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Home > 전제품보기 > Inducible System · Yeast hybrid > Tetracycline 유도 발현 시스템 > Tet-On 3G Tatracycline 발현 유도 시스템

Tet-On 3G Tatracycline 발현 유도 시스템

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Clontech
631164
Tet-On 3G Inducible Expression System (with ZsGreen1)
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Tet-On 3G Inducible Expression System (with mCherry)
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Tet-On 3G Inducible Expression System (EF1α Version)
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Clontech
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Tet-On 3G Inducible Expression System
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Lenti-X Tet-On 3G Inducible Expression System
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Retro-X Tet-On 3G Inducible Expression System
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Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)
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Clontech
631337
Tet-On 3G Bidirectional Inducible Expression System
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Clontech
631338
Tet-On 3G Bidirectional Inducible Expression System (with mCherry)
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2,005,000원  제조사 페이지로 바로가기 17569
Clontech
631339
Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)
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2,005,000원  제조사 페이지로 바로가기 17569
Clontech
631340
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version)
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1,818,000원  제조사 페이지로 바로가기 17569
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631341
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)
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Clontech
631342
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)
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Clontech
631346
Tet-On 3G Inducible Expression System (EF1alpha, Bicistronic)
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Clontech
631347
Tet-On 3G Inducible Expression System (EF1alpha, mCherry)
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2,005,000원  제조사 페이지로 바로가기 17569
Clontech
631363
Lenti-X Tet-On 3G Inducible Expression System (EF1alpha Version)
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3,237,000원  제조사 페이지로 바로가기 49091

Tet-On Inducible Expression - The 3rd Generation
The Tet-On 3G Tetracycline Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The 3G system offers a significant improvement over Tet-On and Tet-On Advanced by further reducing basal expression and increasing sensitivity to doxycycline, a tetracycline analogue (Table I, Figures 1-3).
표 1
Tet-On 3G: Lowest Background Ever, Highest Sensitivity, Highest Fold Induction
Lowest Background
Mutations within the PTRE3G promoter have reduced background expression from this inducible promoter by 5-20 fold compared to our previous tightest promoter PTight (Figure 1).

Highest Sensitivity
Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox), so that at very low Dox concentrations (between 5-10 ng/ml), expression can be 100-150 fold higher when using a Tet-On 3G system, compared to Tet-On Advanced (Figure 3). An overnight exposure to as little as 10 ng/ml of Dox generates very high expression levels (Figure 4).

Highest Fold Induction
Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher (Figure 2).

We have observed unprecedented fold induction levels even in transient cotransfection experiments-up to a 27,000 fold difference between the induced and uninduced expression levels in MCF7 cells (not shown).

To learn about the mechanism of tetracycline-controlled inducible expression and the changes made to both the transactivator and TRE promoter creating the Tet-On 3G Inducible Expression System, visit our Tet-On 3G resource site or read the inducible expression technical note.


Figure 1. The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On Advanced Inducible Expression Systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G System produces far lower background expression in the absence of Dox (inset).

Keyword :
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Tetracycline 유도 발현 시스템
> 양방향성 Tet 유도 발현 Vectors
> 항생물질
> Lenti-X Tet-On 3G Inducible Expression System
> Linear 및 Plasmid 형 선택 Marker
> pTet-tTS Vectors
> pTRE-Tight Vectors
> Tet 시스템 발현 벡터
> Tet System Approved FBS
> Tet-Advanced Cell Lines
> Tet-Express – Fast, Simple Tet-Inducible Expression
> Tet-Inducible Dual Expression Systems
> Tet-Off® & Tet-Off Advanced Cell Lines
> Tet-On 3G Tatracycline 발현 유도 시스템
> Tet-On Advanced & Tet-Off Advanced Tetracycline 유도발현 시스템
> TetR 항체
All-in-one vector 형태의 Tet-inducible system
> Tetracycline Inducible Expression - Tet-One Systems
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