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Lacto-N-biosidase

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
4456
Lacto-N-biosidase
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ¥ìU
679,000¿ø  4456_DS.v1812Da.pdf

³óµµ : 1 ¥ìU/ §¡
Çü»ó
¿ë¾× [0.05% Brij 58À» ÇÔÀ¯ÇÏ´Â 50 mM sodium acetate ¿ÏÃæ¾×(pH5.5)]
º¸Á¸ : 4¡É (1³â°£ ¾ÈÁ¤)
À¯·¡
Streptomyces sp. 142
È¿¼Ò¹øÈ£
3. 2. 1. 140
Á¦Ç°¼³¸í
º» Á¦Ç°Àº 1Çü ´ç¼â±¸Á¶¿¡ ƯÀÌÀûÀ¸·Î ÀÛ¿ëÇÏ¿© ´ç¼âÀÇ ºñȯ¿ø ¸»´ÜÀ¸·ÎºÎÅÍ lacto-N-biose (Gal ¥â1-3GlcNAc)À» À¯¸®Çϳª, 2ÇüÀÇ ´ç¼â¿¡´Â ÀüÇô ÀÛ¿ëÇÏÁö ¾Ê´Â´Ù. ´ç´Ü¹éÁúÀ̳ª ´çÁöÁú ´ç¼âÀÇ 1Çü, 2Çü ±¸Á¶ÀÇ ÆǺ°¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
¶Ç ¥á-1, 3/4-Fucosidase (TaKaRa Code 4453)°ú Á¶ÇÕÇÏ¿© »ç¿ëÇϹǷνá Lewisa ±¸Á¶¿Í LewisX ±¸Á¶¸¦ ½Äº°ÇÒ ¼ö ÀÖ´Ù. ´ç´Ü¹éÁúÀ̳ª ´çÁöÁúÀÇ ´ç¼â ±¸Á¶¿Í ±â´ÉÀÇ Çؼ®¿¡ À¯¿ëÇÏ´Ù. º» È¿¼Ò 100 ¥ìU (1 ¹ÙÀ̾˺Ð)À¸·Î ´ç¼â ~10 pmol¿¡ ´ëÇÏ¿© ¾à 50~100ȸºÐÀÇ È¿¼Ò ¼ÒÈ­°¡ °¡´ÉÇÏ´Ù.
¹ÝÀÀ
ºñȯ¿ø ¸»´Ü¿¡ 1Çü ´ç¼â±¸Á¶ (Gal ¥â1-3GlcNAc-)¸¦ °®´Â ´ç¼â¿¡ ÀÛ¿ëÇÏ¿© lacto-N -biose (Gal ¥â1-3GlcNAc)¸¦ À¯¸®ÇÑ´Ù.
È°¼ºÀÇ Á¤ÀÇ
37¡É, pH5.5¿¡¼­ PA-lacto-N-tetraose·ÎºÎÅÍ 1ºÐµ¿¾È 1 ¥ìmolÀÇ PA-lactose¸¦ »ý¼ºÇÏ´Â È¿¼Ò·®À» 1 U¶ó ÇÑ´Ù.
License Notice : [M15, M16]

Keyword :

´ç¼âÇؼ® È¿¼Ò
>
´ç´Ü¹éÁú ¿¬±¸¿¡ Çʼö ½Ã½ºÅÛ
Cloned Glycopeptidase F£¨Peptide£ºN-glycosidase F£©
>
Amino acid °áÇÕÀ» ½Äº°
Cloned ¥á2,3-Sialidase
>
I Çü ´ç¼â¸¦ ½Äº°
Lacto-N-biosidase
>
Fucose °áÇÕÀ» ½Äº°
¥á-1,2-L-Fucosidase
>
Fucose °áÇÕÀ» ½Äº°
¥á-1,3/4-L-Fucosidase