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Illumina® NGS Library Preparation with Unique Molecular Tags

ThruPLEX® Tag-seq Kit

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
RB4584
ThruPLEX® Tag-seq 6S(12)Kit
관련학술 관심상품등록 구매하기
12회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400584 136656, 136704
Clontech
RB4585
ThruPLEX®Tag-seq 48S Kit
관련학술 관심상품등록 구매하기
48 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400585 136656, 136704
Clontech
RB4586
ThruPLEX® Tag-seq 96D Kit
관련학술 관심상품등록 구매하기
96 회
가격문의   제조사 페이지로 바로가기 구 Rubicon No : R400586 136656, 136704

  • Results you can trust. ThruPLEX® Tag-seq technology provides up to 16 million molecular tags to correct sequencing errors providing confident variant detection.
  • Discover more, cost effectively. Use with hybridization-based target enrichment systems to examine hundreds of genes, including mutations and structural variants, simultaneously.
  • High quality libraries the first time, every time. Unparalleled ease of use reduces user error and contamination with our single-tube, 2-hour, 3-step workflow.
  • Time-saving bioinformatics solutions. Reach results quickly with cloud-based software from Curio Genomics or open-source scripts available for data processing.
  • Precious samples go further. Use less DNA to analyze samples once too low to detect. ThruPLEX® Tag-seq works with input amounts from 1 ng to 50 ng of cfDNA or fragmented dsDNA to accommodate a wide range of samples.
제품설명
ThruPLEX® Tag-seq Kit은 molecular tag와 ThruPLEX chemistry를 결합시켜, 샘플 index가 부가된 Illumina NGS library를 제작할 수 있는 제품이다.
ThruPLEX® Tag-seq Kit의 Stem-Loop adaptor는 1600만 개의 unique molecular tag(UMT)을 포함하고 있어서, PCR 증폭 전에 각각의 DNA 단편에 UMT을 부가 할 수 있다. 그 결과, NGS 데이터 분석에서 위양성 비율을 감소시켜 보다 정확한 결과를 얻을 수 있다.
보관
Store at -20 °C.
Guarantee for 9 months at -20°C in a constant temperature freezer.
내용

Component name

Cap Color

6S Kit
6 Single Indexes

48S Kit
48 Single Indexes

96D Kit
96 Dual Indexes

Code

 

RB4584

RB4585

RB4586

용량

 

12

48

96

Template Preparation Buffer

Red

1 Tube

1 Tube

2 Tubes

Template Preparation Enzyme

Red

1 Tube

1 Tube

2 Tubes

Library Synthesis Buffer

Yellow

1 Tube

1 Tube

2 Tubes

Library Synthesis Enzyme

Yellow

1 Tube

1 Tube

2 Tubes

Library Amplification Buffer

Green

1 Tube

1 Tube

2 Tubes

Library Amplification Enzyme

Green

1 Tube

1 Tube

2 Tubes

Nuclease-Free Water

Clear

1 Tube

1 Tube

1 Tube

Indexing Reagents

Blue

6 Tubes

1 Single Index Plate (48S)

1 Dual Index Plate (96D)


※ ThruPLEX® Tag-seq Kit single Index 서열
   * ThruPLEX Tag-seq 6S Kit (RB4584)
   * ThruPLEX Tag-seq 48S Kit (RB4585)
※ ThruPLEX® Tag-seq Kit Index Guide 바로 가기


[그림1] Single-Tube, Three step workflow helps your precious samples go further
Starting with 1 to 50 ng of DNA, ThruPLEX® Tag-seq Kit creates indexed libraries in 3 simple steps: end repair, adapter ligation, and high-fidelity library amplification. No purification or sample transfer steps are required. The streamlined workflow is performed in 2 hours in a single tube or well, preventing sample loss and enhancing positive sample identification.


[그림 2] ThruPLEX® Technology generates high quality libraries the first time, every time
ThruPLEX® technology is a 3-step reaction that starts with fragmented double-stranded DNA or cell-free DNA which is repaired in a highly efficient process. Background is reduced using double-stranded adaptors with no single-stranded tails. Blunt end ligation occurs with high-efficiency. Blocked 5' ends reduce adapter-adapter ligation.


[그림 3] Reduce background for results you can trust
ThruPLEX® Tag-seq libraries were prepared from 30 ng of the 1% Horizon cfDNA standard. Following Agilent SureSelect target enrichment and sequencing to achieve ~10,000X coverage, data was analyzed with the Curio Genomics bioinformatics platform. Using the raw reads, the EGFR mutation (yellow dot) is obscured by background errors (left). The mutation is readily identified when UMTs are utilized in data processing to correct for errors (right).


[그림 4] Dramatically increase the signal to noise ratio to discover more
Data from either the 30 ng Horizon cfDNA library(A) or a ThruPLEX® Tag-seq library prepared from 10 ng Horizon cfDNA standard, enriched with a custom 240 KB panel (NimbleGen SeqCapEZ) and sequenced on a HiSeq 2500 to achieve ~5,000X coverage. Analysis was performed using the Curio Genomics bioinformatics platform.

Keyword : rubicon,R400584,R400585,R400586
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