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Home
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ÀüÁ¦Ç°º¸±â
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NGS °ü·Ã
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Immune-Seq
> Mouse TCR a/b Profiling Kit
Mouse TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ NGS ¼¿ ºÐ¼®
Mouse TCR a/b Profiling Kit
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Clontech
634402
SMARTer¢ç Mouse TCR a/b Profiling Kit
12ȸ
1,528,800¿ø
1,911,000¿ø
05.02 ~ 06.30
Clontech
634403
SMARTer¢ç Mouse TCR a/b Profiling Kit
48ȸ
4,936,000¿ø
6,170,000¿ø
05.02 ~ 06.30
Clontech
634404
SMARTer¢ç Mouse TCR a/b Profiling Kit
96ȸ
8,045,600¿ø
10,057,000¿ø
05.02 ~ 06.30
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
[FAQ] RNA-seq
Cancer ¿¬±¸ °¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
SMARTer RNA-seqÀÇ HOT ..
mRNA-Seq (Single cell)
[Cancer Research] Canc..
Single cell-seq °¡À̵å
Single cell SMART-seq..
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
Single cell RNA-seq¿¡ ..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
[Cancer Research] Canc..
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Cancer Research] Immu..
[Version 2] Human TCR ..
Human TCR a/b Profilin..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
SMART-Seq Human BCR (w..
SMART-Seq Human TCR (w..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
Single cell-seq °¡À̵å
[FAQ] DNA-Seq
[Àû¿ë] Overview_ThruPL..
[Cancer Research] Canc..
DNA-seqÀÇ ´Ù¾çÇÑ ÃֽŠ..
ChIP-Seq / Meth-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[³í¹®] ThruPLEX¢ç for ..
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
[Cancer Research] Canc..
Single cell-seq °¡À̵å
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
[Cancer Research] HLA ..
[³í¹®] PicoPLEX¢ç ±â¼ú..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
[FAQ] Library index
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
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SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Citation
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) SMARTer¢ç Str..
(Legacy) PicoPLEX¢ç DN..
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Complete V(D)J sequence information -
TCR mRNAÀÇ °¡º¯¿µ¿ª(variable regions)ÀÇ Àüü ¼¿(Full length) ºÐ¼®
TCR-alpha, TCR-beta chain
À» µ¿½Ã¿¡ ¶Ç´Â °³º°ÀûÀ¸·Î NGS Library Á¦ÀÛ °¡´É
No multiplex PCR required -
1ȸ ¹ÝÀÀ´ç ´ÜÀÏ ÇÁ¶óÀ̸ӷΠ°¢°¢ÀÇ TCR SubunitÀ» ÁõÆø
Illumina¢ç-ready sequencing libraries -
ligation-independent ¹æ½ÄÀ¸·Î illumina¢ç adaptor ¹× index sequence ºÎÂø °¡´É (Incorporate Illumina¢ç adapter and index sequences in a ligation-independent manner, and multiplex up to 96 libraries in a single flow-cell lane)
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SMARTer¢ç Mouse TCR a/b Profiling KitÀº T-cell receptor (TCR) repertoireÀÇ NGS ºÐ¼®À» À§ÇÑ Illumina platform NGS Library Á¦ÀÛ Å°Æ®ÀÌ´Ù. 5¡¯-RACE ±â¼úÀ» Á¢¸ñÇÏ¿© TCR Àü»çüÀÇ V(D)J °¡º¯¿µ¿ª (variable region)À» ºÐ¼®Çϸç, mouse spleen, thymus, or PBMCs·ÎºÎÅÍ ¾òÀº 10ng-500ngÀÇ total RNA ¶Ç´Â 1,000°³-10,000°³ÀÇ purified T-cellÀ» Àû¿ë °¡´ÉÇÏ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé TCR-¥á, TCR-¥â chainÀ» µ¿½Ã¿¡ ¶Ç´Â °¢ chain¿¡ ´ëÇÑ ¶óÀ̺귯¸®¸¦ °³º°ÀûÀ¸·Î Á¦ÀÛÇÏ¿© ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
Multiplexing PCRÀ» ÀÌ¿ëÇÏ´Â ±âÁ¸ÀÇ TCR profiling ¹æ¹ý°ú ´Þ¸®, library amplificationÀÇ °¢ ¹ÝÀÀÀº single primer pair¸¦ ÀÌ¿ëÇϹǷΠprimer-dimer »ý¼º °¡´É¼ºÀ» ³·Ãâ ¼ö ÀÖ´Ù. Multiplex PCR ¹æ½ÄÀº ¼¿Æ¯ÀÌÀû ÆíÇ⼺À» ³ªÅ¸³¾ °¡´É¼ºÀÌ ÀÖÀ¸³ª RACE ¹æ½ÄÀ» ÀÌ¿ëÇÒ °æ¿ì ÀÌ·¯ÇÑ ÆíÇ⼺ÀÇ ¹®Á¦¸¦ ´ú ¼ö ÀÖ´Ù.
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echanism
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emplate)¹ýÀ» ÀÌ¿ëÇϹǷΠ¹Î°¨µµ°¡ ¸Å¿ì ³ô°í ÆíÇ⼺ÀÌ ÀûÀº TCR mRNA sequence ºÐ¼®À» ÇÒ ¼ö ÀÖ´Ù.
±×¸² 1. Library preparation workflow and PCR strategy for TCR profiling using the SMARTer approach.
±×¸² 2. Assessment of TCR diversity in mouse spleen samples with the SMARTer Mouse TCR a/b Profiling Kit.
Panel A. To compare TCR diversity between the spleen's general T-cell population and purified CD4+ T cells, total splenocytes were isolated from spleen and divided into two groups. The first group was left untouched, and the second group was subject to CD4+ T-cell purification (see Methods section for details). Total RNA was extracted from the total splenocyte group and the group enriched for CD4+ T cells, and each RNA sample was used for library preparation. Panel B. Proportion of on-target reads mapping to TCR-¥á or TCR-¥â CDR3 sequences. Panel C. Rarefaction curves showing the relationship between read depth and the number of unique clonotypes identified for TCR-¥á (left) and TCR-¥â (right). When a curve starts to flatten off, the number of unique clonotypes identified is reaching saturation. Dashed lines show the theoretical number of clonotypes as the number of reads increases.
±×¸² 3. Assessment of TCR-¥á clonotype diversity in various tissues of transgenic mice of different ages with the SMARTer Mouse TCR a/b Profiling Kit.
Panel A. The percentage of reads mapping to TCR-¥á CDR3 sequences identified in the thymus, spleen, peripheral lymph nodes (PLN), mesenteric lymph nodes (MLN), and large intestine (LI) is consistent across the 16-week-old, 34-week-old, and 38-week-old mice. Panel B. The immune tissues showed the highest TCR-¥á clonotypic diversity, while the large intestine showed the lowest diversity. This indicates that the large intestine has the lowest T-cell infiltrate. Panel C. Abundance of the TRAV3-2-TRAJ58 clonotype, expressed as a percentage of all reads mapping to any TCR-¥á or TCR-¥â clonotype, across tissue types and ages. Panel D. Abundance of the TRAV16N-TRAJ56 clonotype, expressed as a percentage of all reads mapping to any TCR-¥á or TCR-¥â clonotype, across tissue types in the 38-week-old mouse.
Applications
Mouse TCR repertoire analysis (TCR-alpha and TCR-beta subunits)
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
SMARTer Mouse TCR a/b Profiling Kit Components
TCR a/b Mouse Indexing Primer Set HT for Illumina - 12
Keyword : TCR,T cell receptor,repertoire,variable region,Immune cell,Immune-seq,Immunology,,SMART,smart-seq,NGS,Next generation, sequencing,cancer,Cancer research,¾Ï¿¬±¸,vaccine,¹é½Å,¹é½Å¿¬±¸
Immune-Seq
[¼±Åð¡À̵å] Immune-seq
[Cancer Research] Immunotherapy
TCR repertoire¸¦ Ź¿ùÇÑ °¨µµ·Î ºÐ¼®
[Version 2] Human TCR a/b Profiling Kit v2
TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ Full-length seque..
Human TCR a/b Profiling Kit
Human BCR V(D)J ¿µ¿ª ¼¿ ºÐ¼® (BCR profilin..
Human BCR IgG IgM H/K/L Profiling Kit
Mouse TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ NGS ¼¿ ºÐ..
Mouse TCR a/b Profiling Kit
Mouse B-cell receptorÀÇ Heavy chain, Light c..
Mouse BCR IgG H/K/L Profiling Kit
RACE±â¼úÀ» Ȱ¿ëÇÑ °í°¨µµ BCR repertoire prof..
SMART-Seq Human BCR (with UMIs)
RACE±â¼úÀ» Ȱ¿ëÇÑ °í°¨µµ TCR repertoire ºÐ¼®
SMART-Seq Human TCR (with UMIs)