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Home > 전제품보기 > Stem Cell > 줄기세포 배양배지 > Xeno-Free 3D Spheroid DEF-CS Culture Medium
Xeno Free의 human iPS/ES세포의 3D배양용 배지

Xeno-Free 3D Spheroid DEF-CS Culture Medium

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Cellartis
Y30047
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium, w/o antibiotics
관련학술 관심상품등록 구매하기
1 Kit
가격문의   Y30047_Cellartis DEF-CS Xeno-Free 3D Spheroid Culture Medium User Manual_030917.pdf

  • 배양 용기 면적에 의존하지 않는 3D spheroid culture에서 human iPS/ES세포 배양 scale up에 유용
  • 최적화된 프로토콜과 배지 첨가제에 의한 뛰어난 확대 배양 효율 (800배 /11일)
  • 3D분화유도 전 배양 프로토콜에도 최적
  • 안정된 핵형과 분화유도능 유지
  • Xeno-free: 동물이나 인간 유래 성분 불포함
제품설명
본 제품은 인간 유래 다능성 줄기 세포(human ES / iPS 세포)의 3D spheroid 배양용 배지로 동물이나 인간 유래 성분을 포함하지 않는 chemically defined media이다. 배양용기 면적에 의존하지 않고, 배양 스케일 업에 유용하다. 본 제품에서 배양한 인간 유래 다능성 줄기 세포는 분화 유도 능력 및 핵형을 유지하고 있다.

※ Basal Medium에는 GMP grade 제품(Code Y30071)도 있다.
본 제품 이용 시 Cellartis® DEF-CS™ 500 Xeno-Free 3D Spheroid Additives (Code Y30048)와 조합해서 사용하십시오.
내용

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o antibiotics (Code Y30047)

Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics

500 ml

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid additives

1 Set; 배지 첨가제
(Code Y30048)

- DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000×)

500 ㎕

- DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000×)

200 ㎕

- DEF-CS Xeno-Free 3D Spheroid Additive 3 (500×)

400 ㎕

보존
Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o antibiotics : 4℃
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid additives:-15℃ 이하

Application data

그림 1. High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates grown under 4% O2 perfusion conditions were sequentially passaged three times by mechanical disruption (protocol adapted from Otsuji et al. 2014); cells were counted at passage on days 4 and 7, and at the final time point on day 11, to determine the expansion factor relative to the initial seeding number.


그림 2. High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates were differentiated into definitive endoderm cells using a four-day protocol, then further differentiated into either lung progenitors (left), hepatospheres (middle), or beta cells (right) via respective 11-15 day differentiation protocols. qPCR data (not shown) indicated increases in FOXA2 and NKX2.1 expression in lung progenitors; CYP3A4, AFP, and CYP1A in hepatospheres; and PDX1 and NKX6.1 in beta cells.


그림 3. High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). Representative images of aggregates at days 1, 2, and 5 after seeding with 2.5 x 105 cells/ml. Viable cells were labeled with fluorescein diacetate (green) and dead cells with propidium iodide (red).


그림 4. High-quality hiPS cell aggregates were grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). After expansion of hiPS cells as aggregates, cells were harvested on day 4 and expression of OCT4, SSEA-4, TRA-1-60 (pluripotency markers) and SSEA-1 and SOX17 (differentiation markers) was analyzed by immunostaining.

Keyword : spheroid,iPS,ES,줄기세포배양,3D,3Dculture,줄기세포배지,xeno-free
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