´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.

TransIT-PRO¢ç Transfection Reagent & Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 5700
TransIT-PRO¢ç Transfection Kit (includes PRO Boost Reagent)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml
°¡°Ý¹®ÀÇ   transit_pro_transfection_kit.pdf
Mirus
MIR 5760
TransIT-PRO¢ç Transfection Kit (includes PRO Boost Reagent)
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 5740
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 ml
°¡°Ý¹®ÀÇ   transit-pro-transfection-reagent-protocol.pdf
Mirus
MIR 5750
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
°¡°Ý¹®ÀÇ  



Á¦Ç°¼³¸í
TransIT-PRO Transfection Kit (includes Pro Boost reagent) Àº ºÎÀ¯ ¼¼Æ÷ÀÎ 293 cell°ú CHO cell¿¡¼­ ´Ü¹éÁúÀ» »ý»êÇϵµ·Ï °³¹ßµÈ DNA transfection ½Ã¾à°ú boost ½Ã¾àÀ¸·Î ±¸¼ºµÇ¾î
ÀÖ´Ù. PRO Boost Reagent´Â ¹èÁö¿¡ µû¶ó ¼±ÅÃÀûÀ¸·Î »ç¿ëÇÏ´Â ½Ã¾àÀ¸·Î ¹ßÇöÀ» Áõ°¡½ÃÅ°´Â ¿ªÇÒÀ» ÇÑ´Ù. TransIT-PRO Transfection Reagent¿Í PRO Boost Reagent´Â animal-origin free components·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç ´Ù¾çÇÑ ¹èÁö¿¡ Àû¿ë °¡´ÉÇÏ´Ù. TransIT-PRO Transfection KitÀº transfection ÈÄÀÇ ¹èÁö ±³È¯ÀÌ ÇÊ¿äÇÏÁö ¾Ê°í transient transfectionÀ̳ª stable transfection ¸ðµÎ¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
  • Suspension CHO¿Í 293 cell ¿ë transfection reagent
  • ´Ù¾çÇÑ CHO ¹èÁö Á¶¼º¿¡¼­ ȣȯ¼º ¿ì¼ö
  • ´Ù¾çÇÑ culture volume (4-400 §¢)¿¡ Àû¿ë °¡´É
TransIT-PRO¢ç Transfection Kit (includes PRO Boost Reagent)

Code

TransIT-PRO¢ç Transfection Reagent ¿ë·®

PRO Boost Reagent
¿ë·®

MIR5700

1 ml

1.5 ml

MIR 5760

10 ml

15 ml


¼Â¾÷ÀÌ °£ÆíÇÑ ´ë·® ´Ü¹éÁú »ý»ê system
CHOgro¢ç Expression System ÀÚ¼¼È÷º¸±â
Transfection ÈÄ ½Ã°£°æ°ú¿¡ µû¸¥ ¹ßÇöÀ² ºñ±³

Figure 1. Achieve high antibody titers using the TransIT-PRO¢ç Transfection Kit. Human IgG1 was produced by transient transfection using TransIT-PRO and PRO Boost Reagent, 25kDa linear PEI or FreeStyle¢â Max transfection reagents according to the manufacturers or published protocol. Transfections were performed using 1§¶ plasmid DNA per milliliter of culture and 0.5 x 106 cells/§¢ at the time of transfection. FreeStyle¢â CHO-S cells were cultured in 20 §¢ of FreeStyle¢â CHO Expression medium in 125 §¢ shake flasks. Day 3, 5 and 7 supernatants were clarified and analyzed using a human IgG-Fc sandwich ELISA.
°í°´ÀÌ Á÷Á¢ °æÇèÇÑ TransIT-PRO¢ç ³ôÀº ¹ßÇö

Figure 2. Evaluation of EGFP-C3 protein expression level upon transient transfection with different reagents. EGFP-C3 protein expression per viable cell at 37 ¡ÆC & 32 ¡ÆC.
[ÀÎ¿ë ³í¹®. Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells. Sou S, et al. ABB, 2013 Dec. Vol. 4.]
°ü·Ã Á¦Ç°
Endotoxin Á¦°Å¸¦ ÅëÇÑ ´Ü¹éÁú ¹ßÇö Çâ»ó MiraCLEAN¢ç Endotoxin Removal Kit ÀÚ¼¼È÷º¸±â
Transfection È¿À² È®Àοë Label IT¢ç Plasmid Delivery Control, Cy¢ç 3 ÀÚ¼¼È÷º¸±â

Keyword : V5700,MIR5700,GMP,V5760,MIR5760

È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
>
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
TransIT-VirusGEN¢ç Transfection Reagent
>
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
TransIT¢ç-Lenti Transfection Reagent
>
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
>
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
>
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit