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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS °ü·Ã Àåºñ > ICELL8¢ç cx Single-Cell System
SmartChip ±â¼úÀ» ÀÌ¿ëÇÑ Single-cell ºÐ¼® ½Ã½ºÅÛ

ICELL8¢ç cx Single-Cell System

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Takara
640188
ICELL8¢ç cx Single-Cell System
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
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°¡°Ý¹®ÀÇ   x105932, x107503, x335982


ICELL8¢ç cx Single-Cell SystemÀº 5,184°³ÀÇ nano wellÀÌ Å¾ÀçµÈ SmartChip¿¡ single cell ºÐ¸®¸¦ À§ÇÑ dispense¿Í smartchip¿¡ ºÐ¸®µÈ single cellÀ» ±¸ºÐÇϱâ À§ÇÑ imaging±â´ÉÀÌ °áÇÕµÈ single cell NGS¿¬±¸¸¦ À§ÇÑ ÀÚµ¿È­ platformÀÔ´Ï´Ù. ICELL8 cx ½Ã½ºÅÛÀº ±ÕÀÏÇÏÁö ¾ÊÀº Å©±âÀÇ ¼¼Æ÷¸¦ single cellÀ» ºÐ¸®Çϱâ À§ÇØ large bore nozzleÀÇ dispenser¸¦ »ç¿ëÇÕ´Ï´Ù. Imaging±â´ÉÀ» ³»ÀåÇÏ¿© SmartChip¿¡ ºÐ¸®µÈ ¼öõ °³ÀÇ cellÁß¿¡¼­ ´ÜÀÏ ¼¼Æ÷ ¿©ºÎ¸¦ ±¸º°ÇÒ ¼ö ÀÖ½À´Ï´Ù. ¶ÇÇÑ, cell¿¡ Çü±¤¿°»öÀ» ÇÑ ÈÄ, Çü±¤ °ËÃâÀ» ÅëÇØ ºÐ¸®µÈ Single cellÀÇ live / dead »óÅ¿¡ ´ëÇÑ È®Àεµ °¡´ÉÇÕ´Ï´Ù.


Àüü ½Ã½ºÅÛ¿¡´Â imagingÀ» À§ÇÑ ÃÔ¿µ½Ã½ºÅÛÀÌ ³»ÀåµÇ¾î ÀÖÀ¸¸ç, ÃÔ¿µµÈ image¸¦ ºÐ¼®ÇÏ°í ¼±ÅÃµÈ well¸¸ ÁøÇàÇÒ ¼ö ÀÖ´Â ICELL8 cx CellSelect Software°¡ Æ÷ÇԵǾî ÀÖ¾î multiple-cell ¶Ç´Â empty well µîÀÇ NGS ºÐ¼®½Ã °É¸²µ¹ÀÌ µÉ ¼ö ÀÖ´Â ³ëÀÌÁ »çÀü¿¡ Á¦°ÅÇÒ ¼ö ÀÖ½À´Ï´Ù. ÀÌ °°Àº Single Cell NGS¿¬±¸¸¦ À§ÇÑ High-throughput open platformÀº ¿©·¯ SMARTer ±â¼úÀ» ÀÌ¿ëÇÑ NGS application°ú ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó, ¿¬±¸ÀÚ°¡ ±âÁ¸ application¿¡ ÇÑÁ¤µÇÁö ¾Ê°í µ¶ÀÚÀûÀÎ applicationÀ» °³¹ßÇÒ ¼ö ÀÖ´Â ÁøÁ¤ÇÑ OPEN PlatformÀÔ´Ï´Ù.
ICELL8 cx ½Ã½ºÅÛ overview µ¿¿µ»ó
ICELL8 cx systemÀ» ÀÌ¿ëÇÑ scRNA-Seq Work-flow


1) Cell isolation

¿¬±¸ÀÚ°¡ ÁغñÇÑ Cell suspensionÀ» ½Ã½ºÅÛ¿¡ ÀåÂøÇÏ°í ICELL8 chip¿¡ ºÐÁÖÇÏ´Â °úÁ¤. ºÐÁÖ ½Ã, Dispenser°¡ 8°³ ä³ÎÀÇ nozzleÀÌ Àֱ⠶§¹®¿¡, ÃÖ´ë 8°³ »ùÇÃÀ» µ¿½Ã¿¡ ó¸®ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, »ç¿ëÀÚÀÇ »ùÇà »Ó¸¸ ¾Æ´Ï¶ó, NGS ¿¬±¸¿Í ºÐ¼®½Ã ¹Ýµå½Ã ÇÊ¿äÇÑ Positive Control, Negative ControlÀ» »ç¿ëÇÒ ¼ö ÀÖ¾î, ¿¬±¸°á°úÀÇ ½Å·Úµµ¸¦ Çâ»ó ½Ãų ¼ö ÀÖ´Ù. ºÒ°ú 15ºÐ ¾È¿¡ 5,184°³ well ¼¼Æ÷¸¦ ºÐÁÖÇÏ´Â ÀÛ¾÷°ú positive/negative controlÀÇ ºÐÁÖ°¡ ¿Ï·áµË´Ï´Ù.

2) Imaging

CellÀ» ºÐÁÖÇÑ chipÀ» ½Ã½ºÅÛ¿¡ ³»ÀåµÈ imagingÀåÄ¡¸¦ ÀÌ¿ëÇÏ¿© ÃÔ¿µ ÈÄ, ÃÔ¿µµÈ image¸¦ CellSelect ¼ÒÇÁÆ®¿þ¾î°¡ ÀÚµ¿À¸·Î ºÐ¼®, °¢ well¿¡ ºÐÁÖµÈ cellÀÇ °³¼ö¿Í live/dead »óŸ¦ ÀÚµ¿À¸·Î ÆÇÁ¤ÇÏ¿© »ì¾ÆÀÖ´Â single cell¸¸ NGS library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï RT reagent ÷°¡ µîÀÇ downstreamÀ¸·Î ÁøÇàÇÕ´Ï´Ù. ÀÌ·¯ÇÑ Imaging¿¡ ¼Ò¿äµÇ´Â ½Ã°£Àº ºÒ°ú ¾à 15ºÐÀÔ´Ï´Ù.

3) Cell Select

ÃÔ¿µµÈ chipÀÇ À̹ÌÁö¸¦ CellSelect SW°¡ ÀÚµ¿À¸·Î ºÐ¼®, »ó±â¿Í °°ÀÌ °¢ well¿¡ ºÐ¸®µÈ ¼¼Æ÷ÀÇ °³¼ö¿Í °¢ ¼¼Æ÷ÀÇ »ý»ç¿©ºÎ¸¦ ÀÚµ¿À¸·Î È®ÀÎÇÕ´Ï´Ù. »ì¾ÆÀÖ´Â ´ÜÀϼ¼Æ÷°¡ ºÐ¸®µÇ¾î ÀÖ´Â well¸¸ ¼±ÅÃÇÏ¿© NGS¸¦ ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.
  • CellSelect SW µ¿¿µ»ó

4) RT reagent Dispense
ºÐ¼®ÇÏ°íÀÚ ÇÏ´Â ´ÜÀϼ¼Æ÷°¡ Æ÷ÇÔµÈ well¸¸ ÁöÁ¤ÇÏ¿© RT ½Ã¾àÀ» ºÐÁÖÇÒ ¼ö ÀÖ½À´Ï´Ù. ±×·¯¹Ç·Î, ´ÜÀϼ¼Æ÷°¡ ¾Æ´Ñ Multiple cell, empty cellÀÌ Æ÷ÇÔµÈ well¿¡ RT ½Ã¾à ºÐÁÖ°¡ ÀÌ·ïÁöÁö ¾Ê¾Æ, cost¸¦ Àý¾àÇÒ ÀÖÀ¸¸ç ÇâÈÄ ºÐ¼® ½Ã ´ÜÀϼ¼Æ÷°¡ ¾Æ´Ñ ¼¼Æ÷·ÎºÎÅÍÀÇ °á°ú·Î ÀÎÇÑ ºÐ¼®ÀÇ ¿À·ù¸¦ »çÀü¿¡ Â÷´ÜÇÒ ¼ö ÀÖ½À´Ï´Ù.

5) cDNA Synthesis, collect
Àü¿ë chip adaptor°¡ ÀåÂøµÈ chip cycler¸¦ ÀÌ¿ë, RT ½Ã¾àÀÌ Ã·°¡µÈ sinlge cellÀ» Reverse transcription ¹ÝÀÀÀ» ÅëÇØ cDNA¸¦ ÇÕ¼ºÇϸ鼭 °¢°¢ÀÇ À¯ÀüÀÚ¿¡ UMI¿Í WBC µî sequencingÈÄ °¢°¢ÀÇ single cell À¯ÀüÀÚÀÇ ±¸ºÐÀÌ °¡´ÉÇÑ indexing±îÁö ¿Ï·áµË´Ï´Ù.

Chip Cycler¿¡¼­ RT¸¦ ¸¶Ä£ chipÀº Àü¿ë collector¸¦ ÀÌ¿ë, ÇÑ tube¿¡ poolingÇÏ¿© library Á¦ÀÛÀ» ¸¶Ä£ ÈÄ NGS sequencer¿¡¼­ sequencingÀ» ¼öÇàÇÑ´Ù.
»ç¿ë °¡´ÉÇÑ Application
  • 3¡¯ Differential Expression (DE)
  • Single Cell T-Cell Receptor profiling
  • Full-Length Transcriptome Analysis
  • ATAC-Seq
  • Cut&Tag Analysis






Keyword : Single cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,automation,single cell isolation,imageNGS,Next Generation Sequencing,SMART,SMARTer,Rubicon,·çºñÄÜ