º¸´Ù °íÇ°ÁúÀÇ RNA¸¦ °í¼öÀ²·Î ÇÕ¼ºÇÒ ¼ö ÀÖ½À´Ï´Ù.
PrimeCap¢â T7 RNA Polymerase (low dsRNA)
mRNA´Â ÇöÀç ´Ù¾çÇÑ °¨¿°º´¿¡ ´ëÇÑ ¹é½Å ¿¬±¸ »Ó¸¸ ¾Æ´Ï¶ó ¾Ï Ä¡·á µîÀÇ ´Ù¾çÇÑ ºÐ¾ß¿¡¼ ³Î¸® °ËÅäµÇ°í ÀÖ½À´Ï´Ù.À̶§ ´ëºÎºÐ T7 RNA Polymerase¸¦ ÀÌ¿ëÇÑ IVT (; in vitro transcription)¸¦ ÅëÇØ mRNA¸¦ ÇÕ¼ºÇÏÁö¸¸ ±× °úÁ¤¿¡¼ ¸ñÀûÇÏ´Â ssRNA ¿Ü¿¡µµ dsRNA°¡ ºÎ»ê¹°·Î È¥ÀԵ˴ϴÙ.ÀÌ·± dsRNA´Â ¼¼Æ÷ ³»ÀÇ Æ¯Á¤ ¼ö¿ëü¿¡ ÀÇÇØ °¨ÁöµÇ¸é¼ ¸é¿ª¿ø¼ºÀ» À¯¹ßÇÕ´Ï´Ù.µû¶ó¼ ¾ÈÀüÇÏ°í È¿°úÀûÀÎ mRNA ¹é½Å ¹× Ä¡·áÁ¦¸¦ »ý»êÇÏ·Á¸é dsRNAÀÇ »ý¼ºÀ» ÃÖ¼ÒÈÇØ¾ß ÇÕ´Ï´Ù.
PrimeCap¢â T7 RNA Polymerase (low dsRNA) (Code 2560A)Àº ±âÁ¸ T7 RNA Polymerase ver.2.0 (Code 2541A)ÀÇ ³ôÀº ¼öÀ²À» À¯ÁöÇϸç dsRNAÀÇ »ý¼ºÀ²À» ÃÖ¼ÒÈÇÑ º¯ÀÌüÀÔ´Ï´Ù (±×¸² 1).º¸´Ù °íÇ°ÁúÀÇ RNA¸¦ °í¼öÀ²·Î ÇÕ¼ºÇÒ ¼ö ÀÖ¾î mRNA ¹é½Å ¹× Ä¡·áÁ¦ ºÐ¾ßÀÇ ¿¬±¸ °³¹ß¿¡ ÀûÇÕÇÕ´Ï´Ù.
±×¸² 1. FLuc mRNA (¾à 1.9 kb)ÀÇ ÇÕ¼º ¼öÀ² (¿ÞÂÊ) ¹× dsRNA »ý¼ºÀ² (¿À¸¥ÂÊ).
°ü·ÃÁ¦Ç°
Code |
Á¦Ç°¸í |
¿ëµµ |
6146 |
Template Vector (BspQ
I) for T7 mRNA Synthesis |
scarless mRNA ÇÕ¼º¿ë template Á¦ÀÛ |
1227A |
IVT¿¡ ÃÖÀû Type IIS Á¦ÇÑÈ¿¼Ò | |
2460A |
Capping enzyme (uncapped
-> Cap0) | |
2470A |
mRNA Cap 2-O-Methyltransferase |
Capping enzyme (Cap0 ->
Cap1) |
3417A |
RNA ÇÕ¼º ÈÄ Àü±â¿µµ¿ ½Ã Marker |
ÀÌ ¹Û¿¡ ´Ù¾çÇÑ Takara Á¦Ç°µéÀ» 4¿ù 19ÀϱîÁö 20% ÇÒÀΰ¡·Î ±¸ÀÔÇÒ ¼ö ÀÖ´Â ±âȸ¸¦ ³õÄ¡Áö ¸¶¼¼¿ä!
Á¦Ç° °ü·ÃµÈ ¹®ÀÇ»çÇ×Àº °í°´Áö¿ø (02-2081-2510, support@takara.co.kr) ·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.
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