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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS ºÐ¼®¿ë PCR È¿¼Ò > PrimeSTAR¢ç GXL DNA Polymerase

PrimeSTAR¢ç GXL DNA Polymerase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R050A
PrimeSTAR¢ç GXL DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
250 U
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R050A_e.v1906Da.pdf
Takara
R050B
PrimeSTAR¢ç GXL DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (R050A x 4)
1,016,000¿ø 



* NGS¸¦ À§ÇÑ Long-range PCR¿¡µµ ÃÖÀûÀÎ GXL - Àû¿ëÀÚ·á º¸±â Ŭ¸¯!
* HLA genotypingÀ» À§ÇÑ multiplex PCR - Àû¿ëÀÚ·á º¸±â Ŭ¸¯!
  • ¹ü¿ë¼º ³ôÀº High Fidelity PCR È¿¼ÒÀÇ Ç¥ÁØ
  • µ¶ÀÚÀûÀÎ ½ÅÀåÀÎÀÚ(elongation factor)¸¦ ÷°¡ÇÏ¿© long fragment ÁõÆø ½ÇÇö (human gDNA ~30 kb)
  • GC rich targetµµ È¿À²ÀûÀ¸·Î ÁõÆø
  • PCR È¿¼Ò 2¹è ÷°¡·Î Fast PCR °¡´É (10ÃÊ/kb)
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ¾÷±×·¹À̵åµÈ PrimeSTAR HS DNA Polymerase¿¡ ÇÑÃþ ´õ µ¶ÀÚÀûÀÎ ½ÅÀåÀÎÀÚ (elongation factor)¸¦ ÷°¡ÇÏ¿©, PCR È¿À²À» ºñ¾àÀûÀ¸·Î Çâ»ó½ÃŲ ȹ±âÀûÀÎ High Fidelity PCR È¿¼ÒÀÌ´Ù.
¸Å¿ì ³ôÀº Á¤È®µµ¸¦ À¯ÁöÇϸ鼭 Áö±Ý±îÁöÀÇ High Fidelity PCR È¿¼Ò¿¡¼­´Â º¼ ¼ö ¾ø¾ú´ø ¶Ù¾î³­ ½ÅÀ强À» °¡Áö°í ÀÖ¾î, 30kb ÀÌ»óÀÇ long fragment ÁõÆøÀÌ °¡´ÉÇÏ´Ù. ÁõÆø¿¡ ¾î·Á¿òÀ» °Þ¾ú´ø GC-rich template¿¡ ´ëÇؼ­µµ ¹öÆÛÀÇ º¯°æÀ̳ª Ưº°ÇÑ ¹ÝÀÀ Á¶°ÇÀÇ ¼³Á¤ ¾øÀÌ, °£´ÜÇÏ°Ô ÁõÆø ¼º°øÀ²À» ³ôÀÏ ¼ö ÀÖ´Ù.
¶Ç, ±âÁ¸ÀÇ High Fidelity PCR È¿¼Ò »ç¿ë½Ã ¹ÝÀÀÀÌ ÀúÇصDZ⠽¬¿ü´ø ´Ù·® ÇÙ»ê (template DNA °ú·® ÷°¡)À» Æ÷ÇÔÇÑ ¹ÝÀÀ¿¡¼­µµ template·®ÀÇ Çã¿ë ¹üÀ§°¡ ¸Å¿ì ³Ð¾îÁ®, ¹ßÇö·®ÀÌ ¸Å¿ì Àû¾î template cDNA¸¦ °ú·®À¸·Î ÷°¡ÇÒ ¼ö¹Û¿¡ ¾ø´Â Àú¹ßÇö À¯ÀüÀÚ °ËÃ⠵ ¿ëÀÌÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ, º» È¿¼Ò´Â »ó¿Â¿¡¼­ DNA Polymerase È°¼º ¹× 3¡¯ ¡æ 5¡¯ exonuclease È°¼ºÀ» ¾ïÁ¦ÇÏ´Â ´ÜÀÏ Å¬·Ð Ç×ü¸¦ ÀÌ¿ëÇÑ hot start versionÀÇ PCR È¿¼ÒÀÌ´Ù.
½ÅÀå½Ã°£ 1 min/kbÀÇ PCR Á¶°ÇÀ» Ç¥ÁØ ¹ÝÀÀÁ¶°ÇÀ¸·Î ¸í½ÃÇÏ°í ÀÖÁö¸¸, È¿¼Ò¸¦ 2¹è ÷°¡ÇÏ¸é ½ÅÀå½Ã°£ 10ÃÊ/kbÀÇ °í¼Ó PCR·Î ´Ù¾çÇÑ Å¸°Ù¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë (200 ȸ, 50 §¡ ¹ÝÀÀ½Ã)
1. PrimeSTAR GXL DNA Polymerase (1.25 U/§¡)

200 §¡

2. 5¡¿PrimeSTAR GXL Buffer (Mg2+ plus)*1

1 §¢¡¿2

3. dNTP Mixture (°¢ 2.5 mM)

800 §¡

*1 Mg2+ ³óµµ´Â 5 mM (5¡¿)

º¸Á¸
-20¡É
PCR »ê¹°¿¡ ´ëÇÏ¿©
PrimeSTAR¢ç GXL DNA Polymerase¸¦ ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº ÆòÈ° ¸»´Ü (Blunt End)ÀÌ´Ù.
µû¶ó¼­ T-vector¿¡ Ŭ·Î´× ÇÏ´Â °æ¿ì, PCR »ê¹°ÀÇ 3' ¸»´Ü¿¡ dA¸¦ ºÎ°¡ÇØ¾ß Çϸç, Mighty TA-cloning Reagent Set for PrimeSTAR¢ç(Code 6019)¸¦ ÀÌ¿ëÇÏ¿© A-tailing ¹× TA cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
PCR »ê¹°À» ±×´ë·Î (ÇÊ¿ä¿¡ µû¶ó¼­ ÀλêÈ­ÇÏ¿©) ÆòÈ° ¸»´Ü º¤ÅÍ¿¡ Ŭ·Î´×ÇÏ´Â °æ¿ì Mighty Cloning Reagent Set (Blunt End) (Code 6027)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
°ü·ÃÁ¦Ç°
- Á¤È®µµ ³ôÀº PCR È¿¼ÒÀÇ premix ¹öÀü: PrimeSTAR¢ç GXL Premix
- A-tailing & TA cloning kit: Mighty TA-cloning Reagent Set for PrimeSTAR¢ç
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ ¿ªÀü»ç¹ÝÀÀ kit: PrimeScript¢â II 1st strand cDNA Synthesis Kit
- Gel elution¿ë Agarose: SeaPlaque¢ç Agarose
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â : Clontech PCR Thermal Cycler GP

Keyword : PCRÈ¿¼Ò,Á¤È®µµ,high fidelity,high fidelity È¿¼Ò,Ŭ·Î´×,cloning,ligase,PCR mix,PCR premix

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