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event
 Á¦¸ñ : [À̺¥Æ® Á¾·á] [ÇÁ·Î¸ð¼Ç] Cloning, NGS, typing¿¡ ÃÖÀû, long range PCR ÃÖ°­ÀÚ (~4/19±îÁö ÇÒÀÎ Áß!)
ÀÛ¼ºÀÏ : 2024.04.01
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ

Long range PCRÀÇ ÃÖ°­ÀÚ, PrimeSTAR¢ç GXL DNA Polymerase

 

Cloning, NGS, typing µî¿¡ ÃÖÀû!

´Ù¾çÇÑ ±æÀÌ¿Í GC contents¸¦ °íÈ¿À²·Î ÁõÆøÇÏ´Â long range PCRÀÇ ÃÖ°­ÀÚ,

PrimeSTAR¢ç GXL DNA Polymerase¸¦ 20% ÇÒÀΰ¡·Î ±¸¸ÅÇϼ¼¿ä.

-      ÇÁ·Î¸ð¼Ç ±â°£:  2024³â 4¿ù 19ÀÏ (±Ý) ±îÁö

 

  • ¹ü¿ë¼º ³ôÀº High Fidelity PCR È¿¼ÒÀÇ Ç¥ÁØ
  • µ¶ÀÚÀûÀÎ ½ÅÀåÀÎÀÚ(elongation factor)¸¦ ÷°¡ÇÏ¿© long fragment ÁõÆø ½ÇÇö (human gDNA ~30 kb)
  • GC rich targetµµ È¿À²ÀûÀ¸·Î ÁõÆø
  • Anti-Taq antibody·Î hot start PCR Àû¿ë

 

Code

Á¦Ç°¸í

¿ë·®

Ư¡

R050A/B

PrimeSTAR¢ç GXL DNA Polymerase

250 U/1,000 U

È¿¼Ò, buffer, dNTP ±¸¼º, hot-start

R051A/B

PrimeSTAR¢ç GXL Premix

200 ȸ/800 ȸ

2x premix, hot-start

R052A/B

PrimeSTAR¢ç GXL Premix Fast, Dye plus

200 ȸ/800 ȸ

2x premix (dye plus),

Fast PCR (½ÅÀå½Ã°£~10ÃÊ), hot-start

*±Ø°­ÀÇ Á¤È®µµ¸¦ °¡Áø °í¼Ó PCR È¿¼Ò 

PrimeSTAR¢ç Max DNA Polymerase Ver.2 (Code R047A/B) µµ 20% ÇÒÀÎ Áß!

Á¦Ç° Á¤º¸ È®ÀÎ ¹× »ùÇà ½ÅûÇϱâ (¸µÅ©)

 

 

*ÇÔ²² »ç¿ëÇϸé ÁÁÀº TA-cloning kit: 20% ÇÒÀÎ Áß (~4/19±îÁö)

Code

Á¦Ç°¸í

¿ë·®

Ư¡

High fidelity PCR È¿¼Ò·Î ÁõÆøÇÑ insertµµ °£´ÜÇÏ°Ô TA cloning °¡´É

6019

Mighty TA-cloning Reagent Set for PrimeSTAR¢ç

20ȸ

A-tailing ½Ã¾à, ligation premix,

T-vector·Î ±¸¼º

ÃÊ°£´Ü ligationÀ» À§ÇÑ 1 solution premix

6023

DNA Ligation Kit £¼Mighty Mix£¾

1 Kit

Ligation¿¡ ÇÊ¿äÇÑ ½Ã¾àÀÌ ¸ðµÎ Æ÷ÇÔµÈ premix



Á¦Ç° ¹× µ¥¸ð ¹®ÀÇ´Â ´ÙÄ«¶óÄÚ¸®¾Æ Áö¿ª ´ë¸®Á¡ (´ë¸®Á¡ ¾È³») ¶Ç´Â °í°´Áö¿ø (02-2081-2510)À¸·Î ¿¬¶ôÁÖ¼¼¿ä!

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