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ÀüÁ¦Ç°º¸±â
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NGS °ü·Ã
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±âŸ RNA-Seq
> Target RNA Capture for Illumina¢ç
¿øÇÏ´Â RNA¸¸ capture - Targeted RNA-Seq Library Á¦ÀÛ
Target RNA Capture for Illumina¢ç
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Clontech
635035
SMARTer¢ç Target RNA Capture for Illumina¢ç
12ȸ
(Package)
2,838,000¿ø
Clontech
635036
SMARTer¢ç Target RNA Capture for Illumina¢ç
48ȸ
(Package)
7,792,000¿ø
Clontech
635039
Capture Beads
Each
1,654,000¿ø
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Single cell mRNA-Seq K..
Ultra low input mRNA-S..
High throughput, Autom..
Ultra low input 3¡¯end..
Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
[New]High Input Strand..
Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
High Input Stranded RN..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç WG..
Inputs as low as 10 ng of purified total RNA
Streamlined workflow for specific enrichment of transcripts of interest
Generate Illumina¢ç-ready, full-length sequencing libraries from the enriched DNA
Ligation-independent sequencing adapter addition for library preparation
Á¦Ç°¼³¸í
SMARTer¢ç Target RNA Capture for Illumina¢ç
´Â enriched total RNA·ÎºÎÅÍ cDNA NGS Library Á¦ÀÛÀ» À§ÇÑ complete systemÀÌ´Ù. Targeted RNA-SeqÀº ÀûÀº ¾çÀ¸·Î Á¸ÀçÇÏ´Â ÀϺΠÀü»çü (transcript)ÀÇ sequence coverage¿Í °ËÃâ °µµ¸¦ ³ôÀÏ ¼ö ÀÖÀ¸¹Ç·Î ±âÁ¸ÀÇ ÀüÀå Àü»çü ºÐ¼® (Whole transcriptome amplification (WTA) RNA-Seq)ÀÇ ¿©·¯ °¡Áö ÇÑ°èÁ¡À» ÇØ°áÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ±âÁ¸ÀÇ WTA ¹æ½Ä¿¡ ºñÇÏ¿© ½ÇÇè ¹æ¹ýÀÌ º¸´Ù ´Ü¼øÇÏ¿© ºÐ¼® ºñ¿ëÀ» Àý°¨ÇÒ ¼ö ÀÖ´Ù.
cDNA ÇÕ¼º Àü, ºÐ¼®ÇÏ°íÀÚ ÇÏ´Â RNA Àü»çü¸¸ ƯÀÌÀûÀ¸·Î ³óÃà (Enrichment)ÇÔÀ¸·Î½á chimeric gene fusion, transcript isoform, splice variants µî ½ÃÄö½Ì °úÁ¤¿¡¼ ¼Õ½ÇµÇ°Å³ª ´õ ¸¹Àº ¾çÀÇ sequencing read number¸¦ ¿ä±¸ÇÏ´Â RNA Àü»çüÀÇ Á¤º¸¸¦ È¿°úÀûÀ¸·Î Àâ¾Æ³¾ (capture) ¼ö ÀÖ´Ù. ÀÌ¿¡ µû¶ó NGS µ¥ÀÌÅÍÀÇ signal-to-noise ratioÀÇ ºñÀ²À» °¨¼Ò½Ãų ¼ö ÀÖÀ¸¹Ç·Î º¸´Ù ³ôÀº ¹Î°¨µµ¿Í ºÐ¼®´ÉÀ¸·Î NGS ºÐ¼®À» ½ÃÇàÇÒ ¼ö ÀÖ´Ù.
SMARTer¢ç Target RNA Capture for Illumina¢çÀÇ ½ÇÇè´Ü°è¿¡¼ total RNA »ùÇÃÀº ¿¬±¸ÀÚ°¡ µðÀÚÀÎÇÑ biotinylated DNA probe¿Í total RNA sampleÀ» HybridizationµÈ´Ù. Hybridized RNA-DNA Complex´Â Á¤Á¦ ÈÄ, streptavidinÀÌ ÄÚÆÃµÈ magnetic particles - Capture beads¿¡ ÀÇÇÏ¿© ºÐ¸® (Capture)µÈ´Ù (±×¸² 1.) Capture Beads´Â ´Ü¹éÁú°ú Çٻ꿡 ³·Àº ÈíÂø·ÂÀ» º¸À̱⠶§¹®¿¡ PCR ¶Ç´Â ¿ªÀü»ç ¹ÝÀÀ°ú °°Àº ÃßÈÄ ½ÇÇè ¹ÝÀÀ (downstream reaction)¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â´Ù. Capture beads´Â SMARTer¢ç Target RNA Capture for Illumina¢ç Á¦Ç° ³»¿¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, º°µµ·Î ±¸¸Å °¡´ÉÇÏ´Ù (Code 635039).
º» Á¦Ç°Àº Clontech ƯÇãÀÇ
SMART-Seq¢ç v4 Ultra Low Input Kit for Sequencing
±â¼úÀÇ ¹Î°¨µµ°¡ °áÇյǾî ÀÖ¾î, enriched transcript·ÎºÎÅÍ °íÇ°ÁúÀÇ full-length Cdna¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖÀ¸¸ç 10ngÀÇ RNA·ÎºÎÅÍ Targeted RNA-Seq Library¸¦ Á¦ÀÛ °¡´ÉÇÒ »Ó¸¸ ¾Æ´Ï¶ó Clontech ÀÚ»ç µ¥ÀÌÅÍ¿¡ ÀÇÇϸé 100pgÀÇ total RNA input¿¡¼µµ ¼º°øÀûÀÎ ½ÇÇè°á°ú¸¦ º¸¿´´Ù (
Technical Note È®ÀÎÇϱâ
). º» Á¦Ç°Àº oligo(dT) primingÀ» ÀÌ¿ëÇϸç high-quality RNA sampleÀ» ÇÊ¿ä·Î Çϸç Ãß°¡ÀûÀÎ rRNA Á¦°Å °úÁ¤À̳ª ½Ã¾àÀ» ÇÊ¿ä·Î ÇÏÁö ¾Ê°í Á¦Á¶µÈ NGS Library´Â Illumina¢ç NGS Platform·Î ºÐ¼® °¡´ÉÇÏ´Ù.
±×¸² 1. SMARTer¢ç Target RNA Capture for Illumina¢ç protocol overview.
The protocol through validation is completed over two days, with just 2.5-3 hours of hands-on time and without the need for additional rRNA removal methods or kits.
±×¸² 2. Example electropherogram results from Agilent 2100 Bioanalyzer.
Unless indicated, 10 ng of Control Total RNA was subjected to hybridization with HPRT1 Control Probes, capture with Capture Beads, SMART-Seq v4 cDNA synthesis, and amplification for 20 cycles as described in the protocol.
Panel A
shows a single distinct peak between 1,400-1,650 bp.
Panels B and C
show no product in the negative controls (no RNA and no probes, respectively).
Panel D
shows the cDNA profile resulting from 10 ng of Control Total RNA hybridized to probes for 10 different genes, following amplification for 17 cycles.
Application
- Sequencing library preparation for targeted RNA-seq from purified total RNA
- Illumina¢ç-specific NGS sequencing library production
- Detection of rare gene fusion events at low sequencing depth
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ Âü°íÇϼ¼¿ä)
- SMARTer Target RNA Capture for Illumina Components
- SeqAmp¢â DNA Polymerase
Keyword : Targeted RNA-Seq,Target-seq,Target,RNA-Seq,NGS,SMARTer,SMART-Seq,SMART
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
¿øÇÏ´Â RNA¸¸ capture - Targeted RNA-Seq Libr..
Target RNA Capture for Illumina¢ç