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TransIT®-BrCa Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 5504
TransIT-BrCa Transfection Reagent
관련학술 관심상품등록 구매하기
0.4 ml
가격문의   ml058_transit_brca_transfection_reagent.pdf
Mirus
MIR 5500
TransIT-BrCa Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml
가격문의  
Mirus
MIR 5505
TransIT-BrCa Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml×5
가격문의  
Mirus
MIR 5506
TransIT-BrCa Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml×10
가격문의  

  • Broad Spectrum DNA Delivery - Achieve high expression levels in breast cancer cell types including: MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468, and T47D.
  • Formulated for Low Cellular Toxicity - Maintain cell density and reduce experimental biases due to toxicity.
  • Superior Transfection Efficiency - TransIT-BrCa outperforms Lipofectamine 2000, in multiple breast cancer cell lines.
Specifications

Storage

Store TransIT-BrCa Reagent tightly capped at -20°C. Before each use, warm to room temperature and vortex gently.

Product Guarantee

6 months from the date of purchase, when properly stored and handled.

Data


Figure 1. TransIT-BrCa Transfection Reagent Exhibits Higher Luciferase Expression in Breast Cancer Cells Compared to Other Transfection Reagents. Breast Cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D, were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 μg of plasmid DNA per well. Luciferase expression was measured at 24 hours post-transfection using a standard assay. Error bars represent the standard deviation of triplicate wells.



Figure 2. High Efficiency Delivery of Plasmid DNA in Breast Cancer Cell Types. TransIT®-BrCa Transfection Reagent was used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections were performed in 35 mm Mattek dishes using 4 μl of TransIT-BrCa Transfection Reagent to deliver 2 μg of DNA (2:1 reagent:DNA ratio). Images were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

Figure 3. TransIT-BrCa Transfection Reagent Yields High Efficiency Plasmid DNA Transfection. TransIT-BrCa Transfection Reagent was used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections were performed in 24-well plates using 1-1.5 μl of TransIT-BrCa Transfection Reagent to deliver 0.5 μg of DNA (2:1 and 3:1, reagent:DNA ratio). Triplicate wells were assayed 48 hours post-transfection on a Guava HT easyCyte flow cytometer.

Figure 4. Activation of Estrogen Receptor Pathway is detected in MCF-7 Cells Transfected with TransIT-BrCa Transfection Reagent. MCF-7 (estrogen receptor positive) and MDA-MB-231 (estrogen receptor negative) breast cancer cells were maintained in complete media containing charcoal stripped FBS for 3 days prior to transfection. TransIT-BrCa Transfection Reagent was used to deliver an ERE-luciferase reporter plasmid or negative control (SA Biosciences) at a 2:1 reagent-to-DNA ratio. Transfections were performed in 96-well plates using 0.1 μg of plasmid DNA per well. Twenty four hours post-transfection, cells were treated with varying levels of 17-estradiol (E2) for 6 hours. Firefly and Renilla luciferase expression was measured at 30 hours post-transfection using a standard assay. Promoter activity is represented as the ratio of firefly to luciferase relative light units (RLU) using the Renilla luciferase for normalization. Error bars represent the standard deviation of triplicate wells.

Keyword : MIR5504, MIR5500, MIR5505, MIR5506, v5504,v5500,v5505,v5506
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