Cloning Kit ¼±Åð¡À̵å
[½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit]
½Å¼Ó, °£Æí, °íÈ¿À² ligation
Blunt end ligation
Long insert (>10 kb ligation)
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insert ligation
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insert ligation
; PrimeSTAR¢ç µî high fidelity È¿¼Ò¸¦
ÀÌ¿ëÇÑ °æ¿ì
½Å¼Ó, °íÈ¿À² cloning
In-Fusion¢ç Snap Assembly Master Mix
- »óµ¿¼¿À» ÀçÁ¶ÇÕÇÏ¿© ´Ü 15ºÐ¸¸¿¡ cloning ¿Ï·á
- »ç¿ëÇÒ vectorÀÇ ¾ç ¸»´Ü ¼¿À» ÀÌ¿ëÇϹǷΠ¿°±âÃß°¡ ºÒÇÊ¿ä
- Multiple fragment, large insert cloning¿¡µµ ÃÖÀû
Multiple fragment cloning
Mutagenesis
[Ligation, dephosphorylation ¿¡ »ç¿ëÇÏ´Â È¿¼Ò]
[In-Fusion CloningÀ» À§ÇÑ ¿Â¶óÀÎ Åø]