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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Retrovirus > iPS Cell Generation¢â All-in-One Vector
ÇÊ¿äÇÑ À¯ÀüÀÚ¸¦ ÇϳªÀÇ vector¿¡ žÁ¦½ÃÄÑ Human iPS Cell Á¦ÀÛ È¿À²ÀÌ Çâ»ó

iPS Cell Generation¢â All-in-One Vector

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Á¦Ç°¼³¸í
º» Á¦Ç°Àº human iPS¼¼Æ÷ À¯µµ¿ë ÀçÁ¶ÇÕ retrovirus vector Á¦ÀÛÀ» À§ÇÑ plasmid DNA ÀÌ´Ù. °íÈ¿À², °í¹ßÇö À¯ÀüÀÚµµÀÔ¿ë retrovirus vectorÀÎ pDON-5 DNA (Code 3658)¿¡ iPS¼¼Æ÷ À¯µµ¿ë À¯ÀüÀÚÀÎ OCT3/4¿Í SOX2*, KLF4, LIN28°ú NANOG*ÀÌ Thosea asigna virusÀÇ2A¹è¿­*1 ¸¦ »ðÀÔ ½ÃÄÑ 1°³ÀÇ º¤ÅÍ¿¡ žÀçµÇ°í ÀÖ´Ù. º» º¤ÅÍ¿Í Retrovirus Packaging Kit Ampho (Code 6161)¿¡ Æ÷ÇԵǴ pGP Vector ¹× pE-ampho Vector¸¦ retrovirus Á¶Á¦¿ë 293 cell ¶Ç´Â 293T cell µî¿¡ co-transfection ÇÏ¿© iPS cell À¯µµ¿ë retrovirus¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ÀÌ·¸°Ô Á¦ÀÛµÈ ÀçÁ¶ÇÕ retrovirus´Â RetroNectin (Recombinant Human Fibronectin Fragment) (Code T100A/B)¿¡ ´ëÇÑ Ä£È­¼ºÀÌ ³ô±â ¶§¹®¿¡, ¸ñÀû¼¼Æ÷¿¡ À¯ÀüÀÚ µµÀԽà RetroNectinÀ» °íÁ¤È­ÇÑ laboratory dish¸¦ ÀÌ¿ëÇÏ´Â °ÍÀ¸·Î, ³ôÀº À¯ÀüÀÚµµÀÔ È¿À²À» ¾òÀ» ¼ö ÀÖ°í, iPS¼¼Æ÷À¯µµ È¿À²ÀÇ Çâ»óÀ» ±â´ëÇÒ ¼ö ÀÖ´Ù.

*1 5°³ÀÇ À¯ÀüÀÚ´Â ´ÜÀÏ mRNA·Î Àü»çµÇÁö¸¸, ¹ø¿ªµÉ ¶§¿¡ 2A ¹è¿­ ³»ÀÇ Æ¯Á¤ÀÇ À§Ä¡¿¡¼­ ºÐ¸®µÇ¾î °á°úÀûÀ¸·Î 5°³ÀÇ ´Ü¹éÁúÀÌ °ÅÀÇ ºñ½ÁÇÑ ¼öÁØÀ¸·Î ¹ßÇöÇÑ´Ù°í »ý°¢ÇÒ ¼ö ÀÖ´Ù.

Vector´Â ´ÙÀ½°ú À¯ÀüÀÚ Á¤º¸¸¦ ¹ÙÅÁÀ¸·Î ±¸ÃàÇß´Ù.
À¯ÀüÀÚ À̸§ GenBank Accession No .
OCT4 NM_002701.4
SOX2 NM_003106.2
KLF4 NM_004235.3*2
LIN28 NM_024674.4
NANOG NM_024865.2

*2 ÇöÀç´Â NM_004235.4·Î °»½ÅµÇ¾î ÀÖ¾î CDSÀÇ 5¡¯Ãø¿¡ 9 ¾Æ¹Ì³ë»ê¿¡ »ó´çÇÏ´Â ¹è¿­ÀÌ Ãß°¡µÇ¾ú´Ù. º» Á¦Ç°ÀÇ KFL4 À¯ÀüÀÚ´Â N¸»´ÜÀÇ 9¾Æ¹Ì³ë»êÀ» Æ÷ÇÔÇÏÁö ¾ÊÁö¸¸, iPS ¼¼Æ÷ À¯µµ¿¡ À¯È¿ÇÑ °ÍÀ» È®ÀÎÇÏ¿´´Ù.
Ư¡
  • OCT3/4, SOX2, KLF4, LIN28, NANOG À¯ÀüÀÚ¸¦ ÇϳªÀÇ vector¿¡ žÀç
  • iPS¼¼Æ÷ À¯µµ¿ë ÀçÁ¶ÇÕ retrovirus¸¦ È¿À²ÀûÀ¸·Î Á¶Á¦
  • RetroNectinÀÇ º´¿ëÀ¸·ÎiPS ¼¼Æ÷ À¯µµ È¿À²µµ ¾÷
³»¿ë
pDON-5 OKSLN DNA 0.5 ¥ìg/¥ìl 20 §¡
±æÀÌ 9,954 bp
º¸Á¸ -20¡É
pDON-5 OKSN DNAÀÇ ±¸Á¶


Keyword :

Retrovirus
>
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
>
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
>
ÇÊ¿äÇÑ À¯ÀüÀÚ¸¦ ÇϳªÀÇ vector¿¡ žÁ¦½ÃÄÑ Hum..
iPS Cell Generation¢â All-in-One Vector
>
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
>
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
>
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
>
Virus »óû¾×¿¡¼­ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
>
Retrovirus vector¸¦ ÀÌ¿ëÇÏ¿© Mammalian cell..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤