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event
 Á¦¸ñ : [À̺¥Æ® Á¾·á] [ÇÁ·Î¸ð¼Ç] ÃʽÉÀÚ Ä£È­ÀûÀÎ mRNA ÇÕ¼º ½Ã½ºÅÛ, Takara IVTpro¢â 20% ÇÒÀÎ Áß!!
ÀÛ¼ºÀÏ : 2024.02.23
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ
ÃʽÉÀÚ Ä£È­ÀûÀΠmRNA ÇÕ¼º ½Ã½ºÅÛ, Takara IVTpro¢â 20% ÇÒÀÎ Áß!!

 

´ç»ç¿¡¼­´Â mRNA ÇÕ¼º ½ÇÇè (IVT; in vitro transcription)À» À§ÇØ ÇÕ¼º ¹× Á¤Á¦»Ó¸¸ ¾Æ´Ï¶ó template plasmid Á¦ÀÛ±îÁö ÇϳªÀÇ ½Ã½ºÅÛÀ¸·Î ÆǸÅÇÏ°í ÀÖ½À´Ï´Ù.

Template plasmid µðÀÚÀÎÀ» °£´ÜÇÏ°Ô »ý·«ÇÒ ¼ö ÀÖ°í ´Ù¾çÇÑ Á¶°Ç¿¡¼­ ¼öÀ²ÀÌ ³ô±â¿¡ ½ÇÇè ÃʽÉÀÚµµ °£´ÜÇÏ°Ô ½ÇÇèÀ» ¼¼ÆÃÇÒ ¼ö ÀÖ½À´Ï´Ù.

 

Takara IVTpro¢â mRNA Synthesis System (Code 6141)

- IVT¿ë template plasmid Á¦ÀÛ (Cloning Kit for mRNA Template (Code 6143))°ú ÃÖ°í ¼öÀ² IVT ¹× Á¤Á¦ (Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144))ÀÇ ¼¼Æ®Á¦Ç° ÀÔ´Ï´Ù.


±×¸² 1. IVT ½ÇÇè ¼ø¼­ ¹× Ãßõ Á¦Ç° 


1. Template plasmid Á¦ÀÛ

- Cloning Kit for mRNA Template (Code 6143)Àº º°µµÀÇ plasmid µðÀÚÀÎ ¾øÀÌ ¹ßÇö½ÃÅ°°íÀÚ ÇÏ´Â À¯ÀüÀÚÀÇ coding sequence (CDS)¸¸ cloningÇØ mRNAÀÇ templateÀ» Á¦ÀÛÇÒ ¼ö ÀÖ½À´Ï´Ù.

À̸¦ »ç¿ëÇØ ÇÕ¼ºµÈ mRNA´Â humanÀ̳ª mouse µîÀÇ Æ÷À¯·ùÀÇ ¼¼Æ÷¿¡ Àû¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.

 

2. ÃÖ°í ¼öÀ² IVT ¹× Á¤Á¦

- Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)Àº dsDNA Template¿¡¼­ °í¼öÀ²·Î mRNA¸¦ ÇÕ¼ºÇϴ IVT KitÀÔ´Ï´Ù.

Ÿ»ç ´ëºñ ³ôÀº ¼öÀ²À» °¡Áö°í ÀÖ¾î mRNA ´ç »ý»ê ´Ü°¡¸¦ ³·Ãâ ¼ö ÀÖ´Â Á¡ÀÌ ÀåÁ¡ÀÔ´Ï´Ù.


±×¸² 2. ½ÃÁßÀÇ ´Ù¸¥ IVT Kit¿ÍÀÇ mRNA ÇÕ¼º ¼öÀ² ºñ±³ 

 

°ü·ÃÁ¦Ç°

Code

Á¦Ç°¸í

¿ëµµ

6146

Template Vector (BspQ I) for T7 mRNA Synthesis

scarless mRNA ÇÕ¼º¿ë template Á¦ÀÛ

2460A

Vaccinia Capping Enzyme

Capping enzyme (uncapped -> Cap0)

2470A

mRNA Cap 2-O-Methyltransferase

Capping enzyme (Cap0 -> Cap1)

3417A

0.5-10 kb ssRNA Ladder Marker

RNA ÇÕ¼º ÈÄ Àü±â¿µµ¿ ½Ã Marker

   
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