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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > [New](+UMI) Pico input stranded RNA-Seq Kit
(with UMI) FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò·® low quality RNA¿¡¼­ ÀçÇö¼º ³ôÀº °á°ú

[New](+UMI) Pico input stranded RNA-Seq Kit

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Clontech
634354
SMART-Seq¢ç Total RNA Pico Input with UMIs (ZapR¢â Mammalian)
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24 ȸ (Package)
3,385,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x348187
Clontech
634355
SMART-Seq¢ç Total RNA Pico Input with UMIs (ZapR¢â Mammalian)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ (Package)
8,593,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634356
SMART-Seq¢ç Total RNA Pico Input with UMIs (ZapR¢â Mammalian)
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384 ȸ (634355x4)
30,934,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

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º» Á¦Ç°Àº ±âÁ¸ SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Code 634485, 634486, 634487, 634488)ÀÇ ¸®´º¾ó Á¦Ç°ÀÔ´Ï´Ù.
ÀϺΠ±¸¼ºÇ°ÀÇ ¾÷±×·¹À̵å·Î ¾Æ·¡¿Í °°ÀÌ ¼º´ÉÀÌ °³¼±µÇ¾ú½À´Ï´Ù.
i. rRNA Á¦°Å ½Ã¾àÀÎ ZapR¢âÀÇ ¼º´ÉÀÌ Çâ»óµÇ¾î ¿øÄ¡ ¾Ê´Â read¸¦ ´õ¿í È¿°úÀûÀ¸·Î Á¦°ÅÇÕ´Ï´Ù.
ii. ÇÊ¿äÇÑ index¸¦ ¼±ÅÃÇÏ¿© Àû¿ëÇÒ ¼ö ÀÖµµ·Ï Á¦Ç°±¸¼º¿¡¼­ index¸¦ Á¦¿ÜÇÏ¿´½À´Ï´Ù. ÇÊ¿ä ½Ã º°µµ ±¸¸ÅÇÒ ¼ö ÀÖ½À´Ï´Ù.
>> ȣȯµÇ´Â Unique Dual Index kits (UDI kits)
ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î ¹®ÀÇ ÁÖ¼¼¿ä.

  • pg ¼öÁØ ±Ø¼Ò·® »ùÇÿ¡ ÃÖÀû - human, mouse, rat À¯·¡ÀÇ 250 pg - 100 ng total RNA ȤÀº 10 - 1,000°³ÀÇ intact cell
  • UMI Àû¿ëÀ¸·Î ºÐ¼® Á¤È®µµ Áõ°¡ - PCR bias, sequencing error µîÀ» º¸Á¤ÇÏ¿© true variant¿Í rare mutationÀÇ Á¤È®ÇÑ ºÐ¼® °¡´É
  • ´Ù¾çÇÑ »ùÇà Àû¿ë - FFPE, LCM, cell-free RNA µî ³·Àº qualityÀÇ RNA¿¡¼­µµ ÀçÇö¼º ³ôÀº °á°ú È®ÀÎ
  • ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝ - Àü °úÁ¤ 6.5½Ã°£¸¸¿¡ ¿Ï·á, buffer Á¶¼ºÀÇ ¾÷±×·¹À̵å·Î library purification ½Ã°£ ´ÜÃà
  • Illumina¢ç Ç÷§Æû¿¡ ȣȯ - Illumina¢ç ºÐ¼®¿ë index¸¦ Àû¿ëÇÏ¿© ÃÖ´ë 384°³ pooling °¡´É
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SMART-Seq¢ç Total RNA Pico Input with UMIs (ZapR¢â Mammalian)Àº picogram input (250 pg - 10 ng total RNA, 10 - 1,000 intact cell)ÀÇ ´Ù¾çÇÑ RNA (high quality, partially degraded, low quality total RNA)¿¡¼­ È¿°úÀûÀ¸·Î Illumina¢ç sequencing library¸¦ Á¦ÀÛÇϱâ À§ÇØ °³¹ßµÇ¾ú´Ù. ±Ø¼Ò·®ÀÇ total RNA»Ó ¾Æ´Ï¶ó FFPE, LCM, cell-free RNA µî RNAÀÇ integrity°¡ ÇöÀúÈ÷ ³·Àº »ùÇÿ¡¼­µµ NGS library Á¦ÀÛÀÌ °¡´ÉÇÏ¸ç ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝÀÌ Æ¯Â¡À¸·Î RT¿Í PCR ´Ü°è¿¡¼­ index¿Í adapter¸¦ Ãß°¡ÇØ strand Á¤º¸ (sense/antisense)¸¦ º¸À¯ÇÏ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â Á¡ÀÌ Æ¯Â¡ÀÌ´Ù. ¶ÇÇÑ RT °úÁ¤¿¡¼­ 8 nucleotide (nt)ÀÇ unique molecular identifier (UMI) ¼­¿­À» Ãß°¡ÇÏ¿© PCR bias¿Í sequencing error¸¦ º¸Á¤ÇÒ ¼ö ÀÖ¾î true variant¿Í rare mutationÀÇ Á¤È®ÇÑ ºÐ¼®ÀÌ °¡´ÉÇÏ´Ù.

ÀÌ Á¦Ç°Àº ´ç»çÀÇ µ¶ÀÚÀûÀÎ SMART (Switching Mechanism at 5¡¯ end of RNA Template) ±â¼ú¿¡ stranded RNA-seqÀ» À§ÇÑ °³¼±»çÇ×À» ´õÇØ ÇÁ·ÎÅäÄÝÀ» °£ÆíÈ­ÇÏ°í sequencing È¿À²À» ³ô¿´´Ù. À̸¦ ÅëÇØ high- ¶Ç´Â low-qualityÀÇ total RNA ¸ðµÎ¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ°í, strand Á¤º¸¸¦ °¡Áø sequencing library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ZapR ±â¼úÀ» Àû¿ëÇÏ¿© rRNAÀ¯·¡ ¼­¿­À» cDNA ÇÕ¼º ÈÄ¿¡ Á¦°ÅÇÒ ¼ö ÀÖÀ½À¸·Î total RNA¸¦ ¹Ù·Î »ç¿ëÇÒ ¼ö ÀÖ¾î ±Ø¼Ò·®ÀÇ RNA ºÐ¼®¿¡ ¸Å¿ì ÀûÇÕÇÏ´Ù.

*±× ¿Ü »ùÇà input ¾ç¿¡ µû¸¥ Ãßõ Á¦Ç°:
- 10 ng - 100 ng total RNA (rRNA Á¦°Å ¹ÌÆ÷ÇÔ) : SMART-Seq¢ç Total RNA Mid Input (Code 635049)
- Mammalian total RNA 100 ng-1 ¥ìg (rRNA Á¦°Å Æ÷ÇÔ) : SMART-Seq¢ç Total RNA High Input (RiboGone¢â Mammalian) (Code 635046)


±×¸² 1. SMART-Seq¢ç Total RNA Pico Input with UMIs (ZapR¢â Mammalian) ÇÁ·Î¼¼½º.
Àû¿ë
  • Strand Á¤º¸¸¦ °¡Áø NGS library Á¦ÀÛ
  • Illumina¢ç Ç÷§Æû RNA-seq
  • ´Ù¾çÇÑ quality¿¡ total mammalian RNA¿¡¼­ coding ¹× non-coding Á¤º¸ È®ÀÎ
    (FFPE »ùÇÿ¡¼­ Á¤Á¦ÇÑ RNA »ç¿ë °¡´É)
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
RNA-Seq ·¯´×¼¾ÅÍ¿¡¼­ ´õ ¸¹Àº µ¥ÀÌÅÍ¿Í FAQ¸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.

Keyword :

Stranded RNA-seq
>
(with UMI) FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò..
[New](+UMI) Pico input stranded RNA-Seq Kit
>
FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò·® low qual..
[New]Pico input stranded RNA-Seq Kit
>
´ÜÀϼ¼Æ÷ (Single cell)¿¡¼­ stranded RNA-Seq ..
[New]Single cell stranded RNA-Seq Kit
>
Mid Input Strand- Specific Total RNA-Seq
[New]Mid Input Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
[New]High Input Stranded RNA-Seq Kit
>
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v3)
>
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v2)
>
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
High Input Stranded RNA-Seq Kit