´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
siRNA µµÀÔ ¿ë ½Ã¾à

TransIT-TKO Transfection Reagent

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 2154
TransIT-TKO Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.4 ml
°¡°Ý¹®ÀÇ   ml015_transit_tko_transfection_reagent.pdf
Mirus
MIR 2150
TransIT-TKO Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1.5 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 2155
TransIT-TKO Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.5 ml¡¿5
°¡°Ý¹®ÀÇ  
Mirus
MIR 2156
TransIT-TKO Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.5 ml¡¿10
°¡°Ý¹®ÀÇ  

  • ±¤¹üÀ§ÇÑ ¼¼Æ÷¿¡ siRNA¸¦ °íÈ¿À²·Î µµÀÔ °¡´É
  • ³·Àº ¼¼Æ÷ µ¶¼ºÀ¸·Î ¹èÁö±³È¯ ºÒÇÊ¿ä
  • ³ôÀº Knock-down È¿À²·Î, ÀçÇö¼º ³ôÀº ½ÇÇè °á°ú

Technical Report: High Efficiency siRNA Delivery In Vitro (pdf) (Ŭ¸¯)
Publication: Delivery of small interfering RNA to mammalian cells in culture (Ŭ¸¯)
Á¦Ç°¼³¸í
TransIT-TKO´Â ªÀº dsRNA (siRNA)À» ÁøÇÙ¼¼Æ÷¿¡ transfectionÇϱâ À§Çؼ­ ÃÖÀûÈ­µÈ ½Ã¾àÀÌ´Ù. ¼¼Æ÷¿¡ ´ëÇÑ µ¶¼ºÀÌ ³·°í, ³ôÀº È¿À²·Î siRNA¸¦ µµÀÔÇÒ ¼ö ÀÖÀ¸¹Ç·Î Ç¥Àû À¯ÀüÀÚÀÇ ¹ßÇöÀ» Knock-down ÇÏ´Â ½ÇÇè¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ Ç÷ûÀ» Æ÷ÇÔÇÏ´Â ¹èÁö¿¡µµ transfectionÀÌ °¡´ÉÇϸç, ¹èÁö±³È¯ÀÌ ºÒÇÊ¿äÇϹǷΠ½Ã°£À» Àý¾àÇÒ ¼ö ÀÖ´Ù.
24 well plateÀÇ °æ¿ì º» ½Ã¾à 1 mlÀ¸·Î ¾à 500ȸºÐÀÇ transfectionÀ» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù. ±×¸®°í TransIT-LT1 (Code MIR 2304/MIR 2300)°ú ÇÔ²² ÀÌ¿ëÇϸé siRNA ¿Í plasmid DNAÀÇ co-transfectionÀÌ °¡´ÉÇÏ´Ù.


Figure 1. High Efficiency Knockdown and Low Toxicity Using TransIT-TKO¢ç Reagent in HeLa Cells.  Firefly and sea pansy luciferase reporter vectors were co-transfected into HeLa cells using TransIT¢ç-LT1 Reagent. Cells were incubated for at least 4 hours, split into 24-well plates, allowed to adhere and transfected with 25 nM of either a non-targeting siRNA or an anti-firefly luciferase siRNA using the indicated reagents with the volumes noted beneath each well. Luciferase expression, normalized to non-targeting siRNA control (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.


Figure 2. Delivery of Fluorescently-Labeled siRNA Using TransIT-TKO¢ç Transfection Reagent.  HeLa (70% confluence) cells in 12-well plates were transfected with TransIT-TKO¢ç Transfection Reagent (3 ¥ìl/well) and Label IT¢ç siRNA Tracker Fluorescein-labeled siRNA duplexes (GREEN, 50 nM final concentration in the well). The cells were incubated 24 hours post-transfection then fixed and counterstained with TO-PRO¢ç-3 (nuclei, BLUE) (Life Technologies) and Alexa Fluor¢ç 546 Phalloidin (actin, RED) (Life Technologies). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope.

Ç¥ 1. TransIT-TKO¸¦ ÀÌ¿ëÇÑ ¸ñÀû À¯ÀüÀÚ Knock-down È¿À²
¼¼Æ÷

Target Gene

Knock-Down efficiency
(qRT-PCR)

BNL CL.2 (mouse liver)

MAPK1

80 %

MAPK3

83 %

HeLa (human cervix)

Lamin A / C

80 %

GAPDH

80 %

Hepa1c1c7 (mouse liver)

MAPK1

80 %

MAPK3

75 %

MEK1

75 %

PTEN

80 %

HepG2 (human liver)

MAPK1

80 %

NIH 3T3-L1

MAPK1

70 %

MAPK3

70 %

Secondary Human Astrocytes

Lamin A / C

80 %

Primary Mouse Hepatocytes

ABC A1

70 %

Lamin A / C

81 %

º¸Á¸ 4¡É

Keyword : V2154,MIR2154,siRNA,V2150,MIR2150,V2155,MIR2155,V2156,MIR2156

siRNA Transfection ½Ã¾à
>
µµÀÔ È¿À² È®ÀÎ ¿ë Ç¥½Ä Control
Label IT Delivery Control
>
Mouse »ýü ³» ¼¼Æ÷ ¿ë siRNA µµÀÔ ½Ã¾à
TransIT-QR Hydrodynamic Delivery Solution
>
siRNA µµÀÔ ¿ë ½Ã¾à
TransIT-siQUEST Transfection Reagent
>
siRNA µµÀÔ ¿ë ½Ã¾à
TransIT-TKO Transfection Reagent
>
Plasmid DNA¿Í siRNA/miRNA¸¦ È¿°úÀûÀ¸·Î deliv..
TransIT-X2 Dynamic Delivery System