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´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ

0.5-10 kb ssRNA Ladder Marker

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
3417A
0.5-10 kb ssRNA Ladder Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 §¡ (25 lanes)
141,600¿ø 
177,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3417A_DS.v2311Da.pdf

Á¦Ç° ¼³¸í
0.5-10 kb ssRNA Ladder Marker´Â 8°³ÀÇ single-stranded RNA (ssRNA) ´ÜÆíÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Â Àü±â¿µµ¿ ¿ë ladderÀÌ´Ù. ´Ù¾çÇÑ Å©±âÀÇ RNA È®Àο¡ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç ¸ñÀû¿¡ µû¶ó ºñº¯¼º ¶Ç´Â º¯¼º agarose°Ö ¸ðµÎ Àû¿ëÀÌ °¡´ÉÇÏ´Ù.

´ÜÆí Å©±â (kb): 10, 8, 6, 4, 3, 2, 1, 0.5 kb
- 3 kbÀÇ Band°¡ ÁøÇØ Àü±â¿µµ¿½Ã ½ÃÀμºÀÌ ÁÁ°í ¹êµå »çÀÌÁî ±¸ºÐÀÌ ¿ëÀÌÇÏ´Ù.

º¸Á¸: -80 ¡É
¿ë·®: 50 §¡
³»¿ë

±¸¼ºÇ°

¿ë·®

Çü»ó

0.5-10 kb ssRNA Ladder Marker

50 §¡

1 mM Sodium Citrate, pH 6.4

RNA Loading Buffer*

1 §¢

Formamide 96%, EDTA 20mM

6 x Dye**

1 §¢

Orange G 0.5%, EDTA 1mM

* °³ºÀ ÈÄ -20 ¡É º¸°ü °¡´É
**°³ºÀ ÈÄ -20 ¡É ȤÀº 4 ¡É º¸°ü °¡´É
¿ëµµ
- RNA Àü±â¿µµ¿ ½Ã molecular size marker·Î »ç¿ë
»ç¿ë»óÀÇ ÁÖÀÇ
- 0.5-10 kb ssRNA Ladder Marker´Â »ç¿ë ÈÄ ½Å¼ÓÇÏ°Ô -80 ¡É¿¡ º¸°ü ¹Ù¶ø´Ï´Ù.
- RNA Loading Buffer³ª 6x Dye¸¦ 0.5-10 kb ssRNA Ladder Marker¿¡ ÷°¡ÇÑ »óÅ·δ º¸°üÀÌ ºÒ°¡´ÉÇÕ´Ï´Ù.
- RNase ¿À¿°À» ¹æÁöÇϱâ À§ÇØ RNA ½ÇÇè Àü¿ëÀÇ ¸¶ÀÌÅ©·ÎÆÄÀÌÆê ÆÁ ¹× Æ©ºê¸¦ »ç¿ëÇÏ°í, ÀÏȸ¿ë Àå°©À» »ç¿ëÇϽñ⠹ٶø´Ï´Ù. Ãß°¡·Î Plasmid¿Í °°ÀÌ RNase¸¦ ´Ù·ç´Â ½ÇÇè°ú´Â °ø°£À» ºÐ¸®Çϱ⠹ٶø´Ï´Ù.
- »ùÇà ssRNAÀÇ ¼­¿­À̳ª ¾ç¿¡ µû¶ó Àü±â¿µµ¿ ½Ã size´Â Â÷ÀÌ°¡ ÀÖÀ» ¼öµµ ÀÖ½À´Ï´Ù.
°ü·ÃÁ¦Ç°
- Çѹø¿¡ 200 §¶ mRNA ÇÕ¼º, °í¼öÀ² in vitro transcription: Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)
- IVT¿ë Plasmid templateÀ» °£´ÜÇÏ°Ô Á¦ÀÛÇÏ´Â Kit: Cloning Kit for mRNA Template (Code 6143)
- Capping enzyme, uncapped RNA -> cap-0 RNA: Vaccinia Capping Enzyme (Code 2460A)
- Capping enzyme, cap-0 RNA -> cap-1 RNA: mRNA Cap 2-O-Methyltransferase (Code 2470A)
- IVTÀü vectorÀÇ ¼±ÇüÈ­¿¡ »ç¿ëÇÏ´Â Type IIS Á¦ÇÑÈ¿¼Ò: BspQ I (Code 1227A)
- BspQ I Àνļ­¿­ÀÌ ÀÖ´Â IVT template vector: Template Vector (BspQ I) for T7 mRNA Synthesis (Code 6146)
- mRNA ¹ßÇöÀ» Boost½Ã¾àÀÌ Æ÷ÇÔµÈ transfection reagent: TransIT¢ç-mRNA Transfection Kit (Code MIR 2250)

Keyword :

in vitro transcription
>
°íÇ°Áú °í¼öÀ² in vitro transcriptionÀ» À§ÇÑ ..
Takara IVTpro¢â mRNA Synthesis System
>
dsRNA »ý¼ºÀÌ Àû¾î ÀÓ»óºÐ¾ßÀÇ ¿¬±¸°³¹ß¿¡ ÀûÇÕ..
PrimeCap¢â T7 RNA Polymerase (low dsRNA)
>
Poly(A) ÀÌÈÄ Ãß°¡¼­¿­ÀÌ ¾ø´Â scarless mRNA ..
Template Vector (BspQ I) for T7 mRNA Synthesis
>
RNAÀÇ 5¡¯-¸»´Ü¿¡ Cap (Cap 0)À» ºÎ°¡ÇÏ´Â È¿¼Ò
Vaccinia Capping Enzyme
>
RNAÀÇ 5¡¯ ¸»´ÜÀ» Cap 0 ¡æ Cap 1·Î º¯È¯ÇÏ´Â ..
mRNA Cap 2-O-Methyltransferase
>
´Ù¾çÇÑ ¿Âµµ¿¡¼­ ¹ÝÀÀÇÒ ¼ö ÀÖ´Â Capping enzym..
Faustovirus Capping Enzyme (S17)
>
Poly(A) ¹× ¹Ýº¹ ¼­¿­À» Æ÷ÇÔÇÏ´Â À¯ÀüÀÚÀÇ Å¬..
Takara Stable Competent Cells
>
RNA ÇÕ¼º ¼öÀ²ÀÌ Çâ»óµÈ
T7 RNA Polymerase ver.2.0
>
Àú³óµµ DTT¿¡¼­µµ ¾ÈÁ¤ÀûÀÎ RNase inhibitor
Recombinant RNase Inhibitor ver.2.0
>
DNA, RNA ÇÕ¼º ¹ÝÀÀÀ» ÃËÁø
Pyrophosphatase (inorganic)
>
IVT Àû¿ë¿¡ ÃÖÀû, Type IIS Á¦ÇÑÈ¿¼Ò
BspQ I
>
´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ
0.5-10 kb ssRNA Ladder Marker