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Yeast Plasmid Isolation Kit

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Clontech
630467
Easy Yeast Plasmid Isolation Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
(Package)
679,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32634

  • Æí¸®ÇÑ spin-column ¹æ½Ä
  • Zymolyase È¿¼Ò¸¦ »ç¿ëÇÏ¿© È¿°úÀûÀ¸·Î ¼¼Æ÷º®À» ºÐÇØ
  • ªÀº ½Ã°£¿¡ ´õ ¸¹Àº DNA Á¤Á¦
Easy Yeast Plasmid Isolation Kit´Â yeast (S. cerevisiae)¿¡¼­ plasmid DNA¸¦ °£´ÜÇÏ°í È¿À²ÀûÀ¸·Î ÃßÃâÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. Á¶ÀÛ °úÁ¤Àº Zymolyase¸¦ »ç¿ëÇÏ¿© yeast ¼¼Æ÷º®À» ºÐÇØÇÏ¿© spheroplast (¼¼Æ÷º®À» ¸ðµÎ Á¦°ÅÇÑ ±Õü)¸¦ ¸¸µé°í, SDS/alkaline ¹æ¹ýÀ¸·Î ¼¼Æ÷¸¦ ÆļâÇÏ¿© Spin columnÀ¸·Î plasmid DNA¸¦ Á¤Á¦ÇÑ´Ù. Á¤Á¦ÇÑ plasmid DNA´Â ´ëÀå±Õ¿¡ ÇüÁú ÀüȯÇÏ¿© Áõ½Ä ÈÄ ´ë·®À¸·Î plasmid¸¦ Á¤Á¦Çϰųª Á÷Á¢ PCRÀ» À§ÇÑ ÁÖÇüÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.


Figure 1. A simple wash, lyse, and spin column procedure for isolating plasmid DNA from yeast.
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Box 1

Zymolyase Suspension (25 mg/ml)

 

1 §¢

ZYM Buffer

 

12 §¢


Box 2

10 mM EDTA

 

28 §¢

Y1 Resuspension Buffer

 

15 §¢

Y2 Lysis Buffer

 

15 §¢

Y3 Neutralization Buffer

 

20 §¢

Y4 Wash Buffer Concentrate

 

6 §¢

YE Elution Buffer

 

15 §¢

Yeast Plasmid Spin Columns

 

50 ea

Collection Tubes (2 ml)

 

50 ea

RNase A (Lyophilized)

 

6 §·

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Box 1: -20¡É¿¡¼­ º¸°ü, ù Çص¿ ÈÄ 4¡É º¸°ü

Box 2: room temperature

°¢ Á¦Ç°ÀÇ ÀÚ¼¼ÇÑ ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.

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