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    MicroRNA ¿¬±¸


MicroRNA (miRNA) ¿¬±¸¿ë Á¦Ç° °¡À̵å

MicroRNAÀÇ ÀÌÇØ
1930³â Victor Ambros ¿¬±¸ÁøÀÇ C.elegans lin-14 ¿¬±¸°úÁ¤¿¡¼­ ¹ß°ßµÈ microRNA (miRNA)´Â ¾à 22°³ nucleotide·Î ÀÌ·ç¾îÁø non-coding RNA·Î½á, À¯ÀüÀÚÀÇ Àü»ç ÈÄ ´Ü°è¿¡¼­ ´Ü¹éÁú ¹ßÇöÀ» Á¶ÀýÇϸç Æ÷À¯·ùÀÇ °æ¿ì À¯ÀüÀÚÀÇ 60% Á¤µµ°¡ miRNA¿¡ ÀÇÇØ Àü»ç ÈÄ ´Ü°è¿¡¼­ ´Ü¹éÁú ¹ßÇöÀÌ Á¶ÀýµÇ´Â °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù. miRNA´Â ¾Ï, ½ÉÀåÁúȯ, ½Å°æ°ü·Ã µîÀÇ Áúº´¿¡ ¿¬°üÀÌ ÀÖ´Â °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Ù.

1. miRNA Screening & Profiling
NGS¸¦ ÀÌ¿ëÇÑ miRNA ´ë·® °ËÃâ ¹× ºÐ¼®
¤ýSMARTer¢ç smRNA-Seq Kit for Illumina¢ç (Code 635029)

Low input

1 ng-2 §¶ ¼öÁØÀÇ ¾ÆÁÖ ÀûÀº ¾çÀÇ RNA sample¿¡¼­ NGS library Á¦ÀÛ

Small RNA-Seq

150 nt ÀÌÇÏÀÇ small RNA (miRNA, siRNA, piRNA, snoRNA, snRNA ¿Ü) ºÐ¼®

Fast and Simple

3½Ã°£ À̳» Illumina NGS library Á¦ÀÛ

SMART-Seq

µ¥ÀÌÅÍ ÆíÇ⼺À» ÃÖ¼ÒÈ­ (Gel-free, Ligation-free protocol)


2. miRNA °ËÃâ°ú Á¤·®
ÇÕ¸®ÀûÀÎ Two-in-One qRT-PCR Kit (miRNA Á¤·®Å°Æ®)
¤ýMir-X¢â miRNA qRT-PCR TB Green¢ç Kit (Code 638314/638316)

2 kits-in-1

miRNA Á¤·®¿¡ ÇÊ¿äÇÑ ¸ðµç Á¦Ç° Æ÷ÇÔ (cDNA synthesis kit + qPCR premix)

Simple

½±°í ºü¸¥ cDNA ÇÕ¼º°ú qPCR Á¤·®

Sensitive

¹Î°¨ÇÑ °ËÃâ °¨µµ (50 copies ¼öÁرîÁö °ËÃâ)

Flexible

ÇÕ¼ºÇÑ cDNA´Â miRNA »Ó¸¸ ¾Æ´Ï¶ó mRNA ºÐ¼®¿¡ ¸ðµÎ ÀÌ¿ë °¡´É

- ÇϳªÀÇ cDNA·Î miRNA¿Í miRNA targetÀ» À¯ÀǼº ÀÖ°Ô ºÐ¼®


3. miRNA ±â´É ºÐ¼®
Tet-inducible expression systemÀ» ÀÌ¿ëÇÑ miRNA À¯µµ ¹ßÇö
¤ýMir-X¢â Inducible miRNA System (Red) (Code 631118)
¤ýMir-X¢â Inducible miRNA System (Green) (Code 631120)

Doxycycline (Tetracycline)À» ÀÌ¿ëÇÏ¿© Á¤¹ÐÇÏ°Ô miRNA ¹ßÇö Á¶Àý

Tet-on¢ç Advanced ±â¼úÀÌ °áÇյǾî Doxycycline ó¸® ½Ã¿¡¸¸ miRNA ¹ßÇö

³·Àº basal expression°ú ³ôÀº fold induction

Çü±¤´Ü¹éÁúÀÌ ÇÔ²² ¹ßÇöµÇ¾î miRNA ¹ßÇö·® È®ÀÎ °¡´É (Red or Green)


4. °ü·Ã Á¦Ç° ¸®½ºÆ®

miRNA ½ÇÇè ºÐ·ù

Á¦Ç°¸í

Code

¿ë·®

Small RNA Sequencing

SMARTer¢ç smRNA-Seq Kit for Illumina¢ç BEST

635029

12 ȸ

635030

48 ȸ

635031

96 ȸ

RT-PCR
Quantification

Mir-X¢â miRNA qRT-PCR TB Green¢ç Kit

638314

200 ȸ

638316

600 ȸ

Mir-X¢â miRNA First-Strand Synthesis Kit

638313

20 ȸ

638315

60 ȸ

miRNA
Expression

Mir-X¢â Inducible miRNA System (Red)

631118

1 set

Mir-X¢â Inducible miRNA System (Green)

631120

1 set

pmRi-mCherry Vector

631119

20 §¶

pmRi-ZsGreen1 Vector

631121

20 §¶

Cloning

In-Fusion¢ç Snap Assembly Master Mix

638947

10 ȸ

small RNA Cloning Kit

RR065

10 ȸ



Broad Spectrum Transfection Reagent for Gene Silencing

TransIT-X2¢ç
Dynamic Delivery System

TransIT-TKO¢ç
Transfection Reagent

TransIT-siQUEST¢ç
Transfection Reagent