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Home > ÀüÁ¦Ç°º¸±â > ´ç»ý¹°°øÇÐ > ´ç¼âÇؼ® È¿¼Ò > Cloned Glycopeptidase F£¨Peptide£ºN-glycosidase F£©
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Cloned Glycopeptidase F£¨Peptide£ºN-glycosidase F£©

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
4450
Glycopeptidase F (Peptide:N-glycosidase F)
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 mU
339,000¿ø  4450_DS.v1905Da.pdf

Çü»ó
µ¿°á°ÇÁ¶Ç° [50 mM EDTA¿Í 0.05% sodium azide¸¦ ÇÔÀ¯ÇÏ´Â 20 mM Sodum Phosphate ¿ë¾× (pH7.2)]
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A. Denature buffer : 1% SDS/ 1 M Tris-HCl (pH8.6)
B. Native buffer : 1M Tris-HCl (pH8.6)
C. Stabilizer solution : 5% Nonidet P-40
D. Contorl glycoprotein : 10 mg/ ml Bovine Fetuin
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µ¿°áÀ¶ÇØÀÇ ¹Ýº¹À» ÇÇÇÑ´Ù.
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3. 5. 1. 52
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E.coli carrying the plasmid including Glycopeptidase F gene
¹ÝÀÀ
N-glycosideÇü ´ç¼â¿Í ´Ü¹éÁú°úÀÇ °áÇÕºÎÀ§(GlcNAc-Asn °áÇÕ)¸¦ ƯÀÌÀûÀ¸·Î Àý´ÜÇÑ´Ù.
È°¼ºÀÇ Á¤ÀÇ
37¡É, pH8.6¿¡¼­ dansyl-fetuin glycopeptide·ÎºÎÅÍ 1ºÐµ¿¾È 1 ¥ìmolÀÇdansyl - fetuin deglycosylated peptide ¸¦ »ý¼ºÇÏ´Â È¿¼Ò È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
¿ëÇعæ¹ý
50 §¡ÀÇ ¸ê±Õ¼ö¿¡ ¿ëÇØÇÑ´Ù (500 mU/ ml).
License Notice : [M15, M16]

Keyword :

´ç¼âÇؼ® È¿¼Ò
>
´ç´Ü¹éÁú ¿¬±¸¿¡ Çʼö ½Ã½ºÅÛ
Cloned Glycopeptidase F£¨Peptide£ºN-glycosidase F£©
>
Amino acid °áÇÕÀ» ½Äº°
Cloned ¥á2,3-Sialidase
>
I Çü ´ç¼â¸¦ ½Äº°
Lacto-N-biosidase
>
Fucose °áÇÕÀ» ½Äº°
¥á-1,2-L-Fucosidase
>
Fucose °áÇÕÀ» ½Äº°
¥á-1,3/4-L-Fucosidase