´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > [Legacy] NGS Products > (Legacy) PicoPLEX¢ç WGA Kit
Single cell, 10pg DNA - Whole Genome Amplification (WGA)

(Legacy) PicoPLEX¢ç WGA Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
R30050
PicoPLEX¢ç WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
50 ȸ
°¡°Ý¹®ÀÇ   RB3050 Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x99914

  • Single cell, ±Ø¼Ò·® gDNA·ÎºÎÅÍ ¶óÀ̺귯¸® ±¸ÃàÀ» À§ÇÑ Whole Genome Amplification (WGA) Kit
  • Single cell ¶Ç´Â 6pgÀÇ gDNA·ÎºÎÅÍ ¾ÈÁ¤ÀûÀÎ ÁõÆø °á°ú
  • ³ôÀº ÀçÇö¼ºÀÇ allele representation
  • WGA ±â¼ú·Î ÁõÆøÇÑ DNA´Â NGS, Microarray, PCR µî ´Ù¾çÇÑ ¹æ¹ýÀ¸·Î ºÐ¼®
Á¦Ç°¼³¸í
PicoPLEX¢ç WGA KitÀº ´ÜÀÏ ¼¼Æ÷(single cell)·Î ºÎÅÍ ³ôÀº ÀçÇö¼ºÀ» °¡Áø ¶óÀ̺귯¸®¸¦ ¸¸µé±â À§ÇØ DNA¸¦ ÁõÆøÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°ÀÇ ±âº»ÀÌ µÇ´Â °íÀ¯ÀÇ ±â¼ú(ƯÇã ±â¼ú), PicoPLEX¢ç ±â¼úÀº single copy genomic DNAÀÇ ÁõÆø¿¡ ÃÖÀûÈ­µÇ¾î ÀÖ´Ù. ÇϳªÀÇ tube¿¡¼­ ¹ÝÀÀÇÏ´Â single tube reactionÀ¸·Î »ç¿ëÇϱâ Æí¸®ÇÏ°í, ¿¡·¯¸¦ ÃÖ¼ÒÈ­ ÇÒ ¼ö ÀÖÀ¸¸ç, ¹é±×¶ó¿îµå¸¦ ÃÖ¼ÒÈ­ÇÏ¿© Çâ»óµÈ °á°ú¸¦ µµÃâÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº single cell (1 - 10 cells)»Ó ¾Æ´Ï¶ó, ÃßÃâÇÑ ±Ø¼Ò·®(6 - 50 pg)ÀÇ gDNA·ÎºÎÅÍ Whole Genome Amplification (WGA)¸¦ ÁøÇàÇÒ ¼ö ÀÖÀ¸¸ç, ÁõÆøÇÑ DNA´Â PCRÀº ¹°·Ð array CGH analysis¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
PicoPLEX¢ç WGA KitÀº in vitro fertilization(IVF, ü¿Ü¼öÁ¤)½Ã pre-implantation genetic screening and diagnostics (PGS/PGD)¿Í °°Àº °Ë»ç ½Ã À¯¿ëÇÏ°Ô »ç¿ëµÇ°í ÀÖ´Ù.


[±×¸² 1] PicoPLEX¢ç WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (PicoPLEX¢ç ±â¼ú °³¿ä º¸±â)


[±×¸² 2] PicoPLEX¢ç WGA Kit - Single tube workflow


[±×¸²3] Amplification of Single Cells is Reproducible
Flow-sorted cancer cells were amplified with the PicoPLEX¢ç WGA Kit. Each of the cells (blue lines) amplified at the same rate, and resulted in a similar, predictable yield. The sample containing 0 cells (green lines; no template control) shows very low background.


[±×¸²4] Reproducible Copy Number Variation(CNV) data
Single-blastomere biopsies were amplified using PicoPLEX¢ç WGA Kit, labeled and hybridized to BlueGnome¡¯s 24Sure arrays at Genesis Genetics Institute. Note clear indication of CNVs. In 2011, ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX¢ç technology.
º¸°ü
Store at -20 ¡ÆC.
Guarantee for 12 months at -20¡ÆC in a constant temperature freezer.
³»¿ë : 50 ȸºÐ

Component Name

Tube Cap Color

Cell Extraction Buffer

Green

Extraction Enzyme Dilution Buffer

Violet

Cell Extraction Enzyme

Yellow

Pre-Amp Buffer

Red

Pre-Amp Enzyme

White

Amplification Buffer

Orange

Amplification Enzyme

Blue

Nuclease-Free Water

Clear

Citation
PicoPLEX® WGA Kit´Â PicoPLEX® Single Cell WGA Kit·Î ¾÷µ¥ÀÌÆ®µÇ¾úÀ¸¸ç, °¡Àå ÃֽŹöÀüÀº PicoPLEX® Single Cell WGA Kit v3ÀÔ´Ï´Ù. CitationÀÌ µ¿ÀÏÇÕ´Ï´Ù.

Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis
American Journal of Human Genetics    |    2019 Dec 05    |    PubMed ID: 31785788    |    Read Article

Mutated clones driving leukemic transformation are already detectable at the single-cell level in CD34-positive cells in the chronic phase of primary myelofibrosis
NPJ Precision Oncology    |    2021 Feb 04    |    PubMed ID: 33542466    |    Read Article

Investigation of somatic CNVs in brains of synucleinopathy cases using targeted SNCA analysis and single cell sequencing
Acta Neuropathologica Communications    |    2019 Dec 23    |    PubMed ID: 31870437    |    Read Article

Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure
Human Reproduction    |    2019 Dec 01    |    PubMed ID: 31811307    |    Read Article

Binding of a C-type lectin’s coiled-coil domain to the Domeless receptor directly activates the JAK/STAT pathway in the shrimp immune response to bacterial infection
PLoS Pathogens    |    2017 Sep 20    |    PubMed ID: 28931061    |    Read Article

SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit
Horticulture Research    |    2021 Dec 01    |    PubMed ID: 34848692    |    Read Article

Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single
-cell level, et al. Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level
Cancer Science    |    2021 Apr 02    |    PubMed ID: 33484069    |    Read Article

Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC
-chip” for primary lung cancer, et al. Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC-chip” for primary lung cancer
Cancer Science    |    2022 Jan 28    |    PubMed ID: 34964211    |    Read Article

Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
Frontiers in Cell and Developmental Biolo…    |    2021 Dec 03    |    PubMed ID: 34926439    |    Read Article

Single-Circulating Tumor Cell Whole Genome Amplification to Unravel Cancer Heterogeneity and Actionable Biomarkers
International Journal of Molecular Scienc…    |    2022 Jul 29    |    PubMed ID: 35955517    |    Read Article

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices
International Journal of Molecular Scienc…    |    2022 Dec 01    |    PubMed ID: 36499466    |    Read Article

Genomic Analysis of Hematopoietic Stem Cell at the Single-Cell Level: Optimization of Cell Fixation and Whole Genome Amplification (WGA) Protocol
International Journal of Molecular Scienc…    |    2020 Oct 06    |    PubMed ID: 33036143    |    Read Article

Functional Analysis of the Active Site of a Metallo-β-Lactamase Proliferating in Japan
Antimicrobial Agents and Chemotherapy    |    1999 Jul 06    |    PubMed ID: 10952572    |    Read Article

A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples
Scientific Reports    |    2019 Aug 20    |    PubMed ID: 31431640    |    Read Article

Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter
Scientific Reports    |    2019 Dec 27    |    PubMed ID: 31882696    |    Read Article

Kinship analysis on single cells after whole genome amplification
Scientific Reports    |    2020 Sep 04    |    PubMed ID: 32887915    |    Read Article

First detection of TBE virus in ticks and sero-reactivity in goats in a non-endemic region in the southern part of Switzerland (Canton of Ticino).
Ticks and tick-borne diseases    |    2019 Aug 15    |    PubMed ID: 31047827    |    Read Article

Serological Investigation and Genetic Characteristics of Pseudorabies Virus in Hunan Province of China From 2016 to 2020
Frontiers in Veterinary Science    |    2021 Dec 16    |    Read Article

A common variant of CNTNAP2 is associated with sub-threshold autistic traits and intellectual disability
PLoS ONE    |    2021 Dec 13    |    PubMed ID: 34898614    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with Single Circulating Trophoblast (SCT) as one form of cell-based NIPT
PLoS ONE    |    2021 Apr 15    |    PubMed ID: 33857205    |    Read Article

Haplotyping by linked-read sequencing (HLRS) of the genetic disease carriers for preimplantation genetic testing without a proband or relatives
BMC Medical Genomics    |    2020 Aug 20    |    PubMed ID: 32819358    |    Read Article

A novel method for noninvasive diagnosis of monogenic diseases from circulating fetal cells.
Prenatal diagnosis    |    2021 Dec 10    |    PubMed ID: 32673403    |    Read Article

Cell-based non-invasive prenatal diagnosis in a pregnancy at risk of cystic fibrosis.
Prenatal diagnosis    |    2022 Jan 03    |    PubMed ID: 33150588    |    Read Article

Reliable detection of subchromosomal deletions and duplications using cell
-based noninvasive prenatal testing
Prenatal Diagnosis    |    2018 Nov 19    |    PubMed ID: 30357877    |    Read Article

Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification
Malaria Journal    |    2020 May 27    |    PubMed ID: 32460780    |    Read Article

A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells
Micromachines    |    2019 Feb 17    |    PubMed ID: 30781548    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with single circulating trophoblast (SCT) prenatal diagnosis
medRxiv    |    2020 Jun 02    |    Read Article

Intercellular extrachromosomal DNA copy number heterogeneity drives cancer cell state diversity
bioRxiv    |    2023 Jan 21    |    Read Article

Artificial intelligence-driven morphology-based enrichment of malignant cells from body fluid
bioRxiv    |    2023 Jan 25    |    Read Article

>> ³í¹® ´õ º¸±â (¿¬°áµÇ´Â ÆäÀÌÁö ÇÏ´ÜÀ» Âü°íÇØÁÖ¼¼¿ä)

Keyword :

[Legacy] NGS Products
>
TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ Full-length seque..
(Legacy) Human TCR a/b Profiling Kit
>
Single cell, 10pg DNA - Whole Genome Amplifi..
(Legacy) PicoPLEX¢ç WGA Kit