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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > mRNA-Seq (Single cell) > [New]Ultra low input mRNA-Seq Kit
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ÃÖ°­ÀÚ

[New]Ultra low input mRNA-Seq Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
634762
SMART-Seq¢ç mRNA LP (with UMIs)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
(Package)
2,880,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x340780
Clontech
634765
SMART-Seq¢ç mRNA LP (with UMIs)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
(Package)
9,558,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634766
SMART-Seq¢ç mRNA LP (with UMIs)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384ȸ
(96ȸx4)
34,407,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634768
SMART-Seq¢ç mRNA LP
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
(Package)
2,698,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x341117
Clontech
634769
SMART-Seq¢ç mRNA LP
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
(Package)
8,837,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634771
SMART-Seq¢ç mRNA LP
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384ȸ
(96ȸx4)
31,813,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634772
SMART-Seq¢ç mRNA
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
(Package)
2,163,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x341098
Clontech
634773
SMART-Seq¢ç mRNA
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
(Package)
6,693,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634770
SMART-Seq¢ç mRNA
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384ȸ (630773 x 4)
24,095,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

¡Ø º¯°æ»çÇ×
‘SMART-Seq® mRNA LP’¿Í ‘SMART-Seq® mRNA’´Â °¢°¢ ‘SMART-Seq® v4 PLUS’¿Í ‘SMART-Seq® v4 Ultra Low Input RNA Kit for Sequencing’ÀÇ µ¿ÀÏÁ¦Ç°À¸·Î, Á¦Ç°¸í º¯°æ ¹× Á¦Ç°°£ ±¸¼ºÇ° Á¾·ù¸¦ ÅëÀÏÇÏ°í ¹ÝÀÀ ´ç ´Ü°¡¸¦ ÀÎÇÏÇÏ¿´½À´Ï´Ù
ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÀ½ ¾È³»ÆäÀÌÁö¸¦ Âü°íÇϼ¼¿ä! Á¦Ç° ¼±ÅÃÀÌ ½¬¿öÁý´Ï´Ù.
>> RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸°Ô ¹Ù²ò´Ï´Ù

¡Ø ¾÷µ¥ÀÌÆ® ³»¿ë
¾Æ·¡ÀÇ ¾÷µ¥ÀÌÆ® »çÇ×Àº ½ÇÇè°úÁ¤À̳ª Á¦Ç° È¿À²¿¡ ¿µÇâÀÌ ¾ø½À´Ï´Ù.  

  • Total RNA Input range°¡ 10 pg-10 ng¿¡¼­ 10 pg-100 ng·Î È®Àå
  • SeqAmp PCR buffer´ë½Å SeqAmp CB PCR buffer·Î Á¦°øµÇ¾î, DNA cleanup °úÁ¤ÀÇ bead pelleting°ú resuspension Çâ»ó
  • Á¦Ç°»ç¿ë À¯¿¬¼ºÀ» À§ÇØ index°¡ kit¿¡ Æ÷ÇԵǾî ÀÖÁö ¾ÊÀ¸¸ç, º°µµ·Î ±¸¸Å°¡ °¡´É >> ȣȯµÇ´Â Unique Dual Index kits 
  • Afa I, 10X Afa I Buffer, 0.1% BSA ¹ÌÁ¦°ø (½ÇÇè°úÁ¤ Áß ÇÊ¿äÇÏÁö ¾ÊÀ½)
  • 10 pg ¼öÁØÀÇ ±Ø¼Ò·® total RNA¿¡µµ Àû¿ë °¡´É
  • Unparalleled sensitivity and reproducibility
  • Single-tube workflows - cDNA ÇÕ¼ººÎÅÍ library Á¦ÀÛ °úÁ¤À» °¢°¢ single tube¿¡¼­ ÁøÇàÇÒ ¼ö ÀÖ¾î, »ùÇà ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼­ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù ÃÖ¼ÒÈ­
  • High-quality RNA-seq data - total RNA·ÎºÎÅÍ °¡Àå ¸¹Àº ¼öÀÇ À¯ÀüÀÚ ºÐ¼®
  • Increase your sequencing power– ¸¹Àº ¼öÀÇ »ùÇÃÀ» Çѹø¿¡ ºÐ¼® ½Ã, Unique dual index (UDI)¸¦ »ç¿ëÇÏ¿© index hoping ¾øÀÌ multiplexing ºÐ¼®ÀÌ °¡´É
  • High library yield - ³ôÀº yieldÀÇ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ¾î,NovaSeq™ system°ú °°Àº high-throughput sequencer¿¡ Àû¿ëÇϰųª, ´ÙȸºÐ¼®ÀÌ °¡´É
Á¦Ç°¼³¸í
SMART-Seq® mRNA Á¦Ç°À» ÀÌ¿ëÇϸé, 10 pg–100 ng total RNA ¶Ç´Â 1-1,000°³ÀÇ ¼¼Æ÷¿¡¼­ RNA ÃßÃâ °úÁ¤ ¾øÀÌ, oligo(dT) primer¸¦ ÀÌ¿ëÇÑ °íÇ°ÁúÀÇ full-length cDNA¸¦ Á¦Á¶ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº SMART® (Switching Mechanism at 5' End of RNA Template) ±â¼ú¿¡ Locked Nucleic Acid (LNA) ±â¼úÀ» Á¢¸ñÇÔÀ¸·Î½á template switchingÀÇ È¿À²À» ´õ¿í Çâ»ó½ÃÄ×´Ù (±×¸²1). µû¶ó¼­, ÀÌÀü ¼¼´ëÀÇ Á¦Ç°º¸´Ù ´õ¿í ¸¹Àº ¼öÀÇ À¯ÀüÀÚ¸¦ °ËÃâÇÒ ¼ö ÀÖ¾î Àü¹ÝÀûÀÎ Àü»çü ¿¬±¸ (full transcriptome analysis)¿¡ ÀÖ¾î ¸Å¿ì À¯¿ëÇÏ´Ù. SMART-Seq® mRNA´Â ³ôÀº ÀçÇö¼º°ú gene-body coverage »Ó ¾Æ´Ï¶ó GC-rich Àü»çüµµ Á¤È®ÇÏ°Ô cDNA·Î ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
SMART-Seq mRNA® LP¿¡ Æ÷ÇԵǾî ÀÖ´Â library preparation kit´Â enzymatic fragmentation¿Í stem-loop adaptor·Î 2½Ã°£ ³»¿¡ single tube¿¡¼­ º°µµÀÇ purification °úÁ¤ ¾øÀÌ NGS library±îÁö Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. µû¶ó¼­ »ùÇà ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼­ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù¸¦ ÃÖ¼ÒÈ­ÇÒ ¼ö ÀÖ´Ù. 
ÀÌ °úÁ¤À» ÅëÇØ Á¦ÀÛµÈ library´Â SMART-Seq2 µî ±âÁ¸ ¹æ¹ý ¹× ´ç»ç ±âÁ¸ Á¦Ç° (SMART-Seq® v3)¿¡ ºñÇØ ÀçÇö¼ºÀÌ ³ô°í, ÈξÀ ¿ì¼öÇÒ »Ó ¾Æ´Ï¶ó, ³ôÀº yield·Î Á¦À۵Ǿî high-throughput sequencer¿¡ Àû¿ëÇϰųª, ÇѹøÀÇ ¹ÝÀÀÀ¸·Î ¿©·¯ Â÷·Ê ¹Ýº¹ ºÐ¼®ÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ ±âÁ¸ Á¦Ç°ÀÎ SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing, SMART-Seq® v4 PLUS Kit ¸¸Å­ÀÇ ³ôÀº °¨µµ¸¦ °¡Áø´Ù.
SMART-Seq mRNA LP (with UMIs)´Â SMART® ¹æ¹ýÀ¸·Î oligo¿¡ Unique Molecular Identifiers (UMI)s¸¦ ºÎ°¡ÇÔÀ¸·Î½á PCR errors, duplicates, amplification biases¸¦ º¸Á¤ÇÑ´Ù.

SMART-Seq mRNA® LP, SMART-Seq® mRNA LP (with UMIs)´Â »ùÇÿ¡¼­ cDNA ÇÕ¼º, library prep¹× ¹«·á Cogent NGS »ý¹°Á¤º¸ÇÐ ÆÄÀÌÇÁ¶óÀÎÀ» ÅëÇÑ µ¥ÀÌÅÍ ºÐ¼®¿¡ À̸£±â±îÁö end-to-end solutionÀ» Á¦°øÇÑ´Ù.

Cogent NGS ¼ÒÇÁÆ®¿þ¾î´Â ¾Æ·¡ ÆäÀÌÁö¿¡¼­ ´Ù¿î·Îµå ¹ÞÀ» ¼ö ÀÖ´Ù.
Cogent NGS Discovery Software(takarabio.com)
Cogent NGS Analysis Pipeline(takarabio.com)


±×¸² 1. SMART±â¼úÀ» ÀÌ¿ëÇÑ cDNA ÇÕ¼º °úÁ¤


±×¸²2. SMART-Seq mRNA® LP¸¦ ÀÌ¿ëÇÑ Single tube library prep ¿öÅ©Ç÷οì
Library prep °úÁ¤¿¡ UDI (Unique Dual Index) kit (Code 634752 ¿Ü, º°µµ ÆǸÅ)¸¦ ÇÔ²² »ç¿ëÇϸé ÃÖ´ë 384°³ÀÇ multiplexed Illumina-ready sequencing libraries¸¦ »ý¼ºÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº high-throughput »ùÇà 󸮿ë liquid handlers¸¦ À§ÇÑ »ç¿ë·® ÃÖÀûÈ­ (miniaturization) ¹× ÀÚµ¿È­ (automation) ¿¡µµ ȣȯµÈ´Ù. Degraded samples¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â random priming ¹æ¹ýÀ» ¼±È£ÇÒ °æ¿ì, Stranded total RNA-Seq kit (Code 634485 ¿Ü)¸¦ ±ÇÀåÇÑ´Ù.


±×¸²3. Unique Molecular Identifier (UMI)¸¦ žÀçÇÑ SMART® ¹ýÀÇ cDNA ÇÕ¼º ¿öÅ©Ç÷οì SMART-Seq® mRNA LP (with UMIs) Á¦Ç°À» »ç¿ë½Ã, UMIs ºÎ°¡·Î Á¤È®µµ¿Í ½Å·Úµµ¸¦ ´õ ³ôÀÏ ¼ö ÀÖ´Ù.


±×¸²4. Single-tube ¹ÝÀÀÀ» ÅëÇÑ °£ÆíÇÑ library perp


±×¸²5. CongentDS À¥ ÀÎÅÍÆäÀ̽ºÀÇ UMAP ºÐ¼® Â÷Æ® ¿¹½Ã. Chart display¸¦ ¼öÁ¤Çϱâ À§ÇÑ user controlÀÌ scatterplot °á°ú¿Í ÇÔ²² º¸¿©Áø´Ù.
Àû¿ë
±Ø¼Ò·® »ùÇ÷κÎÅÍ full-length transcriptome ºÐ¼® (transcript isoforms, gene fusions, point mutations µî)À» À§ÇØ, cDNA ÇÕ¼º °úÁ¤¿¡¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù (SMART-Seq mRNA ÀÌ¿ë).
SMART-Seq® mRNA·Î ÇÕ¼ºÇÑ cDNA´Â SMART-Seq® Library Prep kit (SMART-Seq® mRNA LP ³» Æ÷ÇÔ, º°µµ ±¸¸Å ºÒ°¡)¸¦ ÀÌ¿ëÇÏ¿© Illumina® ºÐ¼®¿ë sequencing library·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis¸¦ ÂüÁ¶ÇϽʽÿÀ.

Keyword :

mRNA-Seq (Single cell)
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