Low input |
1 ng-2 §¶ ¼öÁØÀÇ ¾ÆÁÖ ÀûÀº ¾çÀÇ RNA sample¿¡¼ NGS library Á¦ÀÛ |
Small RNA-Seq |
150 nt ÀÌÇÏÀÇ small RNA (miRNA, siRNA, piRNA, snoRNA, snRNA ¿Ü) ºÐ¼® |
Fast and Simple |
3½Ã°£ À̳» Illumina NGS library Á¦ÀÛ |
SMART-Seq |
µ¥ÀÌÅÍ ÆíÇ⼺À» ÃÖ¼ÒÈ (Gel-free, Ligation-free protocol) |
2 kits-in-1 |
miRNA Á¤·®¿¡ ÇÊ¿äÇÑ ¸ðµç Á¦Ç° Æ÷ÇÔ (cDNA synthesis kit + qPCR premix) |
Simple |
½±°í ºü¸¥ cDNA ÇÕ¼º°ú qPCR Á¤·® |
Sensitive |
¹Î°¨ÇÑ °ËÃâ °¨µµ (50 copies ¼öÁرîÁö °ËÃâ) |
Flexible |
ÇÕ¼ºÇÑ cDNA´Â miRNA »Ó¸¸ ¾Æ´Ï¶ó mRNA ºÐ¼®¿¡ ¸ðµÎ ÀÌ¿ë °¡´É |
- ÇϳªÀÇ cDNA·Î miRNA¿Í miRNA targetÀ» À¯ÀǼº ÀÖ°Ô ºÐ¼® |
Doxycycline (Tetracycline)À» ÀÌ¿ëÇÏ¿© Á¤¹ÐÇÏ°Ô miRNA ¹ßÇö Á¶Àý |
|
Tet-on¢ç Advanced ±â¼úÀÌ °áÇյǾî Doxycycline ó¸® ½Ã¿¡¸¸ miRNA ¹ßÇö |
|
³·Àº basal expression°ú ³ôÀº fold induction |
|
Çü±¤´Ü¹éÁúÀÌ ÇÔ²² ¹ßÇöµÇ¾î miRNA ¹ßÇö·® È®ÀÎ °¡´É (Red or Green) |
miRNA ½ÇÇè ºÐ·ù |
Á¦Ç°¸í |
Code |
¿ë·® |
Small RNA Sequencing |
635029 |
12 ȸ |
|
635030 |
48 ȸ |
||
635031 |
96 ȸ |
||
RT-PCR |
638314 |
200 ȸ |
|
638316 |
600 ȸ |
||
638313 |
20 ȸ |
||
638315 |
60 ȸ |
||
miRNA |
631118 |
1 set |
|
631120 |
1 set |
||
631119 |
20 §¶ |
||
631121 |
20 §¶ |
||
Cloning |
638947 |
10 ȸ |
|
RR065 |
10 ȸ |
Broad Spectrum Transfection Reagent for Gene Silencing | ||