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Home > 전제품보기 > NGS 관련 > Low Input DNA-Seq > ThruPLEX® DNA-seq Kit
All-purpose low input DNA library preparation for Illumina® NGS platform

ThruPLEX® DNA-seq Kit

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
R400674
ThruPLEX® DNA-Seq Kit
관련학술 구매하기 라이선스 
24회
가격문의   제조사 페이지로 바로가기 x102090
Clontech
R400675
ThruPLEX® DNA-Seq Kit
관련학술 구매하기 라이선스 
48회
가격문의   제조사 페이지로 바로가기
Clontech
R400676
ThruPLEX® DNA-Seq Kit
관련학술 구매하기 라이선스 
96회
가격문의   제조사 페이지로 바로가기
Clontech
R400677
ThruPLEX® DNA-Seq Kit
관련학술 구매하기 라이선스 
480회
가격문의   제조사 페이지로 바로가기


* 잠깐, 첫 구매인가요? 다카라코리아 고객지원센터 (Tel. 02-2081-2510)로 연락주세요!

* 본 제품은 기존 ThruPLEX® DNA-seq Kit (Code R400523)의 업그레이드 버전 제품이며, indexing primer kit가 포함되어있지 않습니다.
  • High performance from low inputs - 미량의 DNA 샘플 (50 pg ~ 50 ng)로부터 Illumina® NGS library 제작
  • Fast and simple workflow - 튜브 이동 없이 단일튜브 (single-tube or single-well)에서 세 번의 실험 과정 (3-step)으로 제작 완료 (library 제작 시간 : 약 2시간)
  • Compatible with major target enrichment platforms - 주요 Target Enrichment 플랫폼에 적용 가능 - Agilent SureSelect®, Roche Nimblegen® SeqCap® EZ, and IDT xGen® Lockdown® probes 외
    ※ DNA Target Enrichment Protocol 자세히 보기
제품설명
ThruPLEX® DNA-seq Kit은 ThruPLEX® 기술을 이용하여 극소량의 DNA로부터 Illumina® NGS Library를 제작할 수 있는 제품이다. 본 제품을 이용하면 불과 50 pg 수준의 극소량 DNA로 최대 384 multiplexing에 대응 가능한 Illumina® NGS Library를 제작 가능하다. 본 제품은 샘플 종류에 상관없이 fragmentation된 DNA라면 모두 적용이 가능하다 (Genomic, biofluids such as cell-free DNA, DNA from FFPE materials, cDNA).
ThruPLEX® DNA-seq Kit을 이용하면 단일튜브에서 튜브 이동 없이 2시간 만에 NGS Library를 제작 가능하다. ThruPLEX® DNA-seq Kit은 다양한 DNA-seq application과 RNA-seq을 위한 cDNA 그리고 ChIP-seq 등에 사용될 수 있으며, 주요 target enrichment platform protocol도 함께 제공되고 있다 (※ DNA Target Enrichment Protocol 자세히 보기).

본 제품은 기존 ThruPLEX® DNA-Seq Kit의 업그레이드 제품이며, Illumina index는 포함하고 있지 않다. 다카라바이오에서 제공하는 모든 DNA Index Kits를 적용 가능하다 ( DNA Index Kit 바로가기)


그림 1. ThruPLEX DNA-Seq single-tube library preparation workflow. The ThruPLEX DNA-Seq workflow consists of three simple steps that take place in the same PCR tube or well, eliminating the need to purify and transfer the sample material.)


그림 2. ThruPLEX®  기술 (자세히 보기)
Step 1. 단편화된 double stranded DNA 말단을 고효율로 Repair한다
Step 2. DNA 절편의 5’ 말단에 Stem-Loop adapter가 ligation되며, 3’ 말단에는 nick을 남긴다. 이후 extension 과정을 통해 Stem-Loop adapter의 일부가 제거된다.
Stem-Loop adapter는 노출된 단일가닥이 없고, 5’ 말단이 block되어 있어 adapter간의 dimer 형성이 억제될 뿐만 아니라 서열 분석에서의 background를 감소시킬 수 있다.
Step 3. Illumina® index가 포함된 primer로 증폭하여 Illumina® NGS 분석용 Llibrary 제작을 완료한다.

표 1. DNA Sample Requirements for ThruPLEX® DNA-seq Kit
DNA Sample Requirements

Nucleic Acid

Fragmented double-stranded DNA or cDNA

Source

Cells, plasma, urine, other biofluids, FFPE, tissues, fresh tissues, frozen tissues

Type

Mechanically sheared; enzymatically fragmented;
ChIP-DNA; low-molecular weight cell-free DNA

Molecular Weight

< 1,000 bp

Input Amount

50 pg ~ 50 ng

Input Volume

10 ㎕


* 자세한 내용은 사용자 매뉴얼을 참고하세요.
Application
* 링크를 클릭하시면 ThruPLEX® DNA-seq Kit의 다양한 실험 데이터를 확인하실 수 있습니다.
Application

Technical Note

ChIP-Seq

Low cell number ChIP-seq using the ThruPLEX DNA-seq kit as a tool for epigenetic profiling

FFPE DNA

A streamlined solution for generating high-quality NGS libraries from FFPE DNA samples

Low input exome-seq

Low-input whole-exome sequencing using ThruPLEX technology and Agilent SureSelect Target Enrichment Systems

Low volume DNA shearing

Using Covaris microTUBE-15 to shear samples for ThruPLEX library preparation

구성품 (자세한 내용은 CoA를 참조하세요)
ThruPLEX® DNA-seq Kit
  • Template Preparation D Buffer
  • Template Preparation D Enzyme
  • Library Synthesis D Buffer
  • Library Synthesis D Enzyme
  • Library Amplification D Buffer
  • Library Amplification Enzyme
  • Nuclease-Free Water
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Keyword : low input,DNA-seq,FFPE,cfDNA,cell-free DNA,DNA fragment,Enzymatic fragmentation,NGS,Next generation sequencing,NovaSeq,UDI,Unique dual index,Rubicon,루비콘,Cancer research,암연구,Cancer

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