´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Whole genome amplification (WGA) from single cells

PicoPLEX¢ç Single Cell WGA Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
R300671
PicoPLEX¢ç Single Cell WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
1,144,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x102538
Clontech
R300672
PicoPLEX¢ç Single Cell WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
3,485,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R300673
PicoPLEX¢ç Single Cell WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
480ȸ (R300672 x 5)
15,683,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX¢ç WGA Kit (Code R30050)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.


  • Reproducible results from single cells (or <15 pg of DNA)
  • Superior reproducibility of allele representation
  • Easy to use and automate
  • Unambiguous results at all resolutions (e.g., microarray and PCR)
Á¦Ç°¼³¸í
PicoPLEX¢ç Single Cell WGA Kit´Â ´ÜÀϼ¼Æ÷ (single cell)·ÎºÎÅÍ ³ôÀº ÀçÇö¼º°ú Á¤È®µµ·Î Genomic DNA¸¦ ÁõÆø °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù. º» Á¦Ç°ÀÇ ±âº»ÀÌ µÇ´Â °íÀ¯ÀÇ ±â¼ú(ƯÇã ±â¼ú), PicoPLEX¢ç ±â¼úÀº single copy genomic DNAÀÇ ÁõÆø¿¡ ÃÖÀûÈ­µÇ¾î ÀÖÀ¸¸ç ÇϳªÀÇ tube¿¡¼­ ¹ÝÀÀÇÏ´Â single tube reactionÀ¸·Î »ç¿ëÇϱâ Æí¸®ÇÏ°í, ¿¡·¯ ¹× ¹é±×¶ó¿îµå¸¦ ÃÖ¼ÒÈ­ÇÏ¿© Çâ»óµÈ °á°ú¸¦ µµÃâÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº single cell (1 - 10 cells)»Ó ¾Æ´Ï¶ó, ÃßÃâÇÑ ±Ø¼Ò·®(6 - 50 pg)ÀÇ gDNA·ÎºÎÅÍ Whole Genome Amplification (WGA)¸¦ ÁøÇàÇÒ ¼ö ÀÖÀ¸¸ç, ÁõÆøÇÑ DNA´Â PCRÀº ¹°·Ð array CGH analysis¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
PicoPLEX¢ç WGA KitÀº in vitro fertilization(IVF, ü¿Ü¼öÁ¤)½Ã pre-implantation genetic screening and diagnostics (PGS/PGD)¿Í °°Àº °Ë»ç ½Ã À¯¿ëÇÏ°Ô »ç¿ëµÇ°í ÀÖ´Ù.


±×¸² 1. PicoPLEX¢ç WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (PicoPLEX¢ç ±â¼ú °³¿ä º¸±â)


±×¸² 2. Reproducible amplification
Flow-sorted cancer cells were amplified with PicoPLEX single cell WGA technology. Each of the cells (blue lines) amplified at the same rate and resulted in a similar, predictable yield. A pooled sample of five cells (red line) represents a positive control. Samples containing 0 cells (green lines, no template control) show very low background.


±×¸² 3. Reproducible CNVs
Single-blastomere biopsies were amplified using PicoPLEX single cell WGA technology, labeled and hybridized to BlueGnome¡¯s 24 Sure arrays at Genesis Genetics Institute. Note the clear indiciation of CNVs. In 2011 ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX technology.


±×¸² 4. Greater reliability
Locus-specific qPCR was used to quantify 48 loci in independent single-cell libraries. Data shown compares results of DNA from two individual samples amplified with (left to right) PicoPLEX single cell WGA technology, GenomePLEX, and GehomiPhi using 10pg of DNA. More than 90% of the product from the PicoPLEX single cell WGA technology produced identifiable, highly reproducible human sequences.
Applications
  • Copy Number Variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
  • Target enrichment or amplicon enrichment for mutation detection
  • SNP Genotyping
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- Cell Extraction Buffer
- Extraction Enzyme Dilution Buffer
- Cell Extraction Enzyme
- Pre-Amp W Buffer
- Pre-Amp W Enzyme
- Amplification Buffer
- Amplification Enzyme
- Nuclease-Free Water
Citation
PicoPLEX® WGA Kit´Â PicoPLEX® Single Cell WGA Kit·Î ¾÷µ¥ÀÌÆ®µÇ¾úÀ¸¸ç, °¡Àå ÃֽŹöÀüÀº PicoPLEX® Single Cell WGA Kit v3ÀÔ´Ï´Ù. CitationÀÌ µ¿ÀÏÇÕ´Ï´Ù.

Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis
American Journal of Human Genetics    |    2019 Dec 05    |    PubMed ID: 31785788    |    Read Article

Mutated clones driving leukemic transformation are already detectable at the single-cell level in CD34-positive cells in the chronic phase of primary myelofibrosis
NPJ Precision Oncology    |    2021 Feb 04    |    PubMed ID: 33542466    |    Read Article

Investigation of somatic CNVs in brains of synucleinopathy cases using targeted SNCA analysis and single cell sequencing
Acta Neuropathologica Communications    |    2019 Dec 23    |    PubMed ID: 31870437    |    Read Article

Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure
Human Reproduction    |    2019 Dec 01    |    PubMed ID: 31811307    |    Read Article

Binding of a C-type lectin’s coiled-coil domain to the Domeless receptor directly activates the JAK/STAT pathway in the shrimp immune response to bacterial infection
PLoS Pathogens    |    2017 Sep 20    |    PubMed ID: 28931061    |    Read Article

SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit
Horticulture Research    |    2021 Dec 01    |    PubMed ID: 34848692    |    Read Article

Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single
-cell level, et al. Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level
Cancer Science    |    2021 Apr 02    |    PubMed ID: 33484069    |    Read Article

Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC
-chip” for primary lung cancer, et al. Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC-chip” for primary lung cancer
Cancer Science    |    2022 Jan 28    |    PubMed ID: 34964211    |    Read Article

Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
Frontiers in Cell and Developmental Biolo…    |    2021 Dec 03    |    PubMed ID: 34926439    |    Read Article

Single-Circulating Tumor Cell Whole Genome Amplification to Unravel Cancer Heterogeneity and Actionable Biomarkers
International Journal of Molecular Scienc…    |    2022 Jul 29    |    PubMed ID: 35955517    |    Read Article

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices
International Journal of Molecular Scienc…    |    2022 Dec 01    |    PubMed ID: 36499466    |    Read Article

Genomic Analysis of Hematopoietic Stem Cell at the Single-Cell Level: Optimization of Cell Fixation and Whole Genome Amplification (WGA) Protocol
International Journal of Molecular Scienc…    |    2020 Oct 06    |    PubMed ID: 33036143    |    Read Article

Functional Analysis of the Active Site of a Metallo-β-Lactamase Proliferating in Japan
Antimicrobial Agents and Chemotherapy    |    1999 Jul 06    |    PubMed ID: 10952572    |    Read Article

A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples
Scientific Reports    |    2019 Aug 20    |    PubMed ID: 31431640    |    Read Article

Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter
Scientific Reports    |    2019 Dec 27    |    PubMed ID: 31882696    |    Read Article

Kinship analysis on single cells after whole genome amplification
Scientific Reports    |    2020 Sep 04    |    PubMed ID: 32887915    |    Read Article

First detection of TBE virus in ticks and sero-reactivity in goats in a non-endemic region in the southern part of Switzerland (Canton of Ticino).
Ticks and tick-borne diseases    |    2019 Aug 15    |    PubMed ID: 31047827    |    Read Article

Serological Investigation and Genetic Characteristics of Pseudorabies Virus in Hunan Province of China From 2016 to 2020
Frontiers in Veterinary Science    |    2021 Dec 16    |    Read Article

A common variant of CNTNAP2 is associated with sub-threshold autistic traits and intellectual disability
PLoS ONE    |    2021 Dec 13    |    PubMed ID: 34898614    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with Single Circulating Trophoblast (SCT) as one form of cell-based NIPT
PLoS ONE    |    2021 Apr 15    |    PubMed ID: 33857205    |    Read Article

Haplotyping by linked-read sequencing (HLRS) of the genetic disease carriers for preimplantation genetic testing without a proband or relatives
BMC Medical Genomics    |    2020 Aug 20    |    PubMed ID: 32819358    |    Read Article

A novel method for noninvasive diagnosis of monogenic diseases from circulating fetal cells.
Prenatal diagnosis    |    2021 Dec 10    |    PubMed ID: 32673403    |    Read Article

Cell-based non-invasive prenatal diagnosis in a pregnancy at risk of cystic fibrosis.
Prenatal diagnosis    |    2022 Jan 03    |    PubMed ID: 33150588    |    Read Article

Reliable detection of subchromosomal deletions and duplications using cell
-based noninvasive prenatal testing
Prenatal Diagnosis    |    2018 Nov 19    |    PubMed ID: 30357877    |    Read Article

Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification
Malaria Journal    |    2020 May 27    |    PubMed ID: 32460780    |    Read Article

A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells
Micromachines    |    2019 Feb 17    |    PubMed ID: 30781548    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with single circulating trophoblast (SCT) prenatal diagnosis
medRxiv    |    2020 Jun 02    |    Read Article

Intercellular extrachromosomal DNA copy number heterogeneity drives cancer cell state diversity
bioRxiv    |    2023 Jan 21    |    Read Article

Artificial intelligence-driven morphology-based enrichment of malignant cells from body fluid
bioRxiv    |    2023 Jan 25    |    Read Article

>> ³í¹® ´õ º¸±â (¿¬°áµÇ´Â ÆäÀÌÁö ÇÏ´ÜÀ» Âü°íÇØÁÖ¼¼¿ä)

Keyword : Sngle cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation

Whole Genome Amplification (WGA)
>
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplificat..
PicoPLEX¢ç Single Cell WGA Kit v3
>
Whole genome amplification (WGA) from single..
PicoPLEX¢ç Single Cell WGA Kit