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Clontech
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631323
|
Xfect Protein Transfection Reagent
|
30 rxns
|
733,000¿ø
|
|
|
Clontech
|
631324
|
Xfect Protein Transfection Reagent
|
100 rxns
|
1,435,000¿ø
|
|
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Xfect Protein Transfection Reagent´Â ClontechÀÌ µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ´Ü¹éÁú¿ë transfection ½Ã¾àÀÌ´Ù. ¸ñÀû ´Ü¹éÁúÀÌ ¼¼Æ÷¸·À» Åë°ú ÇÒ ¼ö ÀÖµµ·Ï ¼¼Æ÷ Åõ°ú¼º ÆéƼµå(CPP£»cell-penetrating peptide)¸¦ ä¿ëÇØ ¸ñÀû ´Ü¹éÁúÀ» °íÈ¿À²·Î ¼¼Æ÷·Î µµÀÔÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¼¼Æ÷ µ¶¼ºÀÌ ³·°í ´Ü¹éÁúÀÇ È°¼ºÀ» À¯ÁöÇÑ »óÅ·Π¼¼Æ÷¿¡ µµÀÔÇÒ ¼ö ÀÖ´Ù(±×¸² 1). º» Á¦Ç°Àº ±âÁ¸ÀÇ ´Ü¹éÁú µµÀÔ È¿À²ÀÌ ³·´Ù°í ¾Ë·ÁÁø human À¯·¡ÀÇ ºÎÀ¯ ¼¼Æ÷³ª mouse À¯·¡ ES¼¼Æ÷ µî¿¡µµ È¿°úÀûÀÌ´Ù. Plasmid DNA¸¦ ÀÌ¿ëÇÑ À¯ÀüÀÚ µµÀԽà plasmid DNA·ÎºÎÅÍ mRNA Àü»ç¸¦ °ÅÃÄ ¸ñÀû ´Ü¹éÁúÀÌ ¼¼Æ÷³»¿¡¼ ¹ßÇö µÉ ¶§±îÁö 12~24½Ã°£À» ÇÊ¿ä·Î ÇÏÁö¸¸, º» Á¦Ç°À» ÀÌ¿ëÇϸé Ȱ¼ºÀÌ À¯ÁöµÈ ´Ü¹éÁúÀ» 1~2½Ã°£¸¸¿¡ ¼¼Æ÷¿¡ Á÷Á¢ µµÀÔÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù.
±×¸² 1. Simple, rapid protein transfection with Xfect Protein Transfection Reagent. Xfect¡¯s cell-penetrating activity enables proteins to be transported across membranes of mammalian cells.
±×¸² 2. Xfect Protein Transfection Reagent delivers up to 8X more active b-galactosidase into HeLa cells than the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent or Product C was used to transfect Jurkat cells with various amount of b-galactosidase (b-gal) according to the manufacturers'recommended protocol. One hour post-transfection, the cells were assayed for b-gal activity using Clontech¡¯s Luminescent b-galactosidase Detection Kit II (Cat. No. 631712). Xfect Protein Transfection Reagent displayed a far higher signal for b-gal than did Product C. Panel B. HeLa cells transfected with 4 ¥ìg of b-gal demonstrated higher efficiency and a higher amount of b-gal protein per cell when transfection was performed with Xfect Protein Transfection Reagent (middle panel) than with Product C (righthand panel).
±×¸² 3. Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent yields higher transfection efficiencies than Product C across a broad range of mammalian cells, including a number of rodent and human cell lines, hard-to-transfect human suspension cells, and mouse embryonic stem cells. The cells were transfected with 4 §¶ of recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express) using Xfect Protein Transfection Reagent or Product C according to the manufacturers' recommended protocol. Transfection efficiency was assessed by flow cytometry one hour post-transfection. Panel B. HeLa transfected with 5 §¶ of rAcGFP1 using Product C or Xfect Protein Transfection Reagent.
±×¸² 4. Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells. HeLa cells were seeded in 96-well plate (3 x 104 cells per well) one day prior to transfection. The cells were transfected with 0.25 §¶ of beta-galactosidase using Product P or Xfect Protein Transfection Reagent for 4 hours. A WST-1 proliferation assay was performed to assess cytotoxicity in the transfected samples relative to untreated HeLa cells.
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¡¤ Xfect Protein Transfection Reagent
¡¤ Xfect Protein Buffer
¡¤ Deionized H2O
¡¤ Beta-Galactosidase Control
Keyword :

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