´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Immune-Seq > Mouse TCR a/b Profiling Kit(v1)
Mouse TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ NGS ¼­¿­ ºÐ¼®

Mouse TCR a/b Profiling Kit(v1)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
634403
SMARTer¢ç Mouse TCR a/b Profiling Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
48ȸ
(Package)
6,416,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33051
Clontech
634404
SMARTer¢ç Mouse TCR a/b Profiling Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
(Package)
10,457,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

¡Ø ¾È³»

º» Á¦Ç°Àº SMART-Seq¢ç Mouse TCR (with UMIs) (Code 634814-634816) Á¦Ç°À¸·Î ¾÷±×·¹ÀÌµå µÇ¾ú½À´Ï´Ù.

ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î ¹®ÀÇ ÁÖ¼¼¿ä.
  • Complete V(D)J sequence information - TCR mRNAÀÇ °¡º¯¿µ¿ª(variable regions)ÀÇ Àüü ¼­¿­(Full length) ºÐ¼®
  • TCR-alpha, TCR-beta chainÀ» µ¿½Ã¿¡ ¶Ç´Â °³º°ÀûÀ¸·Î NGS Library Á¦ÀÛ °¡´É
  • No multiplex PCR required -1ȸ ¹ÝÀÀ´ç ´ÜÀÏ ÇÁ¶óÀ̸ӷΠ°¢°¢ÀÇ TCR SubunitÀ» ÁõÆø
  • Illumina¢ç-ready sequencing libraries - ligation-independent ¹æ½ÄÀ¸·Î illumina¢ç adaptor ¹× index sequence ºÎÂø °¡´É (Incorporate Illumina¢ç adapter and index sequences in a ligation-independent manner, and multiplex up to 96 libraries in a single flow-cell lane)
Á¦Ç°¼³¸í
SMARTer¢ç Mouse TCR a/b Profiling KitÀº T-cell receptor (TCR) repertoireÀÇ NGS ºÐ¼®À» À§ÇÑ Illumina platform NGS Library Á¦ÀÛ Å°Æ®ÀÌ´Ù. 5¡¯-RACE ±â¼úÀ» Á¢¸ñÇÏ¿© TCR Àü»çüÀÇ V(D)J °¡º¯¿µ¿ª (variable region)À» ºÐ¼®Çϸç, mouse spleen, thymus, or PBMCs·ÎºÎÅÍ ¾òÀº 10ng-500ngÀÇ total RNA ¶Ç´Â 1,000°³-10,000°³ÀÇ purified T-cellÀ» Àû¿ë °¡´ÉÇÏ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé TCR-¥á, TCR-¥â chainÀ» µ¿½Ã¿¡ ¶Ç´Â °¢ chain¿¡ ´ëÇÑ ¶óÀ̺귯¸®¸¦ °³º°ÀûÀ¸·Î Á¦ÀÛÇÏ¿© ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
Multiplexing PCRÀ» ÀÌ¿ëÇÏ´Â ±âÁ¸ÀÇ TCR profiling ¹æ¹ý°ú ´Þ¸®, library amplificationÀÇ °¢ ¹ÝÀÀÀº single primer pair¸¦ ÀÌ¿ëÇϹǷΠprimer-dimer »ý¼º °¡´É¼ºÀ» ³·Ãâ ¼ö ÀÖ´Ù. Multiplex PCR ¹æ½ÄÀº ¼­¿­Æ¯ÀÌÀû ÆíÇ⼺À» ³ªÅ¸³¾ °¡´É¼ºÀÌ ÀÖÀ¸³ª RACE ¹æ½ÄÀ» ÀÌ¿ëÇÒ °æ¿ì ÀÌ·¯ÇÑ ÆíÇ⼺ÀÇ ¹®Á¦¸¦ ´ú ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº LNA ±â¼úÀÌ °áÇÕµÈ SMART (Switching Mechanism at 5¡¯ End of RNA Template)¹ýÀ» ÀÌ¿ëÇϹǷΠ¹Î°¨µµ°¡ ¸Å¿ì ³ô°í ÆíÇ⼺ÀÌ ÀûÀº TCR mRNA sequence ºÐ¼®À» ÇÒ ¼ö ÀÖ´Ù.



±×¸² 1. Library preparation workflow and PCR strategy for TCR profiling using the SMARTer approach.


±×¸² 2. Assessment of TCR diversity in mouse spleen samples with the SMARTer Mouse TCR a/b Profiling Kit. Panel A. To compare TCR diversity between the spleen's general T-cell population and purified CD4+ T cells, total splenocytes were isolated from spleen and divided into two groups. The first group was left untouched, and the second group was subject to CD4+ T-cell purification (see Methods section for details). Total RNA was extracted from the total splenocyte group and the group enriched for CD4+ T cells, and each RNA sample was used for library preparation. Panel B. Proportion of on-target reads mapping to TCR-¥á or TCR-¥â CDR3 sequences. Panel C. Rarefaction curves showing the relationship between read depth and the number of unique clonotypes identified for TCR-¥á (left) and TCR-¥â (right). When a curve starts to flatten off, the number of unique clonotypes identified is reaching saturation. Dashed lines show the theoretical number of clonotypes as the number of reads increases.


±×¸² 3. Assessment of TCR-¥á clonotype diversity in various tissues of transgenic mice of different ages with the SMARTer Mouse TCR a/b Profiling Kit. Panel A. The percentage of reads mapping to TCR-¥á CDR3 sequences identified in the thymus, spleen, peripheral lymph nodes (PLN), mesenteric lymph nodes (MLN), and large intestine (LI) is consistent across the 16-week-old, 34-week-old, and 38-week-old mice. Panel B. The immune tissues showed the highest TCR-¥á clonotypic diversity, while the large intestine showed the lowest diversity. This indicates that the large intestine has the lowest T-cell infiltrate. Panel C. Abundance of the TRAV3-2-TRAJ58 clonotype, expressed as a percentage of all reads mapping to any TCR-¥á or TCR-¥â clonotype, across tissue types and ages. Panel D. Abundance of the TRAV16N-TRAJ56 clonotype, expressed as a percentage of all reads mapping to any TCR-¥á or TCR-¥â clonotype, across tissue types in the 38-week-old mouse.
Applications
Mouse TCR repertoire analysis (TCR-alpha and TCR-beta subunits)
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
SMARTer Mouse TCR a/b Profiling Kit Components
TCR a/b Mouse Indexing Primer Set HT for Illumina - 12

Keyword : TCR,T cell receptor,repertoire,variable region,Immune cell,Immune-seq,Immunology,,SMART,smart-seq,NGS,Next generation, sequencing,cancer,Cancer research,¾Ï¿¬±¸,vaccine,¹é½Å,¹é½Å¿¬±¸

Immune-Seq
>
RACE±â¼úÀ» È°¿ëÇÑ °í°¨µµ TCR repertoire ºÐ¼®
[New]UMI Àû¿ë Human TCR profiling(v3)
>
RACE±â¼úÀ» È°¿ëÇÑ °í°¨µµ BCR repertoire prof..
[New]UMI Àû¿ë Human BCR profiling(v2)
>
Mouse B-cell receptorÀÇ °í°¨µµ BCR repertoir..
[New]UMI Àû¿ë Mouse BCR profiling(v2)
>
TCR repertoire¸¦ Ź¿ùÇÑ °¨µµ·Î ºÐ¼®
UMI Àû¿ë Human TCR profiling(v2)
>
Human BCR V(D)J ¿µ¿ª ¼­¿­ ºÐ¼® (BCR profilin..
Human BCR IgG IgM H/K/L Profiling Kit(v1)
>
Mouse TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ NGS ¼­¿­ ºÐ..
Mouse TCR a/b Profiling Kit(v1)
>
Mouse B-cell receptorÀÇ Heavy chain, Light c..
Mouse BCR IgG H/K/L Profiling Kit(v1)