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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > iPS cell Á¦ÀÛ ¹× °ËÃâ > Human ES / iPS ¼¼Æ÷°ü·Ã Ç×ü

Human ES / iPS ¼¼Æ÷°ü·Ã Ç×ü

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
µ¥ÀÌÅÍ°¡ ¾ø½À´Ï´Ù

  • hESC ȤÀº reprogrammingµÈ hiPSC Ŭ·ÐÀÇ Ãʱ⠰ËÃâ
  • ¸é¿ª ¿°»ö, flow cytometry, magnetic cell separation ÀÌ¿ë °¡´É
  • ¼¼Æ÷ÀÇ °íÁ¤(fixation), ¸·Åõ°ú(permeabilization) ó¸® ºÒÇÊ¿ä
  • ¼¼Æ÷¿¡ ´ëÇÑ ³·Àº ¼¼Æ÷ µ¶¼º
  • Human somatic cell°ú fibroblast¿¡ ´ëÇÑ ³·Àº ±³Â÷¼º : SSEA Ç×ü¿Í TRA Ç×ü¿¡ ºñÇØ ³·Àº ±³Â÷¼º
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ´Ù´É¼º Áٱ⼼Æ÷ ¿¬±¸¿¡ ƯȭµÈ ¹ÌºÐÈ­ human ES ¼¼Æ÷¸¦ Ç׿øÀ¸·Î ÇÑ ¸¶¿ì½º ´ÜŬ·Ð Ç×ü·Î, µ¿°á °ÇÁ¶ÇüÅ·ΠÁ¦°øµÈ´Ù. Human ES ¼¼Æ÷¿Í human iPS ¼¼Æ÷ÀÇ Ç¥¸é Ç׿øÀ» ÀνÄÇÏ´Â Ç×üÀ̸ç, ÇÇ´õ(feeder) ¼¼Æ÷ ȤÀº ºÐÈ­µÈ ¼¼Æ÷·ÎºÎÅÍ ÀÚ±âºÐ¸®(magnetic separation) ¶Ç´Â cell sorter¸¦ ÀÌ¿ëÇÏ¿©, human ES ¼¼Æ÷¿Í human iPS ¼¼Æ÷ ºÐ¸®°¡ °¡´ÉÇÏ´Ù. º» Á¦Ç°¿¡ ÀÇÇÑ human iPS ¼¼Æ÷¿¡ ´ëÇÑ µ¶¼ºÀº °üÂûµÇÁö ¾Ê¾Ò´Ù.
¼ö¼Û
4 ¡É (³ìÀÎ ÈÄ 4 ¡É ÀúÀåÇÒ °æ¿ì 6ÁÖ°£ ¾ÈÁ¤)

Figure 1: Flow cytometric quantification of undifferentiated hES cells using hES-Cellect (red) and ES-Cellect (green) compared to SSEA-1 (blue) and TRA 1-60 (purple).

Figure 2: Undifferentiated human pluripotent stem cells in Cellartis feeder free DEF-CS (culture system) characterized by ES-Cellect¢â immunocytochemical staining in green (A), nuclei stained in blue (B).

Figure 3: Immunocytochemistry staining of mouse ES cells on mouse feeder cells using ES-Cellect¢â


Keyword : Cellartis,Áٱ⼼Æ÷Ç×ü,fibroblast,stemcell,antibody

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