보관
Store at -20 °C.
Guarantee for 9 months at -20°C in a constant temperature freezer.
내용

Component name

Cap Color

12S Kit
12 Single Indexes

48S Kit
48 Single Indexes

96D Kit
96 Dual Indexes

Code

 

RB4490

RB4491

RB4492

용량

 

12

48

96

Preparation Buffer

Red

1 Tube

1 Tube

2 Tubes

Template Preparation Enzyme

Red

1 Tube

1 Tube

2 Tubes

Library Synthesis Buffer

Yellow

1 Tube

1 Tube

2 Tubes

Library Synthesis Enzyme

Yellow

1 Tube

1 Tube

2 Tubes

Library Amplification Buffer

Green

1 Tube

1 Tube

2 Tubes

Library Amplification Enzyme

Green

1 Tube

1 Tube

2 Tubes

Nuclease-Free Water

Clear

1 Tube

1 Tube

1 Tube

Indexing Reagents

Blue

12 Tubes

1 Single Index Plate (48S)

1 Dual Index Plate (96D)


※ ThruPLEX® Plasma-seq Kit single Index 서열
   * ThruPLEX Plasma-seq 12S Kit (RB4490)
   * ThruPLEX Plasma-seq 48S Kit (RB4491)
※ ThruPLEX® Plasma-seq Kit Index Guide 바로 가기


[그림1] ThruPlex® Plasma-Seq Kit Single-tube Workflow
Starting with 1 to 30 ng of cell-free DNA, ThruPLEX® Plasma-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.


[그림2] ThruPlex® Plasma-Seq Kit원리
ThruPLEX® Plasma-seq Kit technology is a 3-step reaction that is optimized for cell-free DNA. Cell-free DNA (1 ng to 30 ng) is first repaired in a highly efficient process. Background is reduced using double-stranded adapters with no single-stranded tails. Blunt-end ligation occurs with high-efficiency. Blocked 5’ ends reduce adapter-adapter ligation. Background is further reduced by destroying unused adapters after ligation. A high-fidelity amplification completes the reaction to generate indexed Illumina libraries.


[그림3] Highest Diversity, Fewest Unmapped Reads from Cell-free DNA
ThruPLEX® Plasma-seq Kit generated quality libraries with high diversity and a low number of duplicates and unmapped reads. Cell-free DNA was extracted from 3 plasma samples, and libraries were prepared at the amounts indicated as measured by Qubit®. The amount of mononucleosomal DNA in each sample as measured by the Bioanalyzer® corresponded to 0.09 ng, 0.62 ng, and 15.44 ng. Pooled libraries were sequenced on an Illumina NextSeq® 500 as a paired-end run with 17M to 25M reads per library, Duplication rates were calculated after down-sampling the data to 17 M reads per library.


[그림4] OutstandingTarget Enrichment Performance
ThruPLEX® Plasma-seq Kit libraries were captured at high efficiency and generated data with deep coverage of the kinome for mutation detection. Libraries were prepared from 3 plasma samples at input amounts of 5 ng, 6.5 ng, and 10 ng in triplicate, and targeted sequencing was carried out on an Illumina MiSeq® using samples enriched with the ClearSeq Human DNA Kinome Panel for SureSelectXT2. On average, 5M reads were generated per library. Selected bases were successfully captured bases that were in or within 250 bp of the baits.


[그림5] Reproducible, Unbiased GC Coverage
ThruPLEX® Plasma-seq Kit provided the most reproducible and unbiased GC coverage across the human genome. ThruPLEX® libraries showed minimal variability across 9 individual plasma samples tested. Libraries were prepared from cell-free DNA isolated from 1 ml of plasma samples and sequenced on an Illumina NextSeq® 500. Four separate plasma samples were used to construct the NEBNext® Ultra libraries.

Keyword : rubicon,R400490,R400491,R400492
-
Library preparation (for Illumina)
미량의 DNA로부터 Illumina NGS 분석용 Library 제..
> Low Input Library Prep Kit (for Illumina)
Illumina용 NGS Library 정량 키트
> Library Quantification Kit (for Illumina)
Single Cell Library Preparation for Illumina® N..
> PicoPLEX® DNA-Seq Kit
General Purpose Library Preparation for Illumina..
> ThruPLEX® DNA-seq Kit
Cell-Free DNA Library Preparation for Illumina®..
> ThruPLEX® Plasma-seq Kit
Illumina® NGS Library Preparation with Unique M..
> ThruPLEX® Tag-seq Kit
-