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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > ±âŸ RNA-Seq > Target RNA Capture for Illumina¢ç
¿øÇÏ´Â RNA¸¸ capture - Targeted RNA-Seq Library Á¦ÀÛ

Target RNA Capture for Illumina¢ç

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Clontech
635035
SMARTer¢ç Target RNA Capture for Illumina¢ç
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Clontech
635036
SMARTer¢ç Target RNA Capture for Illumina¢ç
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7,792,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635039
Capture Beads
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  • Inputs as low as 10 ng of purified total RNA
  • Streamlined workflow for specific enrichment of transcripts of interest
  • Generate Illumina¢ç-ready, full-length sequencing libraries from the enriched DNA
  • Ligation-independent sequencing adapter addition for library preparation
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SMARTer¢ç Target RNA Capture for Illumina¢ç´Â enriched total RNA·ÎºÎÅÍ cDNA NGS Library Á¦ÀÛÀ» À§ÇÑ complete systemÀÌ´Ù. Targeted RNA-SeqÀº ÀûÀº ¾çÀ¸·Î Á¸ÀçÇÏ´Â ÀϺΠÀü»çü (transcript)ÀÇ sequence coverage¿Í °ËÃâ °­µµ¸¦ ³ôÀÏ ¼ö ÀÖÀ¸¹Ç·Î ±âÁ¸ÀÇ ÀüÀå Àü»çü ºÐ¼® (Whole transcriptome amplification (WTA) RNA-Seq)ÀÇ ¿©·¯ °¡Áö ÇÑ°èÁ¡À» ÇØ°áÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ±âÁ¸ÀÇ WTA ¹æ½Ä¿¡ ºñÇÏ¿© ½ÇÇè ¹æ¹ýÀÌ º¸´Ù ´Ü¼øÇÏ¿© ºÐ¼® ºñ¿ëÀ» Àý°¨ÇÒ ¼ö ÀÖ´Ù.
cDNA ÇÕ¼º Àü, ºÐ¼®ÇÏ°íÀÚ ÇÏ´Â RNA Àü»çü¸¸ ƯÀÌÀûÀ¸·Î ³óÃà (Enrichment)ÇÔÀ¸·Î½á chimeric gene fusion, transcript isoform, splice variants µî ½ÃÄö½Ì °úÁ¤¿¡¼­ ¼Õ½ÇµÇ°Å³ª ´õ ¸¹Àº ¾çÀÇ sequencing read number¸¦ ¿ä±¸ÇÏ´Â RNA Àü»çüÀÇ Á¤º¸¸¦ È¿°úÀûÀ¸·Î Àâ¾Æ³¾ (capture) ¼ö ÀÖ´Ù. ÀÌ¿¡ µû¶ó NGS µ¥ÀÌÅÍÀÇ signal-to-noise ratioÀÇ ºñÀ²À» °¨¼Ò½Ãų ¼ö ÀÖÀ¸¹Ç·Î º¸´Ù ³ôÀº ¹Î°¨µµ¿Í ºÐ¼®´ÉÀ¸·Î NGS ºÐ¼®À» ½ÃÇàÇÒ ¼ö ÀÖ´Ù.
SMARTer¢ç Target RNA Capture for Illumina¢çÀÇ ½ÇÇè´Ü°è¿¡¼­ total RNA »ùÇÃÀº ¿¬±¸ÀÚ°¡ µðÀÚÀÎÇÑ biotinylated DNA probe¿Í total RNA sampleÀ» HybridizationµÈ´Ù. Hybridized RNA-DNA Complex´Â Á¤Á¦ ÈÄ, streptavidinÀÌ ÄÚÆÃµÈ magnetic particles - Capture beads¿¡ ÀÇÇÏ¿© ºÐ¸® (Capture)µÈ´Ù (±×¸² 1.) Capture Beads´Â ´Ü¹éÁú°ú Çٻ꿡 ³·Àº ÈíÂø·ÂÀ» º¸À̱⠶§¹®¿¡ PCR ¶Ç´Â ¿ªÀü»ç ¹ÝÀÀ°ú °°Àº ÃßÈÄ ½ÇÇè ¹ÝÀÀ (downstream reaction)¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â´Ù. Capture beads´Â SMARTer¢ç Target RNA Capture for Illumina¢ç Á¦Ç° ³»¿¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, º°µµ·Î ±¸¸Å °¡´ÉÇÏ´Ù (Code 635039).
º» Á¦Ç°Àº Clontech ƯÇãÀÇ SMART-Seq¢ç v4 Ultra Low Input Kit for Sequencing ±â¼úÀÇ ¹Î°¨µµ°¡ °áÇյǾî ÀÖ¾î, enriched transcript·ÎºÎÅÍ °íÇ°ÁúÀÇ full-length Cdna¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖÀ¸¸ç 10ngÀÇ RNA·ÎºÎÅÍ Targeted RNA-Seq Library¸¦ Á¦ÀÛ °¡´ÉÇÒ »Ó¸¸ ¾Æ´Ï¶ó Clontech ÀÚ»ç µ¥ÀÌÅÍ¿¡ ÀÇÇϸé 100pgÀÇ total RNA input¿¡¼­µµ ¼º°øÀûÀÎ ½ÇÇè°á°ú¸¦ º¸¿´´Ù (Technical Note È®ÀÎÇϱâ). º» Á¦Ç°Àº oligo(dT) primingÀ» ÀÌ¿ëÇϸç high-quality RNA sampleÀ» ÇÊ¿ä·Î Çϸç Ãß°¡ÀûÀÎ rRNA Á¦°Å °úÁ¤À̳ª ½Ã¾àÀ» ÇÊ¿ä·Î ÇÏÁö ¾Ê°í Á¦Á¶µÈ NGS Library´Â Illumina¢ç NGS Platform·Î ºÐ¼® °¡´ÉÇÏ´Ù.


±×¸² 1. SMARTer¢ç Target RNA Capture for Illumina¢ç protocol overview. The protocol through validation is completed over two days, with just 2.5-3 hours of hands-on time and without the need for additional rRNA removal methods or kits.


±×¸² 2. Example electropherogram results from Agilent 2100 Bioanalyzer. Unless indicated, 10 ng of Control Total RNA was subjected to hybridization with HPRT1 Control Probes, capture with Capture Beads, SMART-Seq v4 cDNA synthesis, and amplification for 20 cycles as described in the protocol. Panel A shows a single distinct peak between 1,400-1,650 bp. Panels B and C show no product in the negative controls (no RNA and no probes, respectively). Panel D shows the cDNA profile resulting from 10 ng of Control Total RNA hybridized to probes for 10 different genes, following amplification for 17 cycles.
Application
- Sequencing library preparation for targeted RNA-seq from purified total RNA
- Illumina¢ç-specific NGS sequencing library production
- Detection of rare gene fusion events at low sequencing depth
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ Âü°íÇϼ¼¿ä)
- SMARTer Target RNA Capture for Illumina Components
- SeqAmp¢â DNA Polymerase

Keyword : Targeted RNA-Seq,Target-seq,Target,RNA-Seq,NGS,SMARTer,SMART-Seq,SMART

±âŸ RNA-Seq
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¿øÇÏ´Â RNA¸¸ capture - Targeted RNA-Seq Libr..
Target RNA Capture for Illumina¢ç