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형광 단백질 · Reporter
형광 단백질의 용도에 따른 분류
> 세포 주기 Monitoring Vector（Fucci）
세포 주기 Monitoring Vector（Fucci）
Cell Cycle Monitoring Vectors (Fucci)
Single cells or complex tissues-no fixation required!
Easily and precisely track cell-cycle progression in live cells
Monitor phase transitions in cultured cells or live tissue
Visualize cell shape during phase transitions
Label whole cells or just nuclei
Monitor stably expressed phase reporters over long time periods
* 최신 메뉴얼과 관련 자료는 상단 가격표 우측의 [more] 아이콘을 누르시면 확인 가능 합니다.
Clontech’s cell cycle reporter vectors let you monitor cell cycle progression in living cells, in real-time, without fixation.
Our cell cycle reporter vectors deliver fluorescent, ubiquitination-based, cell-cycle indicators (Fucci; 1, 2) that allow you to identify cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1. Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan fluorescent tag. These probes allow precise, visual evaluation of the cell cycle phase (Figure 1).
Monitor Cell Cycle and Shape in Real-Time
Our Fucci cell cycle reporters let you label just the nucleus, or both the nucleus and cytoplasm-allowing visualization of cell shape. Although most Fucci probes label only cell nuclei, we offer a truncated version of hGeminin that is able to migrate to the cytoplasm between S and M phases, enabling the morphology of the cell to be visualized (Table I; 3).
A Variety of Fucci Probes and Delivery Options
We offer retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes (see Table I). Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin. Our retroviral vectors let you express Fucci probes in a wide variety of mammalian cells. Once integrated into the host genome, these vectors provide stable, heritable expression of the probes.
The plasmid-based vector, pTRE-CellCycle (Figure 3), allows tightly controlled, tetracycline (Tet)-inducible expression of two Fucci probes from a bidirectional promoter that can be induced using any Tet-On or Tet-Off technology. Expression of these probes causes cell nuclei to turn red during G1 phase and cyan during S, G2, and M phases, allowing complete visual tracking of the cell cycle. For more information on the latest Tet System technologies, visit the Tet-On 3G Product Page.
Use Clontech’s Fucci vectors to monitor cell cycle progression in live cells, as well as cell cycle phase distributions in a cell population. Our Fucci vectors even let you monitor cell shape as the cells transition through the cell cycle.
Figure 2. Retroviral delivery is available for a variety of Fucci probes.
Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.
1.Sakaue-Sawano, A. et al. (2008) Cell 132(3):487-98.
2.Newman, R.H. & Zhang, J. (2008) Chem Biol. 15(2):97-98.
3.Sakaue-Sawano, A. et al. (2008) Chem Biol. 15(12):1243-1248.
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Takara에도 다 있다!
PAGE, Western Blot용 시약
안전한 단백질 염색시약
TaKaRa CBB Protein Safe Stain
Methanol, acetic acid없이 안전한 염색
간편하게 D.W destaining 가능
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원하는 감도에 따라 선택하는
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고순도 spCas9으로 고효율 유전자 편집
Glycerol 농도가 낮아 세포독성 최소화
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Western BLoT Stripping Buffer
Cloning용 premix 특별할인중!
정확도 높은 PCR premix &
편리한 2x ligation premix