´ç»ç¿¡¼´Â mRNA ÇÕ¼º ½ÇÇè (IVT; in vitro transcription)À» À§ÇØ ÇÕ¼º ¹× Á¤Á¦»Ó¸¸ ¾Æ´Ï¶ó template plasmid Á¦ÀÛ±îÁö ÇϳªÀÇ ½Ã½ºÅÛÀ¸·Î ÆǸÅÇÏ°í ÀÖ½À´Ï´Ù.
Template
plasmid µðÀÚÀÎÀ» °£´ÜÇÏ°Ô »ý·«ÇÒ ¼ö ÀÖ°í ´Ù¾çÇÑ Á¶°Ç¿¡¼ ¼öÀ²ÀÌ ³ô±â¿¡ ½ÇÇè ÃʽÉÀÚµµ
°£´ÜÇÏ°Ô ½ÇÇèÀ» ¼¼ÆÃÇÒ ¼ö ÀÖ½À´Ï´Ù.
Takara IVTpro¢â
mRNA Synthesis System (Code 6141)
- IVT¿ë template plasmid Á¦ÀÛ (Cloning Kit for mRNA Template (Code
6143))°ú ÃÖ°í ¼öÀ² IVT ¹× Á¤Á¦ (Takara IVTpro¢â
T7 mRNA Synthesis Kit (Code 6144))ÀÇ ¼¼Æ®Á¦Ç° ÀÔ´Ï´Ù.
±×¸² 1. IVT ½ÇÇè ¼ø¼ ¹× Ãßõ Á¦Ç°
1. Template plasmid Á¦ÀÛ
- Cloning Kit for mRNA Template (Code 6143)Àº º°µµÀÇ plasmid µðÀÚÀÎ ¾øÀÌ ¹ßÇö½ÃÅ°°íÀÚ ÇÏ´Â À¯ÀüÀÚÀÇ coding sequence (CDS)¸¸ cloningÇØ mRNAÀÇ templateÀ» Á¦ÀÛÇÒ ¼ö ÀÖ½À´Ï´Ù.
À̸¦ »ç¿ëÇØ ÇÕ¼ºµÈ mRNA´Â humanÀ̳ª mouse µîÀÇ Æ÷À¯·ùÀÇ ¼¼Æ÷¿¡ Àû¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
2. ÃÖ°í ¼öÀ² IVT ¹× Á¤Á¦
- Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)Àº dsDNA Template¿¡¼ °í¼öÀ²·Î mRNA¸¦ ÇÕ¼ºÇÏ´Â IVT KitÀÔ´Ï´Ù.
Ÿ»ç ´ëºñ ³ôÀº ¼öÀ²À» °¡Áö°í ÀÖ¾î mRNA ´ç »ý»ê ´Ü°¡¸¦ ³·Ãâ ¼ö ÀÖ´Â Á¡ÀÌ ÀåÁ¡ÀÔ´Ï´Ù.
±×¸² 2. ½ÃÁßÀÇ ´Ù¸¥ IVT Kit¿ÍÀÇ mRNA ÇÕ¼º ¼öÀ² ºñ±³
°ü·ÃÁ¦Ç°
Code |
Á¦Ç°¸í |
¿ëµµ |
6146 |
Template Vector (BspQ I) for T7 mRNA Synthesis |
scarless
mRNA ÇÕ¼º¿ë template Á¦ÀÛ |
2460A |
Capping enzyme
(uncapped -> Cap0) | |
2470A |
mRNA Cap 2-O-Methyltransferase |
Capping enzyme
(Cap0 -> Cap1) |
3417A |
RNA ÇÕ¼º ÈÄ Àü±â¿µµ¿ ½Ã Marker |