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NGS °ü·Ã
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Small RNA-Seq
> Small RNA-Seq Kit
Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ºÐ¼®À» À§ÇÑ NGS Library Á¦ÀÛ
Small RNA-Seq Kit
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Clontech
635029
SMARTer¢ç smRNA-Seq Kit for Illumina¢ç
12ȸ
1,122,400¿ø
1,403,000¿ø
05.02 ~ 06.30
Clontech
635030
SMARTer¢ç smRNA-Seq Kit for Illumina¢ç
48 ȸ
4,077,600¿ø
5,097,000¿ø
05.02 ~ 06.30
Clontech
635031
SMARTer¢ç smRNA-Seq Kit for Illumina¢ç
96 ȸ
7,321,600¿ø
9,152,000¿ø
05.02 ~ 06.30
Product index
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
[FAQ] RNA-seq
Cancer ¿¬±¸ °¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
SMARTer RNA-seqÀÇ HOT ..
mRNA-Seq (Single cell)
[Cancer Research] Canc..
Single cell-seq °¡À̵å
Single cell SMART-seq..
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
[Cancer Research] Canc..
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Cancer Research] Immu..
[Version 2] Human TCR ..
Human TCR a/b Profilin..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
Single cell-seq °¡À̵å
[FAQ] DNA-Seq
[Àû¿ë] Overview_ThruPL..
[Cancer Research] Canc..
DNA-seqÀÇ ´Ù¾çÇÑ ÃֽŠ..
ChIP-Seq / Meth-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[³í¹®] ThruPLEX¢ç for ..
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
[Cancer Research] Canc..
Single cell-seq °¡À̵å
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
[Cancer Research] HLA ..
[³í¹®] PicoPLEX¢ç ±â¼ú..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
[FAQ] Library index
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Citation
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) SMARTer¢ç Str..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
(Legacy) ThruPLEX¢ç Ta..
Supplemental material for the SMARTer¢ç smRNA-seq Kit for Illumina¢ç (Click)
Analysis of diverse small RNA species:
small RNA (15 - 150 nt) sequencing library Á¦ÀÛ
Flexible sample inputs:
total RNA ¶Ç´Â enriched small RNA 1 ng - 2ug
Illumina¢ç-ready sequencing libraries:
Illumina¢ç adaptor¿Í index¸¦ º°µµÀÇ Ligation °úÁ¤¾øÀÌ SMARTer¢ç ±â¼ú·Î ºÎÂø
Quick, single-tube workflow:
3½Ã°£ À̳»¿¡ sequencing library Á¦ÀÛ °¡´É
Á¦Ç°¼³¸í
Small non-coding RNAs (smRNAs) ¹ß°ß°ú ºÐ¼® ¿¬±¸´Â À¯ÀüÀÚ ¹ßÇöÀÇ Á¶Àý ±âÀÛÀ» ¿¬±¸ÇÏ´Â µ¥ ÀÖ¾î Å« ±â¿©¸¦ ÇØ¿Ô´Ù. ÁÖ·Î ¾Ë·ÁÁø small RNAs´Â miRNAs, siRNAs, piRNAs, snoRNAs·Î½á 20 - 30 ntÀÇ Å©±â¸¦ °¡Áö°í ÀÖ´Ù. SMARTer¢ç smRNA-Seq Kit for Illumina¢ç¸¦ ÀÌ¿ëÇÏ¸é ¼Ò·®ÀÇ small RNAs (low-input amounts)ºÎÅÍ °íǰÁúÀÇ Illumina¢ç-ready sequencing library¸¦ Á¦ÀÛ ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº 1ng - 2ugÀÇ total RNA ¶Ç´Â enriched small RNA sample·ÎºÎÅÍ SMART-Seq¢ç v4 ±â¼úÀ» ÀÌ¿ëÇÏ¿© Illumina¢ç sequencing library¸¦ Á¦ÀÛÇÑ´Ù. SMART technology (
S
witching
M
echanism
a
t 5¡¯ End of
R
NA
T
emplate)¿Í Locked Nucleic Acids (LNAs) ±â¼úÀÌ °áÇÕµÈ SMART-Seq¢ç v4 ±â¼úÀ» ÀÌ¿ëÇÔÀ¸·Î½á, ¼Ò·®ÀÇ small RNAs·ÎºÎÅÍ ÆíÇ⼺ÀÌ ¾ÆÁÖ ÀûÀº (minimal bias) NGS library¸¦ Á¦ÀÛ °¡´ÉÇÏ´Ù.
±×¸² 1. Schematic of technology used by the SMARTer¢ç smRNA-Seq Kit for Illumina¢ç
. SMART technology is used in a ligation-free workflow to generate sequencing libraries for Illumina platforms
±×¸² 2. Demonstrating the accuracy of the SMARTer approach for small RNA-seq.
Sequencing libraries were generated from an equimolar pool of 963 synthetic miRNAs (miRXplore Universal Reference) using the SMARTer¢ç smRNA-Seq Kit for Illumina¢ç (1 ng input; purple), or a small RNA-seq kit from a different vendor (Competitor N) employing an adapter ligation method (100 ng input; blue). Following sequencing, mapping, and counting of reads, miRNA expression levels (Y axis, log scale) were normalized, resulting in an expected expression level equal to 1 for each miRNA, and a 2-fold cutoff was assigned both above and below the expected expression level (indicated by two horizontal lines). For visualization purposes, miRNAs are ranked along the X axis in order of expression level.
±×¸² 3. Reproducibility of SMARTer¢ç small RNA-seq data.
Sequencing libraries were generated in parallel from the indicated input amounts of human brain total RNA using the SMARTer smRNA-Seq Kit for Illumina, and size selected using the BluePippin system. Following sequencing, data processing, and mapping, expression levels of miRNAs identified for each library were quantified and plotted on correlation diagrams. Panel A. Correlation of miRNA expression levels for experimental replicates involving 1 ng inputs. Panel B. Correlation of miRNA expression levels for 2 ¥ìg vs. 1 ng inputs.
Application
Illumina-ready sequencing library preparation, specifically for small RNAs ranging in size from 15-150 nt
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
SMARTer¢ç smRNA-Seq Kit for Illumina ¢ç (Code 635029)
SMARTer smRNA-Seq Kit for Illumina Components
Indexing Primer Set HT for Illumina - 12
SeqAmp¢â DNA Polymerase
NucleoSpin Gel and PCR Clean-Up
Keyword : miRNA,microRNA,siRNA,smRNA,small RNA,piRNA,biomarker,638313,638314,638315,638316,exosome,EV,NGS,Next generation sequencing,SMART,smart-seq,Cancer,Cancer research,¾Ï¿¬±¸
Small RNA-Seq
[Cancer Research] Cancer biomarker discovery
Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ..
Small RNA-Seq Kit