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NGS °ü·Ã
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NGS Library Á¤·®
> Library Quantification Kit (for Illumina)
Illumina¿ë NGS Library Á¤·® Å°Æ®
Library Quantification Kit (for Illumina)
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Clontech
638324
Library Quantification Kit
500 ȸ
(Package)
1,164,000¿ø
Clontech
638325
DNA Standards for Library Quantification
50 ȸ
448,000¿ø
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PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
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[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Single cell mRNA-Seq K..
Ultra low input mRNA-S..
High throughput, Autom..
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Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
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Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
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Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
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Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
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Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç WG..
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Highly sensitive quantification of Illumina sequencing libraries
- the qPCR-based assay can measure even low concentration libraries
Allows specific quantification of only DNA molecules bound to adapters
Reduces variability in cluster density to improve sequencing results
- use prior to sequencing to ensure optimal loading concentration
Complete kit
- includes qPCR primers and reagents, and DNA standards for absolute quantification using a standard curve
Àû¿ë
Illumina NGS library quantification by qPCR
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High-qualityÀÇ Illumina next-generation sequencing µ¥ÀÌÅ͸¦ ¾ò±â À§ÇÏ¿©, ÀûÀýÇÑ ¾çÀÇ library DNA¸¦ ÀÌ¿ëÇÏ´Â °ÍÀÌ ÇʼöÀûÀÌ´Ù. Library DNAÀÇ ¾çÀÌ ºÎÁ·Çϸé cluster ¹Ðµµ (cluster density)°¡ ³·¾ÆÁ® ½ÃÄö½Ì ¼öÀ²(sequencing yield)ÀÌ ³·¾ÆÁø´Ù. ¹Ý¸é, Library DNAÀÇ ¾çÀÌ ³Ê¹« ¸¹À¸¸é Cluster ¹Ðµµ°¡ ³ô¾ÆÁ® High-qualityÀÇ µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ¾ø´Ù. Àü±â¿µµ¿(Electrophoresis)À̳ª ºÐ±¤ ±¤µµ ÃøÁ¤(spectrophotometry)°ú °°Àº ±âÁ¸ÀÇ NGS Library Á¤·® ¹æ¹ýÀº sensitivity°¡ ³·°í, adaptor-bound DNA¿¡ ´ëÇÏ¿© ºñƯÀÌÀû ÀϻӸ¸ ¾Æ´Ï¶ó, ¸¹Àº ¾çÀÇ Library DNA°¡ ÇÊ¿äÇÏ´Ù.
Library Quantification KitÀº °í°¨µµÀÇ qPCR ±â¹ÝÀÇ Á¤·®¹ýÀ» »ç¿ëÇϱ⠶§¹®¿¡ IlluminaÀÇ NGS ¹ÝÀÀ¿¡ »ç¿ëµÇ´Â adaptor sequence¸¦ ƯÀÌÀûÀ¸·Î °ËÃâÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, »ùÇà ³»¿¡ adaptor°¡ ºÎ°¡µÇÁö ¾ÊÀº DNAºÐÀÚ°¡ ÀÖ´õ¶óµµ sequencing libraryÀÇ ³óµµ¸¦ ÃøÁ¤ °¡´ÉÇϸç, library Á¦ÀÛ °úÁ¤¿¡¼ adaptor ligationÀ» È®ÀÎÇÏ´Â µ¥ »ç¿ëÇÒ ¼öµµ ÀÖ´Ù.
º» Á¦Ç°¿¡´Â standard curve ÀÛ¼ºÀ» À§ÇÑ DNA Standard°¡ Æ÷ÇԵǾîÀÖ¾î, library DNAÀÇ Àý´ëÁ¤·®ÀÌ °¡´ÉÇϸç, DNA standards for Library Quantification(Code 638325)´Â º°µµ·Î ±¸¸Å °¡´ÉÇÏ´Ù.
±×¸² 1. A typical RNA-seq workflow.
Generating sufficient amounts of library DNA and loading an appropriate amount on the flow cell is essential for obtaining high-quality NGS data. The Library Quantification Kit, a qPCR-based quantification method that targets Illumina¡¯s adapter sequences, can be used to measure library concentration and ensure optimal loading concentration
±×¸² 2. Lot-to-lot variation for DNA Standards.
Quantification of three lots of pre-diluted DNA Standards (0.01, 0.1, 1, and 10 pM) was performed by real-time PCR using Terra qPCR Direct SYBR Premix following the protocol in the Library Quantification Kit user manual. All reactions were performed in triplicate, and the resulting Ct (SDM) values for the triplicates were averaged. The variation between lots was <5%.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ »çÇ×Àº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
Library Quantification Kit (Code 638324)
Terra qPCR Direct SYBR Premix (Code 638319)
Primer Mix Kit
DNA Standards for Library Quantification (Code 638325)
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Terra qPCR Direct SYBR Premix: -70¡É (À¶ÇØ ÈÄ, 4¡É¿¡¼ 6°³¿ù º¸Á¸)
±âŸ: -20¡É
Keyword : Library Á¤·®,Illumina ngs,ÀÏ·ç¹Ì³ª Á¤·®,illumina,illumina qpcr
NGS Library Á¤·®
Illumina¿ë NGS Library Á¤·® Å°Æ®
Library Quantification Kit (for Illumina)