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Large RNA & CRISPR guide RNA¿ëtransfection ½Ã¾à

TransIT¢ç-mRNA Transfection Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 2225
TransIT-mRNA Transfection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.4 ml
°¡°Ý¹®ÀÇ   ml036_transit_mrna_transfection_kit.pdf
Mirus
MIR 2250
TransIT-mRNA Transfection Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1.0 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 2255
TransIT-mRNA Transfection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.0 ml¡¿5
°¡°Ý¹®ÀÇ  
Mirus
MIR 2256
TransIT-mRNA Transfection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.0 ml¡¿10
°¡°Ý¹®ÀÇ  

Á¦Ç°¼³¸í
RNA delivery´Â Á÷Á¢ ¼¼Æ÷Áú¿¡ RNA¸¦ Àü´ÞÇØ ´Ü¹éÁúÀ» ¹ßÇö½ÃÅ°±â ¶§¹®¿¡ ´Ù¾çÇÑ transcriptional regulationÀ» ÇÇÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ´Ù¾çÇÑ Å¸ÀÔÀÇ cell¿¡ ³·Àº µ¶¼ºÀ¸·Î RNA¸¦ transfectionÇϱâ À§ÇØ °³¹ßÇÑ ½Ã¾àÀ¸·Î mRNA ȤÀº viral RNA µî ´Ù¾çÇÑ Å©±âÀÇ RNA ºÐÀÚ (2~10 kb)¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù. µû¶ó¼­ ÀϽÃÀûÀÎ ´Ü¹éÁú ¹ßÇö, ¹ÙÀÌ·¯½º »ý»ê ¹× º¹Á¦ ¿¬±¸ µî ´Ù¾çÇÑ ºÐ¾ß¿¡ »ç¿ëµÉ ¼ö ÀÖ´Ù.
³»¿ë
(#MIR 2250 ±âÁØ; 1.0 §¢)
±¸¼ºÇ°

¿ë·®

TransIT¢ç-mRNA Reagent

1 x 1.0 §¢

mRNA Boost Reagent

1 x 1.0 §¢

º¸Á¸: 4 ¡É
°ü·ÃÁ¦Ç°
- Çѹø¿¡ 200 §¶ mRNA ÇÕ¼º, °í¼öÀ² in vitro transcription: Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)
- IVT¿ë Plasmid templateÀ» °£´ÜÇÏ°Ô Á¦ÀÛÇÏ´Â Kit: Cloning Kit for mRNA Template (Code 6143)
- Capping enzyme, uncapped RNA -> cap-0 RNA: Vaccinia Capping Enzyme (Code 2460A)
- Capping enzyme, cap-0 RNA -> cap-1 RNA: mRNA Cap 2-O-Methyltransferase (Code 2470A)
- Æ÷À¯µ¿¹° ¼¼Æ÷¿ë Electroporation ½Ã¾à: Ingenio¢ç Electroporation Solution (Code MIR 50111)
½ÇÇè µ¥ÀÌÅÍ

±×¸² 1. Ÿ»ç ´ëºñ ³ôÀº µµÀÔÈ¿À²°ú ³·Àº µ¶¼ºÀ» º¸ÀÌ´Â TransIT¢ç-mRNA Transfection Kit. TransIT¢ç-mRNA Transfection Kit, Lipofectamine¢ç RNAiMAX (Life Technologies), Stemfect¢â RNA Transfection Kit (Stemgent) ¼¼°¡Áö ½Ã¾àÀ» »ç¿ëÇØ 14ÀÏ°£ µ¿ÀÏÇÑ populationÀÇ BJ fibroblasts¿¡ RNA¸¦ ¸ÅÀÏ transfectionÇÏ¿´´Ù (capping ¹× polyadenylationÀÌ ¿Ï·áµÈ EGFP mRNA - pseudouridine°ú 5mC modified bases (Trilink Biotechnologies, Inc.) »ç¿ë). ½ÇÇèÀº 12-well plate¿¡¼­ 1 §¶ RNA¸¦ °¢°¢ ½Ã¾àÀÇ ÃÖÀû ºñÀ²·Î transfectionÇÏ¿´°í, transfection ÈÄ ¸ÅÀÏ °°Àº À§Ä¡¿¡¼­ GFP¿Í À§»óÂ÷ À̹ÌÁö¸¦ ÃÔ¿µÇÏ¿´´Ù. Transfection È¿À²Àº 14ȸÀÇ daily transfection ÈÄ flow cytometry (Guava¢ç easyCyte¢â 5HT)¸¦ »ç¿ëÇØ ÃøÁ¤ÇÏ¿´°í (blue bar), ¼¼Æ÷ »ýÁ¸À²À» flow cytometry¸¦ ÅëÇØ cell count·Î ÃøÁ¤ÇÏ¿´´Ù (black line grey bar). error bar´Â 3 ¹Ýº¹ ½ÇÇèÇÑ wellÀÇ Ç¥ÁØÆíÂ÷¸¦ ÀǹÌÇÑ´Ù. ±× °á°ú TransIT¢ç-mRNA Transfection Kit´Â 14ÀÏ°£ÀÇ Àå±â°£ÀÇ ¹Ýº¹µÇ´Â ½ÇÇè¿¡¼­ ³ôÀº µµÀÔÈ¿À²°ú ³·Àº µ¶¼ºÀ¸·Î Ÿ»ç ´ëºñ ³ôÀº È¿À²À» º¸¿´´Ù.


±×¸² 2. Luciferase mRNA¸¦ ´Ù¾çÇÑ Cell¿¡ µµÀÔ ÈÄ È®ÀÎÇÑ high level luciferase expression °á°ú. 12-well plate¿¡ ¹è¾çµÈ ¼¼Æ÷¿¡ capping ¹× polyadenylationÀÌ ¿Ï·áµÈ luciferase encoding mRNA¸¦ TransIT¢ç-mRNA Transfection Kit·Î transfection ÇÑ °á°úÀÌ´Ù. Transfection ÈÄ ¾à 18½Ã°£ µÚ ¼¼Æ÷¸¦ ȸ¼öÇØ well´ç total luciferase activity¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú ´Ù¾çÇÑ cell¿¡¼­ ³ôÀº È¿À²·Î À¯ÀüÀÚ¸¦ ¹ßÇö½Ãų ¼ö ÀÖ¾ú´Ù.


±×¸² 3. CHO-K1 Cell¿¡ LacZ encoding mRNA¸¦ transfectionÇÑ °á°ú. CHO-K1 ¼¼Æ÷¿¡ (A) mock °ú (B) capping ¹× polyadenylationÀÌ ¿Ï·áµÈ LacZ encoding mRNA¸¦ °¢°¢ TransIT¢ç-mRNA Transfection Kit·Î transfection ÇÑ °á°úÀÌ´Ù. Transfection ÈÄ ¾à 18 ½Ã°£ µÚ ÀÌ ¼¼Æ÷µéÀ» Beta-gal Staining Kit·Î ¿°»öÇØ LacZ°¡ transfectionµÈ ¼¼Æ÷¸¦ È®ÀÎÇß´Ù. ±× °á°ú LacZ mRNA¸¦ µµÀÔÇÑ cell¸¸ È¿°úÀûÀ¸·Î ½ºÅ©¸®´× ÇÒ ¼ö ÀÖ¾ú´Ù.

Keyword : V2225,MIR2225,V2250,MIR2250,V2255T,MIR2255,V2256,MIR2256

µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
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Large RNA & CRISPR guide RNA¿ëtransfection ..
TransIT¢ç-mRNA Transfection Kit
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mRNA, sgRNA, siRNAµî ´Ù¾çÇÑ RNA transfection..
Xfect¢â RNA Transfection Reagent