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Whole genome amplification (WGA) from single cells

PicoPLEX® Single Cell WGA Kit

제조사	제품코드	제품명	용량	가격 (부가세별도)
Clontech	R300671	PicoPLEX® Single Cell WGA Kit	24회	가격문의
Clontech	R300672	PicoPLEX® Single Cell WGA Kit	96회	가격문의
Clontech	R300673	PicoPLEX® Single Cell WGA Kit	480회	가격문의

Product index

PGT-A
- Embgenix™ PGT-A Kit (..
SMARTer RNA-Seq 개요
mRNA-Seq (Single cell)
Stranded RNA-seq
Small RNA-Seq
- [Cancer Research] Canc..
- Small RNA-Seq Kit
Immune-Seq
기타 RNA-Seq
- Full length cDNA 합성
- Target RNA Capture for..
DNA-seq 개요
ChIP-Seq / Meth-Seq
Low Input DNA-Seq
High input DNA-seq
- ThruPLEX® DNA-Seq HV
- ThruPLEX® Tag-Seq HV
Single cell DNA-Seq
Whole Genome Amplification..
Index Kit
NGS Library 정량
- Library Quantification..
NGS Sample preparation
NGS 분석용 PCR 효소
NGS 관련 장비
[Legacy] NGS Products

* 본 제품은 기존 PicoPLEX^® WGA Kit (Code R30050)의 업그레이드 버전 제품입니다.

Reproducible results from single cells (or <15 pg of DNA)
Superior reproducibility of allele representation
Easy to use and automate
Unambiguous results at all resolutions (e.g., microarray and PCR)

제품설명

PicoPLEX^® Single Cell WGA Kit는 단일세포 (single cell)로부터 높은 재현성과 정확도로 Genomic DNA를 증폭 가능한 제품이다. 본 제품의 기본이 되는 고유의 기술(특허 기술), PicoPLEX^® 기술은 single copy genomic DNA의 증폭에 최적화되어 있으며 하나의 tube에서 반응하는 single tube reaction으로 사용하기 편리하고, 에러 및 백그라운드를 최소화하여 향상된 결과를 도출할 수 있다. 본 제품은 single cell (1 - 10 cells)뿐 아니라, 추출한 극소량(6 - 50 pg)의 gDNA로부터 Whole Genome Amplification (WGA)를 진행할 수 있으며, 증폭한 DNA는 PCR은 물론 array CGH analysis에도 적용할 수 있다.
PicoPLEX^® WGA Kit은 in vitro fertilization(IVF, 체외수정)시 pre-implantation genetic screening and diagnostics (PGS/PGD)와 같은 검사 시 유용하게 사용되고 있다.

그림 1. PicoPLEX^® WGA기술의 원리
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (PicoPLEX^® 기술 개요 보기)

그림 2. Reproducible amplification
Flow-sorted cancer cells were amplified with PicoPLEX single cell WGA technology. Each of the cells (blue lines) amplified at the same rate and resulted in a similar, predictable yield. A pooled sample of five cells (red line) represents a positive control. Samples containing 0 cells (green lines, no template control) show very low background.

그림 3. Reproducible CNVs
Single-blastomere biopsies were amplified using PicoPLEX single cell WGA technology, labeled and hybridized to BlueGnome’s 24 Sure arrays at Genesis Genetics Institute. Note the clear indiciation of CNVs. In 2011 ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX technology.

그림 4. Greater reliability
Locus-specific qPCR was used to quantify 48 loci in independent single-cell libraries. Data shown compares results of DNA from two individual samples amplified with (left to right) PicoPLEX single cell WGA technology, GenomePLEX, and GehomiPhi using 10pg of DNA. More than 90% of the product from the PicoPLEX single cell WGA technology produced identifiable, highly reproducible human sequences.

Applications

Copy Number Variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
Target enrichment or amplicon enrichment for mutation detection
SNP Genotyping

구성품 (자세한 내용은 CoA를 참조하세요)

- Cell Extraction Buffer
- Extraction Enzyme Dilution Buffer
- Cell Extraction Enzyme
- Pre-Amp W Buffer
- Pre-Amp W Enzyme
- Amplification Buffer
- Amplification Enzyme
- Nuclease-Free Water

Citation

PicoPLEX^® WGA Kit는 PicoPLEX^® Single Cell WGA Kit로 업데이트되었으며, 가장 최신버전은 PicoPLEX^® Single Cell WGA Kit v3입니다. Citation이 동일합니다.

Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis
American Journal of Human Genetics    |    2019 Dec 05    |    PubMed ID: 31785788    |    Read Article

Mutated clones driving leukemic transformation are already detectable at the single-cell level in CD34-positive cells in the chronic phase of primary myelofibrosis
NPJ Precision Oncology    |    2021 Feb 04    |    PubMed ID: 33542466    |    Read Article

Investigation of somatic CNVs in brains of synucleinopathy cases using targeted SNCA analysis and single cell sequencing
Acta Neuropathologica Communications    |    2019 Dec 23    |    PubMed ID: 31870437    |    Read Article

Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure
Human Reproduction    |    2019 Dec 01    |    PubMed ID: 31811307    |    Read Article

Binding of a C-type lectin’s coiled-coil domain to the Domeless receptor directly activates the JAK/STAT pathway in the shrimp immune response to bacterial infection
PLoS Pathogens    |    2017 Sep 20    |    PubMed ID: 28931061    |    Read Article

SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit
Horticulture Research    |    2021 Dec 01    |    PubMed ID: 34848692    |    Read Article

Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level, et al. Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level
Cancer Science    |    2021 Apr 02    |    PubMed ID: 33484069    |    Read Article

Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC-chip” for primary lung cancer, et al. Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC-chip” for primary lung cancer
Cancer Science    |    2022 Jan 28    |    PubMed ID: 34964211    |    Read Article

Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
Frontiers in Cell and Developmental Biolo…    |    2021 Dec 03    |    PubMed ID: 34926439    |    Read Article

Single-Circulating Tumor Cell Whole Genome Amplification to Unravel Cancer Heterogeneity and Actionable Biomarkers
International Journal of Molecular Scienc…    |    2022 Jul 29    |    PubMed ID: 35955517    |    Read Article

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices
International Journal of Molecular Scienc…    |    2022 Dec 01    |    PubMed ID: 36499466    |    Read Article

Genomic Analysis of Hematopoietic Stem Cell at the Single-Cell Level: Optimization of Cell Fixation and Whole Genome Amplification (WGA) Protocol
International Journal of Molecular Scienc…    |    2020 Oct 06    |    PubMed ID: 33036143    |    Read Article

Functional Analysis of the Active Site of a Metallo-β-Lactamase Proliferating in Japan
Antimicrobial Agents and Chemotherapy    |    1999 Jul 06    |    PubMed ID: 10952572    |    Read Article

A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples
Scientific Reports    |    2019 Aug 20    |    PubMed ID: 31431640    |    Read Article

Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter
Scientific Reports    |    2019 Dec 27    |    PubMed ID: 31882696    |    Read Article

Kinship analysis on single cells after whole genome amplification
Scientific Reports    |    2020 Sep 04    |    PubMed ID: 32887915    |    Read Article

First detection of TBE virus in ticks and sero-reactivity in goats in a non-endemic region in the southern part of Switzerland (Canton of Ticino).
Ticks and tick-borne diseases    |    2019 Aug 15    |    PubMed ID: 31047827    |    Read Article

Serological Investigation and Genetic Characteristics of Pseudorabies Virus in Hunan Province of China From 2016 to 2020
Frontiers in Veterinary Science    |    2021 Dec 16    |    Read Article

A common variant of CNTNAP2 is associated with sub-threshold autistic traits and intellectual disability
PLoS ONE    |    2021 Dec 13    |    PubMed ID: 34898614    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with Single Circulating Trophoblast (SCT) as one form of cell-based NIPT
PLoS ONE    |    2021 Apr 15    |    PubMed ID: 33857205    |    Read Article

Haplotyping by linked-read sequencing (HLRS) of the genetic disease carriers for preimplantation genetic testing without a proband or relatives
BMC Medical Genomics    |    2020 Aug 20    |    PubMed ID: 32819358    |    Read Article

A novel method for noninvasive diagnosis of monogenic diseases from circulating fetal cells.
Prenatal diagnosis    |    2021 Dec 10    |    PubMed ID: 32673403    |    Read Article

Cell-based non-invasive prenatal diagnosis in a pregnancy at risk of cystic fibrosis.
Prenatal diagnosis    |    2022 Jan 03    |    PubMed ID: 33150588    |    Read Article

Reliable detection of subchromosomal deletions and duplications using cell-based noninvasive prenatal testing
Prenatal Diagnosis    |    2018 Nov 19    |    PubMed ID: 30357877    |    Read Article

Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification
Malaria Journal    |    2020 May 27    |    PubMed ID: 32460780    |    Read Article

A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells
Micromachines    |    2019 Feb 17    |    PubMed ID: 30781548    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with single circulating trophoblast (SCT) prenatal diagnosis
medRxiv    |    2020 Jun 02    |    Read Article

Intercellular extrachromosomal DNA copy number heterogeneity drives cancer cell state diversity
bioRxiv    |    2023 Jan 21    |    Read Article

Artificial intelligence-driven morphology-based enrichment of malignant cells from body fluid
bioRxiv    |    2023 Jan 25    |    Read Article

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Keyword : Sngle cell,단일세포,싱글셀,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,태아산전검사,산전검사,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation

Whole Genome Amplification (WGA)

[기술개요] ThruPLEX & PicoPLEX

[Cancer Research] HLA typing

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