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DNA, RNA ÇÕ¼º ¹ÝÀÀÀ» ÃËÁø

Pyrophosphatase (inorganic)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
2450A
Pyrophosphatase (inorganic)
°ü·ÃÇмú ±¸¸ÅÇϱâ
10U
77,000¿ø  2450A_DS.pdf

¡ØÀÌ Á¦Ç°Àº ÀÏ¹Ý ¿¬±¸¿ë ½Ã¾à (RUO grade) ¿Ü¿¡µµ Ä¡·áÁ¦ ¹× ¹é½ÅÀÇ Á¦Á¶ °øÁ¤ ÇÁ·Î¼¼½º °³¹ßÀ» ¸ñÀûÀ¸·Î ÇÑ High Quality (HQ) grade ½Ã¾àÀÌ ÆǸŵǰí ÀÖ½À´Ï´Ù.
HQ grade Á¦Ç° °ü·Ã ¹®ÀÇ»çÇ×Àº ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (TEL. 02-2081-2510, Email. support@takara.co.kr)À¸·Î ¿¬¶ô ÁÖ¼¼¿ä.
- È¿¼ÒÀÇ Ç°Áúµî±Þ RUO/HQ/GMP ¹Ù·Î°¡±â
Á¦Ç°¼³¸í
º» È¿¼Ò´Â inorganic pyrophosphate (PPi)ÀÇ ºÐÇظ¦ Ã˸ÅÇÏ¿© orthophosphate¸¦ »ý¼ºÇÑ´Ù. RNA¿Í DNAÀÇ ÇÕ¼º °úÁ¤¿¡¼­ »ý±â´Â inorganic pyrophosphate (PPi)¸¦ ºÐÇØÇؼ­ DNA¿Í RNAÀÇ ÇÕ¼º ¹ÝÀÀÀ» ÃËÁøÇÒ ¼ö ÀÖ´Ù.
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- in vitro transcription¿¡¼­ÀÇ RNA ¼öÀ² Çâ»ó
- PCR ¹ÝÀÀ ÃËÁø
º¸Á¸ -20¡É
³óµµ 0.1 U/§¡
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Escherichia coli carrying a plasmid containing the gene for Saccharomyces cerevisiae (yeast) inorganic pyrophosphatase
ÀϹÝÀûÀÎ ¼ºÁú
- Áú·®: ¾à 66 kDa (homodimer)
- º¸Á¶ÀÎÀÚ: Mg2+
È°¼ºÀÇ Á¤ÀÇ
25 ¡É¿¡¼­ 1ºÐ°£ PPi¿¡¼­ 1 ¥ìmolÀÇ ÀλêÀ» »ý¼ºÇÏ´Â È¿¼ÒÀÇ ¾çÀ» 1UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀÁ¦ Á¶¼º

100 mM

 

Tris-HCl (pH7.2)

2 mM

 

MgCl2

2 mM

 

inorganic pytophosphate (PPi)

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- º» È¿¼Ò´Â °úµµÇÑ vortexingÀº ÇÇÇϽñ⠹ٶø´Ï´Ù
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- RNA ¹é½Å ¿¬±¸¸¦ À§ÇÑ Takara IVTpro¢â mRNA Synthesis System (Code 6141)
- RNA ÇÕ¼º ¼öÀ²ÀÌ ¾÷±×·¹À̵åµÈ T7 RNA Polymerase ver.2.0 (Code 2541A)
- mRNA Àü¿ë transfection ½Ã¾à TransIT¢ç-mRNA Transfection Kit (Code MIR 2250)

Keyword :

in vitro transcription
>
°íÇ°Áú °í¼öÀ² in vitro transcriptionÀ» À§ÇÑ ..
Takara IVTpro¢â mRNA Synthesis System
>
dsRNA »ý¼ºÀÌ Àû¾î ÀÓ»óºÐ¾ßÀÇ ¿¬±¸°³¹ß¿¡ ÀûÇÕ..
PrimeCap¢â T7 RNA Polymerase (low dsRNA)
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Poly(A) ÀÌÈÄ Ãß°¡¼­¿­ÀÌ ¾ø´Â scarless mRNA ..
Template Vector (BspQ I) for T7 mRNA Synthesis
>
RNAÀÇ 5¡¯-¸»´Ü¿¡ Cap (Cap 0)À» ºÎ°¡ÇÏ´Â È¿¼Ò
Vaccinia Capping Enzyme
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RNAÀÇ 5¡¯ ¸»´ÜÀ» Cap 0 ¡æ Cap 1·Î º¯È¯ÇÏ´Â ..
mRNA Cap 2-O-Methyltransferase
>
´Ù¾çÇÑ ¿Âµµ¿¡¼­ ¹ÝÀÀÇÒ ¼ö ÀÖ´Â Capping enzym..
Faustovirus Capping Enzyme (S17)
>
Poly(A) ¹× ¹Ýº¹ ¼­¿­À» Æ÷ÇÔÇÏ´Â À¯ÀüÀÚÀÇ Å¬..
Takara Stable Competent Cells
>
RNA ÇÕ¼º ¼öÀ²ÀÌ Çâ»óµÈ
T7 RNA Polymerase ver.2.0
>
Àú³óµµ DTT¿¡¼­µµ ¾ÈÁ¤ÀûÀÎ RNase inhibitor
Recombinant RNase Inhibitor ver.2.0
>
DNA, RNA ÇÕ¼º ¹ÝÀÀÀ» ÃËÁø
Pyrophosphatase (inorganic)
>
IVT Àû¿ë¿¡ ÃÖÀû, Type IIS Á¦ÇÑÈ¿¼Ò
BspQ I
>
´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ
0.5-10 kb ssRNA Ladder Marker
>
BspQ IÀ» »ç¿ëÇÑ °í¼öÀ² IVT ½Ã½ºÅÛ
(BspQ I) IVTpro¢â ½Ã½ºÅÛ
>
Low dsRNA Polymerase¸¦ »ç¿ëÇÑ °í¼öÀ² IVT ½Ã..
(PrimeCap¢â T7) IVTpro¢â ½Ã½ºÅÛ