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Whole genome amplification (WGA) from single cells

PicoPLEX¢ç Single Cell WGA Kit

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Clontech
R300671
PicoPLEX¢ç Single Cell WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
°¡°Ý¹®ÀÇ   Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x102538
Clontech
R300672
PicoPLEX¢ç Single Cell WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
°¡°Ý¹®ÀÇ   Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R300673
PicoPLEX¢ç Single Cell WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
480ȸ
°¡°Ý¹®ÀÇ   Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

* Àá±ñ, ù ±¸¸ÅÀΰ¡¿ä? ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (Tel. 02-2081-2510)·Î ¿¬¶ôÁÖ¼¼¿ä!

* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX¢ç WGA Kit (Code R30050)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.


  • Reproducible results from single cells (or <15 pg of DNA)
  • Superior reproducibility of allele representation
  • Easy to use and automate
  • Unambiguous results at all resolutions (e.g., microarray and PCR)
Á¦Ç°¼³¸í
PicoPLEX¢ç Single Cell WGA Kit´Â ´ÜÀϼ¼Æ÷ (single cell)·ÎºÎÅÍ ³ôÀº ÀçÇö¼º°ú Á¤È®µµ·Î Genomic DNA¸¦ ÁõÆø °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù. º» Á¦Ç°ÀÇ ±âº»ÀÌ µÇ´Â °íÀ¯ÀÇ ±â¼ú(ƯÇã ±â¼ú), PicoPLEX¢ç ±â¼úÀº single copy genomic DNAÀÇ ÁõÆø¿¡ ÃÖÀûÈ­µÇ¾î ÀÖÀ¸¸ç ÇϳªÀÇ tube¿¡¼­ ¹ÝÀÀÇÏ´Â single tube reactionÀ¸·Î »ç¿ëÇÏ±â Æí¸®Çϰí, ¿¡·¯ ¹× ¹é±×¶ó¿îµå¸¦ ÃÖ¼ÒÈ­ÇÏ¿© Çâ»óµÈ °á°ú¸¦ µµÃâÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº single cell (1 - 10 cells)»Ó ¾Æ´Ï¶ó, ÃßÃâÇÑ ±Ø¼Ò·®(6 - 50 pg)ÀÇ gDNA·ÎºÎÅÍ Whole Genome Amplification (WGA)¸¦ ÁøÇàÇÒ ¼ö ÀÖÀ¸¸ç, ÁõÆøÇÑ DNA´Â PCRÀº ¹°·Ð array CGH analysis¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
PicoPLEX¢ç WGA KitÀº in vitro fertilization(IVF, ü¿Ü¼öÁ¤)½Ã pre-implantation genetic screening and diagnostics (PGS/PGD)¿Í °°Àº °Ë»ç ½Ã À¯¿ëÇÏ°Ô »ç¿ëµÇ°í ÀÖ´Ù.


±×¸² 1. PicoPLEX¢ç WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (PicoPLEX¢ç ±â¼ú °³¿ä º¸±â)


±×¸² 2. Reproducible amplification
Flow-sorted cancer cells were amplified with PicoPLEX single cell WGA technology. Each of the cells (blue lines) amplified at the same rate and resulted in a similar, predictable yield. A pooled sample of five cells (red line) represents a positive control. Samples containing 0 cells (green lines, no template control) show very low background.


±×¸² 3. Reproducible CNVs
Single-blastomere biopsies were amplified using PicoPLEX single cell WGA technology, labeled and hybridized to BlueGnome¡¯s 24 Sure arrays at Genesis Genetics Institute. Note the clear indiciation of CNVs. In 2011 ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX technology.


±×¸² 4. Greater reliability
Locus-specific qPCR was used to quantify 48 loci in independent single-cell libraries. Data shown compares results of DNA from two individual samples amplified with (left to right) PicoPLEX single cell WGA technology, GenomePLEX, and GehomiPhi using 10pg of DNA. More than 90% of the product from the PicoPLEX single cell WGA technology produced identifiable, highly reproducible human sequences.
Applications
  • Copy Number Variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
  • Target enrichment or amplicon enrichment for mutation detection
  • SNP Genotyping
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- Cell Extraction Buffer
- Extraction Enzyme Dilution Buffer
- Cell Extraction Enzyme
- Pre-Amp W Buffer
- Pre-Amp W Enzyme
- Amplification Buffer
- Amplification Enzyme
- Nuclease-Free Water

Keyword : Sngle cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation

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