Á¦Á¶»ç |
Á¦Ç°ÄÚµå |
Á¦Ç°¸í |
¿ë·® |
°¡°Ý (ºÎ°¡¼¼º°µµ) |
ºñ°í |
»ç¿ëÀڸŴº¾ó |
Clontech
|
634836
|
SMARTer¢ç Stranded RNA-Seq Kit
|
12 ȸ
|
1,920,000¿ø
|
|
|
Clontech
|
634837
|
SMARTer¢ç Stranded RNA-Seq Kit
|
24 ȸ
|
3,340,000¿ø
|
|
Clontech
|
634838
|
SMARTer¢ç Stranded RNA-Seq Kit
|
48 ȸ
|
6,020,000¿ø
|
|
Clontech
|
634839
|
SMARTer¢ç Stranded RNA-Seq Kit
|
96 ȸ
|
8,400,000¿ø
|
|
Clontech
|
634862
|
SMARTer¢ç Stranded RNA-Seq Kit HT
|
96ȸ
|
8,400,000¿ø
|
|
,
|
Product index
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SMARTer RNA-Seq °³¿ä
-
mRNA-Seq (Single cell)
-
Stranded RNA-seq
-
Small RNA-Seq
-
Immune-Seq
-
±âŸ RNA-Seq
-
SMARTer DNA-Seq °³¿ä
-
ChIP-Seq / Meth-Seq
-
Low Input DNA-Seq
-
High input DNA-seq
-
Single cell DNA-Seq
-
Whole Genome Amplification..
-
Index Kit
-
NGS Library Á¤·®
-
NGS Sample preparation
-
NGS ºÐ¼®¿ë PCR È¿¼Ò
-
NGS °ü·Ã Àåºñ
-
[Legacy] NGS Products
- SMART¹ýÀ» ÀÌ¿ëÇÑ NGS¿ë Strand-specific NGS Library Á¦ÀÛ
- 100pg-100ngÀÇ RNA (purified Poly A+ RNA ¶Ç´Â rRNA depleted RNA)¿¡¼ Library Á¦ÀÛ
- Illumina¢ç sequencer¿¡ ÀûÇÕÇÑ index¿Í adaptor Àû¿ë
- 4½Ã°£ À̳»¿¡ RNA-seq library Á¦ÀÛ °¡´É
- GC-, AT-rich template¿¡ ÃÖÀûÀÎ PCRÈ¿¼Ò Æ÷ÇÔ
Á¦Ç°¼³¸í
SMARTer¢ç Stranded RNA-Seq Kit´Â 100pg~10ngÀÇ RNA¸¦ ÀÌ¿ëÇÏ¿© Àü»çüÀÇ strand informationÀ» À¯ÁöÇÏ¸é¼ Illumina¢ç sequencing platforms¿¡
Àû¿ëÇÒ ¼ö ÀÖ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ½Ã¾àÀÌ´Ù. º» Á¦Ç°Àº random primer ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© polyA+ RNA¿Í rRNA-removed total RNA·ÎºÎÅÍ NGS¿ë
cDNA library¸¦ Á¦ÀÛÇϸç, mammalian RNA (poly A+ RNA)¿Í ¹ÚÅ׸®¾Æ »ùÇà (non-poly A+ RNA)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
±âÁ¸ÀÇ Clonetch SMARTerÀÇ ¿ø¸®¸¦ ÀϺΠº¯ÇüÇÏ¿© ½ÇÇè °úÁ¤ Áß¿¡, Illumina¢ç index¿Í adaptor¸¦ °áÇÕÇϱ⠶§¹®¿¡ º°µµÀÇ Library preparation kit°¡
ÇÊ¿äÇÏÁö ¾ÊÀ¸¸ç, ºÒ°ú 4½Ã°£ ¸¸¿¡ Illuimina¢ç sequencing¿¡ Àû¿ë °¡´ÉÇÑ indexed library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù.
SMARTer¢ç Stranded RNA-Seq HT (Code 634862)´Â 8Á¾·ùÀÇ Forward primer¿Í 12Á¾·ùÀÇ Reverse primer¸¦ Æ÷ÇÔÇϰí ÀÖ¾î ÃÖ´ë 96°³ÀÇ sampleÀ» ºÐ¼®ÇÒ
¼ö ÀÖ´Â High-throughput systemÀÌ´Ù.
¡Ø SMARTer¢ç Stranded RNA-Seq Kit ¶Ç´Â SMARTer¢ç Stranded RNA-Seq Kit HT Á¦Ç°À» ÀÌ¿ëÇÒ °æ¿ì, ribosomal RNA (rRNA) Á¦°Å
(rRNA depletion)ÀÌ ÇÊ¿äÇϸç, ClontehÀÇ RiboGone¢â-Mammalian (Code 634846)
Á¦Ç°ÀÇ »ç¿ëÀ» ÃßõÇÑ´Ù

±×¸² 1. NGS Work flow for the SMARTer¢ç Stranded RNA-Seq Kit

±×¸² 2. ¼Ò·®ÀÇ RNA (100ng ¶Ç´Â 100pg)·ÎºÎÅÍ ÀçÇö¼º ³ôÀº RNA-Seq µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ÀÖ¾ú´Ù.

±×¸² 3. Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices,
and sequenced on an Illumina¢ç HiSeq¢ç Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands
of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of
RNA-Seq reads mapped to the correct strand.

Ç¥ 1. Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer¢ç Stranded RNA-Seq Kit was able to maintain high
accuracy while detecting ~15,000 genes using strand-specific alignments.
Applications
- Illumina¢ç-specific NGS library production that retains strand information.
- RNA-Seq on Illumina¢ç platforms for both mammalian and prokaryotic samples.
- Coding and non-coding transcript information
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
SMARTer¢ç Stranded RNA-Seq Kit(Code 634836 ¿Ü)
* SMARTer¢ç Stranded RNA-Seq Components
* llumina¢ç Indexing Primer Set
* SeqAmp¢â DNA Polymerase
SMARTer¢ç Stranded RNA-Seq Kit HT (Code 634862)
* SMARTer¢ç Stranded RNA-Seq Components
* Indexing Primer Set HT for Illumina¢ç
* SeqAmp¢â DNA Polymerase
°ü·Ã Á¦Ç°
- Input sample ¾ç¿¡ µû¶ó ¾Æ·¡ÀÇ Á¦Ç°À» ÃßõÇÕ´Ï´Ù.
- ¾Æ·¡ÀÇ Á¦Ç°Àº ¼Ò·®ÀÇ total RNA (10-100ng)·ÎºÎÅÍ rRNA¸¦ Á¦°ÅÇÏ´Â RiboGone¢â-Mammalian (Code 634846)ÀÌ Á¦Ç° ³»¿¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
Keyword : stranded RNA-Seq,Stranded-seq,RNA-Seq,NGS,Illumina