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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > Total RNA-Seq Kit (FFPE, LCM)

Total RNA-Seq Kit (FFPE, LCM)

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Clontech
634938
SMARTer¢ç Universal Low Input RNA Kit for Sequencing
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ȸ
(Package)
2,184,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32401, x32402, x96299
Clontech
634940
SMARTer¢ç Universal Low Input RNA Kit for Sequencing
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25 ȸ
(Package)
4,678,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

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SMARTer Universal Low Input RNA Kit for SequencingÀº ³·Àº RIN °ªÀ» °¡Áö´Â ¼Ò·®ÀÇ RNA »Ó¸¸ ¾Æ´Ï¶ó non-polyA mRNA (Bacteria sample)·ÎºÎÅÍ °£´ÜÇÏ°í È¿°úÀûÀ¸·Î RNA-seq¿¡ Àû¿ë °¡´ÉÇÑ cDNA¸¦ ÇÕ¼º °¡´ÉÇÏ´Ù. ÀÌ KitÀº ºÒ°ú 200 pgÀÇ rRNA-depleted RNA (2-10 ngÀÇ total RNA·Î Áغñ)·ÎºÎÅÍ NGS¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â cDNA¸¦ Á¶Á¦ÇÒ ¼ö ÀÖ´Ù. ƯÈ÷ SMARTer Universal Low Input RNA Kit for SequencingÀº FFPE³ª LCM (Laser Captured Materials) »ùÇÿ¡ Àû¿ëÇÒ ¼ö ÀÖµµ·Ï °í¾È µÇ¾î ÀÖÀ¸¸ç ±âÁ¸ÀÇ ¹æ¹ý¿¡ ºñÇØ body coverage, ÀçÇö¼º, mappability ¼º´ÉÀÌ ÁÁ´Ù.


±×¸² 1. NGS Workflow for the SMARTer Universal Low Input RNA Kit for Sequencing.
Features
  • Sensitivity to handle low-input samples-Works with as little as 200 pg of input RNA rRNA-depleted material (derived from 2-10 ng of total RNA)
  • Efficient results from compromised samples-Ideal solution for FFPE samples
  • RNA-seq data quality-Consistent RNA-seq data across all input ranges (200 pg-10 ng)
    • Mappability > 80%
    • Reproducibility >0.98
    • ERCC correlation > 0.99
    • Number of genes > 15,000
  • Complete kit includes RT and PCR enzymes
Applications
  • Generate cDNA for RNA-seq from very low amounts of total RNA (2-10 ng) from FFPE or LCM samples
  • Generate cDNA for NGS from very low amounts of total RNA (2-10 ng) that are:
    • Compromised (obtained from FFPE or LCM samples)
    • Have low RIN values
    • Are from sources that do not have polyadenylated mRNA
RNA-seq Solutions from Clontech

Product

SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing

SMARTer¢ç Universal Low Input RNA Kit

Code

634888

634938

size

12ȸ

10ȸ

Application

* Transcriptome analysis (mRNA-seq) 
* Ultra Low input mRNA-seq 
* Single cell mRNA-seq

* mRNA-seq, Total RNA-seq, Non-coding RNA-seq
* Low input RNA-seq

Input material

* 10pg - 10ng input of mRNA
* High quality total RNA (RIN>8)
* Single cell
* Few cells (1 - 1,000 cells)

* 200pg - 10ng input of rRNA depleted RNA
* Degraded samples (fragmented RNA) (RIN 2-3)
  - FFPE, Laser capture microscopy(LCM)
* Non polyadenylated RNA (Bacteria)

Technology

* Oligo dT Priming
* Works both isolated RNA and directly on cells

* Random priming
* Requires ribosomal RNA removal
* Requires RNA isolation


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Stranded RNA-seq
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