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제품명/제품코드
사이트내검색
General Purpose Library Preparation for Illumina® NGS Platforms

ThruPLEX® DNA-seq Kit

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
RB4523
ThruPLEX DNA-seq 6S (12 )Kit
관련학술 관심상품등록 구매하기
12회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400523 136704, 136654
Clontech
RB4428
ThruPLEX DNA-seq 12S (48)Kit
관련학술 관심상품등록 구매하기
48 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400428 136704, 136654
Clontech
RB4427
ThruPLEX DNA-seq 48S Kit
관련학술 관심상품등록 구매하기
48 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400427 136704, 136654
Clontech
RB4406
ThruPLEX DNA-seq 48D Kit
관련학술 관심상품등록 구매하기
48 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400406 136704, 136653
Clontech
RB4407
ThruPLEX DNA-seq 96D Kit
관련학술 관심상품등록 구매하기
96 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400407 136704, 136653

  
제품설명
ThruPLEX® DNA-seq Kit은 혁신적인 ThruPLEX chemistry를 이용하여 DNA library를 제작할 수 있는 제품이다. DNA library는 50 pg 수준의 극소량의 DNA로도 가능하며, multiplexing분석을 위하여 최대 96개까지의 index를 사용하여 민감도를 올릴 수 있다. 본 제품은 샘플 종류에 상관없이 fragmentation된 DNA라면 모두 적용이 가능하다(Genomic, biofluids such as cell-free DNA, DNA from FFPE materials, cDNA). ThruPLEX® DNA-seq Kit은 single tube(well) reaction으로 별도의 정제(purification)과정이나 tube교체가 필요 없으며, 약 2시간이면 완료할 수 있다.
제품에 따라 12, 48 , 96 single- 혹은 dual-read Illumina-compatible indexes가 포함되어 있으며, index에 따라 tube (6, 12) 혹은 pre-dispensed barcode microplate에 index가 제공된다.
ThruPLEX® DNA-seq Kit은 다양한 DNA-seq application과 RNA-seq을 위한 cDNA 그리고 ChIP-seq등에 사용될 수 있으며, 주요 target enrichment platform protocol도 함께 제공되고 있다.
보관
Store at -20 °C.
Guarantee for 9 months at -20°C in a constant temperature freezer.
내용
[ThruPLEX® DNA-seq Kit - Single index]

Component name

Cap Color

6S Kit
6 Single Indexes
12 Reactions

12S Kit
12 Single Indexes
48 Reactions

48S Kit
48 Single Indexes
48 Reactions

Code

 

RB4523

RB4428

RB4427

용량

 

12

48

48

Template Preparation Buffer

Red

1 Tube

1 Tube

1 Tube

Template Preparation Enzyme

Red

1 Tube

1 Tube

1 Tube

Library Synthesis Buffer

Yellow

1 Tube

1 Tube

1 Tube

Library Synthesis Enzyme

Yellow

1 Tube

1 Tube

1 Tube

Library Amplification Buffer

Green

1 Tube

1 Tube

1 Tube

Library Amplification Enzyme

Green

1 Tube

1 Tube

1 Tube

Nuclease-Free Water

Clear

1 Tube

1 Tube

1 Tube

Indexing Reagents

Blue

6 Tubes

12 Tubes

1 Single Index Plate (48S)


※ ThruPLEX® DNA-seq Kit single Index 서열
   * ThruPLEX DNA-seq 6S (12 )Kit (RB4523)
   * ThruPLEX DNA-seq 12S (48)Kit (RB4428)
   * ThruPLEX DNA-seq 48S Kit (RB4427)
※ ThruPLEX® DNA-seq Kit single Index Plate Guide(RB4427)

[ThruPLEX® DNA-seq Kit - Dual index]

Component name

Cap Color

48D Kit
48 Dual Indexes 48 Reactions

96D Kit
96 Dual Indexes 96 Reactions

Code

 

RB4406

RB4407

용량

 

48

96

Template Preparation Buffer

Red

1 Tube

2 Tubes

Template Preparation Enzyme

Red

1 Tube

2 Tubes

Library Synthesis Buffer

Yellow

1 Tube

2 Tubes

Library Synthesis Enzyme

Yellow

1 Tube

2 Tubes

Library Amplification Buffer

Green

1 Tube

2 Tubes

Library Amplification Enzyme

Green

1 Tube

2 Tubes

Nuclease-Free Water

Clear

1 Tube

1 Tube

Indexing Reagents

 

1 Dual Index Plate
(48B or 48D)

1 Dual Index Plate
(96A or 96D)


※ ThruPLEX® DNA-seq Kit Dual Index Plate Guide(RB4406, RB4407)


[그림1] ThruPLEX® DNA-seq Kit Single- tube Workflow
Starting with as little as 50 pg of fragmented double-stranded DNA or cDNA, ThruPLEX DNA-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. The streamlined workflow prevents sample loss and is performed in about 2 hours in a single tube or well preventing sample loss and enhancing positive sample identification.


[그림2] ThruPLEX® DNA-seq Kit 기술
ThruPLEX® technology is a 3-step reaction that starts with fragmented double-stranded DNA (0.05 ng to 50 ng) which is repaired in a highly efficient process. Background is reduced using double-stranded adaptors with no single-stranded tails. Blunt end ligation occurs with high-efficiency. Blocked 5’ends reduce adaptor-adaptor ligation. Background is further reduced by destroying unused adaptors after ligation.


[그림4] ThruPLEX® DNA-seq Kit와 타사 제품과의 비교
ThruPLEX® DNA-seq Kit yielded more diverse libraries (left) with less input DNA and generated fewer duplicates (right) at 25M read pairs than other kits. NGS libraries were prepared with the indicated amount of reference gDNA using the library preparation kits indicated and sequenced on a MiSeq®, v3 flow cell.


[그림5] ThruPLEX® DNA-seq Kit Minimizes GC Bias
Relative coverage of the human genome as a function of %GC (50 ng input DNA). (Left) ThruPLEX® DNA-seq Kit has excellent coverage throughout the entire range. (Right) ThruPLEX® DNA-seq Kit (gold) performs as well as or better than other kits tested. Note that the optimal coverage should be equal to 1 in these graphs. GC composition distribution of the human genome is represented by the red bars.

Keyword : R400523,R400428,R400427,R400406,R400407,rubicon
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