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Tet-Off Cell Lines

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Clontech
 631152
 HEK 293 Tet-Off Advanced Cell Line
관련학술 구매하기 LMO제품  라이선스  		각 1 ml
(Package) 		1,725,000원  제조사 페이지로 바로가기 x33147, x33149
Clontech
 631156
 HeLa Tet-Off Advanced Cell Line
관련학술 구매하기 LMO제품  라이선스  		각 1 ml
(Package) 		1,725,000원  제조사 페이지로 바로가기 x33147, x33149

[주의] 동결된 세포주의 해동(Thawing) 시, 세포 생존율 향상을 위하여 collagen-coated plate 또는 flaks를 사용하십시오.
       추후 세포 배양 시에도 세포주의 부착율(adherence)이 좋지 않다면 collagen-coated plate 또는 flask를 사용하는 것을 권장합니다.

NOTE: We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.

 Tet-Off and Tet-Off Advanced inducible cell lines are host cell lines that greatly simplify the task of setting up tetracycline-inducible gene expression systems which are activated by removing doxycycline (Dox) from the culture medium (Figure 1; 1, 2). Our Tet-On® Advanced and Tet-On inducible cell lines are also available, which are activated by adding Dox to the culture medium.
These rigorously tested clonal cell lines:
  • Stably express either the second-generation Tet-Off Advanced transactivator protein or the original Tet-Off transactivator protein
  • Allow you to induce high-level expression of your gene-of-interest by removing (Dox) from the culture of transfected cells (Figure 2)
  • Can save you up to two months of time in developing a complete Tet-controlled system.

Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTight in pTRE-Tight), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.

Our PC12 Tet-Off Cell Line responds normally to nerve growth factor (NGF). For more details, see our report Neural Differentiation in Tet-Inducible PC12 Cell Lines.
References
1. Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89:5547-5551.
2. Gossen, M., et al. (1995) Science 268:1766-1769.
Components
  • Tet-Off or Tet-Off Advanced Cell Line
  • CHO AA8-Luc Tet-Off Control Cell Line
  • Tet System Approved FBS (50 ml)
Storage Conditions
196°C
Cell Line 취급 주의사항 (클릭)

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