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Transfection ½Ã¾à
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µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
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Trans
IT¢ç-mRNA Transfection Kit
Large RNA & CRISPR guide RNA¿ëtransfection ½Ã¾à
Trans
IT¢ç-mRNA Transfection Kit
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Á¦Ç°¸í
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(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 2225
Trans
IT-mRNA Transfection Kit
0.4 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 2250
Trans
IT-mRNA Transfection Kit
1.0 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 2255
Trans
IT-mRNA Transfection Kit
1.0 ml¡¿5
°¡°Ý¹®ÀÇ
Mirus
MIR 2256
Trans
IT-mRNA Transfection Kit
1.0 ml¡¿10
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Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
³·Àº ¼¼Æ÷ µ¶¼º
- ÀÏÁ¤ÇÑ cell density¸¦ À¯ÁöÇÏ°í ÀçÇö¼º ³ôÀº ½ÇÇè °¡´É
°íÈ¿À² transfection
- Large cell population¿¡¼ RNAÀÇ °íÈ¿À² Àü´Þ·Î ³ôÀº ½ÇÇè ¼º°øÀ²
Serum
ȣȯ¼º
- ¹èÁö ±³È¯ÀÌ ÇÊ¿ä ¾ø°í ¼¼Æ÷ÀÇ ³ôÀº È°¼º À¯Áö
´Ù¾çÇÑ Å©±âÀÇ RNA Àü´Þ
- ¹ÙÀÌ·¯½º »ý»ê, ´Ü¹éÁú ¹ßÇö, À¯ÀüÀÚ ÆíÁý (CRISPR) µî ´Ù¾çÇÑ application
- Stem cell application
º¸·¯°¡±â
- CRISPR/Cas9 À¯ÀüÀÚ ÆíÁý application
º¸·¯°¡±â
(
CRISPR mRNA Delivery Protocol (PDF)
)
- °í¿ª°¡ virus »ý»ê application
º¸·¯°¡±â
-
Transfection of Pseudouridine and 5-Methylcytidine Modified Messenger RNA for iPS Cell Reprogramming (PDF)
-
In Vitro
Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection.
Jani, B., Fuchs, R. J. Vis. Exp. (61), e3702.
Á¦Ç°¼³¸í
RNA delivery´Â Á÷Á¢ ¼¼Æ÷Áú¿¡ RNA¸¦ Àü´ÞÇØ ´Ü¹éÁúÀ» ¹ßÇö½ÃÅ°±â ¶§¹®¿¡ ´Ù¾çÇÑ transcriptional regulationÀ» ÇÇÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ´Ù¾çÇÑ Å¸ÀÔÀÇ cell¿¡ ³·Àº µ¶¼ºÀ¸·Î RNA¸¦ transfectionÇϱâ À§ÇØ °³¹ßÇÑ ½Ã¾àÀ¸·Î mRNA ȤÀº viral RNA µî ´Ù¾çÇÑ Å©±âÀÇ RNA ºÐÀÚ (2~10 kb)¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù. µû¶ó¼ ÀϽÃÀûÀÎ ´Ü¹éÁú ¹ßÇö, ¹ÙÀÌ·¯½º »ý»ê ¹× º¹Á¦ ¿¬±¸ µî ´Ù¾çÇÑ ºÐ¾ß¿¡ »ç¿ëµÉ ¼ö ÀÖ´Ù.
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(#MIR 2250 ±âÁØ; 1.0 §¢)
±¸¼ºÇ°
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Trans
IT
¢ç
-mRNA Reagent
1 x 1.0 §¢
mRNA Boost Reagent
1 x 1.0 §¢
º¸Á¸: 4 ¡É
°ü·ÃÁ¦Ç°
- Çѹø¿¡ 200 §¶ mRNA ÇÕ¼º, °í¼öÀ²
in vitro
transcription:
Takara IVTpro¢â T7 mRNA Synthesis Kit (Code 6144)
- IVT¿ë Plasmid templateÀ» °£´ÜÇÏ°Ô Á¦ÀÛÇÏ´Â Kit:
Cloning Kit for mRNA Template (Code 6143)
- Capping enzyme, uncapped RNA -> cap-0 RNA:
Vaccinia Capping Enzyme (Code 2460A)
- Capping enzyme, cap-0 RNA -> cap-1 RNA:
mRNA Cap 2-O-Methyltransferase (Code 2470A)
- Æ÷À¯µ¿¹° ¼¼Æ÷¿ë Electroporation ½Ã¾à:
Ingenio
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Electroporation Solution (Code MIR 50111)
½ÇÇè µ¥ÀÌÅÍ
±×¸² 1. Ÿ»ç ´ëºñ ³ôÀº µµÀÔÈ¿À²°ú ³·Àº µ¶¼ºÀ» º¸ÀÌ´Â
Trans
IT
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-mRNA Transfection Kit.
Trans
IT
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-mRNA Transfection Kit, Lipofectamine
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RNAiMAX (Life Technologies), Stemfect¢â RNA Transfection Kit (Stemgent) ¼¼°¡Áö ½Ã¾àÀ» »ç¿ëÇØ 14ÀÏ°£ µ¿ÀÏÇÑ populationÀÇ BJ fibroblasts¿¡ RNA¸¦ ¸ÅÀÏ transfectionÇÏ¿´´Ù (capping ¹× polyadenylationÀÌ ¿Ï·áµÈ EGFP mRNA - pseudouridine°ú 5mC modified bases (Trilink Biotechnologies, Inc.) »ç¿ë). ½ÇÇèÀº 12-well plate¿¡¼ 1 §¶ RNA¸¦ °¢°¢ ½Ã¾àÀÇ ÃÖÀû ºñÀ²·Î transfectionÇÏ¿´°í, transfection ÈÄ ¸ÅÀÏ °°Àº À§Ä¡¿¡¼ GFP¿Í À§»óÂ÷ À̹ÌÁö¸¦ ÃÔ¿µÇÏ¿´´Ù. Transfection È¿À²Àº 14ȸÀÇ daily transfection ÈÄ flow cytometry (Guava
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easyCyte¢â 5HT)¸¦ »ç¿ëÇØ ÃøÁ¤ÇÏ¿´°í (blue bar), ¼¼Æ÷ »ýÁ¸À²À» flow cytometry¸¦ ÅëÇØ cell count·Î ÃøÁ¤ÇÏ¿´´Ù (black line grey bar). error bar´Â 3 ¹Ýº¹ ½ÇÇèÇÑ wellÀÇ Ç¥ÁØÆíÂ÷¸¦ ÀǹÌÇÑ´Ù. ±× °á°ú
Trans
IT
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-mRNA Transfection Kit´Â 14ÀÏ°£ÀÇ Àå±â°£ÀÇ ¹Ýº¹µÇ´Â ½ÇÇè¿¡¼ ³ôÀº µµÀÔÈ¿À²°ú ³·Àº µ¶¼ºÀ¸·Î Ÿ»ç ´ëºñ ³ôÀº È¿À²À» º¸¿´´Ù.
±×¸² 2. Luciferase mRNA¸¦ ´Ù¾çÇÑ Cell¿¡ µµÀÔ ÈÄ È®ÀÎÇÑ high level luciferase expression °á°ú.
12-well plate¿¡ ¹è¾çµÈ ¼¼Æ÷¿¡ capping ¹× polyadenylationÀÌ ¿Ï·áµÈ luciferase encoding mRNA¸¦
Trans
IT
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-mRNA Transfection Kit·Î transfection ÇÑ °á°úÀÌ´Ù. Transfection ÈÄ ¾à 18½Ã°£ µÚ ¼¼Æ÷¸¦ ȸ¼öÇØ well´ç total luciferase activity¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú ´Ù¾çÇÑ cell¿¡¼ ³ôÀº È¿À²·Î À¯ÀüÀÚ¸¦ ¹ßÇö½Ãų ¼ö ÀÖ¾ú´Ù.
±×¸² 3. CHO-K1 Cell¿¡ LacZ encoding mRNA¸¦ transfectionÇÑ °á°ú.
CHO-K1 ¼¼Æ÷¿¡
(A) mock
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(B) capping ¹× polyadenylationÀÌ ¿Ï·áµÈ LacZ encoding mRNA
¸¦ °¢°¢
Trans
IT
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-mRNA Transfection Kit·Î transfection ÇÑ °á°úÀÌ´Ù. Transfection ÈÄ ¾à 18 ½Ã°£ µÚ ÀÌ ¼¼Æ÷µéÀ» Beta-gal Staining Kit·Î ¿°»öÇØ LacZ°¡ transfectionµÈ ¼¼Æ÷¸¦ È®ÀÎÇß´Ù. ±× °á°ú LacZ mRNA¸¦ µµÀÔÇÑ cell¸¸ È¿°úÀûÀ¸·Î ½ºÅ©¸®´× ÇÒ ¼ö ÀÖ¾ú´Ù.
Keyword : V2225,MIR2225,V2250,MIR2250,V2255T,MIR2255,V2256,MIR2256
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
Large RNA & CRISPR guide RNA¿ëtransfection ..
Trans
IT¢ç-mRNA Transfection Kit
Trans
IT¢ç-Oligo Transfection Reagent
mRNA, sgRNA, siRNAµî ´Ù¾çÇÑ RNA transfection..
Xfect¢â RNA Transfection Reagent
Xfect¢â Protein Transfection Reagent