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Amino acid °áÇÕÀ» ½Äº°

Cloned ¥á2,3-Sialidase

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
4455
¥á2, 3-Sialidase
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 U
502,000¿ø  4455_DS.v1810Da.pdf

Çü»ó
µ¿°á°ÇÁ¶Ç° (0.1 M NaCl°ú 0.02% sodium azide¸¦ ÇÔÀ¯ÇÏ´Â 50 mM Potassium phosphate buffer (pH6.8) 100 §¡¸¦ µ¿°á°ÇÁ¶)
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-20¡É (8°³¿ù°£ ¾ÈÁ¤, ¿ëÇØÇÏ¿© 4¡É¿¡ º¸°üÇÏ´Â °æ¿ì´Â 6°³¿ù°£ ¾ÈÁ¤, µ¿°áÀ¶ÇØÀÇ ¹Ýº¹Àº ÇÇÇÑ´Ù)
È¿¼Ò¹øÈ£
3. 2. 1. 18
À¯·¡
Cloned from Salmonella typhimurium LT2 and expressed in Escherichia coli
¹ÝÀÀ
´ç¼âÁßÀÇ ¥á-2, 3 sialyl °áÇÕÀ» ƯÀÌÀûÀ¸·Î Àý´ÜÇÑ´Ù.
È°¼ºÀÇ Á¤ÀÇ
37¡É, pH5.5¿¡¼­ 3¡¯-sialyllactose·ÎºÎÅÍ 1ºÐµ¿¾È 1 ¥ìmolÀÇ sialic acid¸¦ À¯¸®ÇÏ´Â È¿¼Ò È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.

Keyword :

´ç¼âÇؼ® È¿¼Ò
>
´ç´Ü¹éÁú ¿¬±¸¿¡ Çʼö ½Ã½ºÅÛ
Cloned Glycopeptidase F£¨Peptide£ºN-glycosidase F£©
>
Amino acid °áÇÕÀ» ½Äº°
Cloned ¥á2,3-Sialidase
>
I Çü ´ç¼â¸¦ ½Äº°
Lacto-N-biosidase
>
Fucose °áÇÕÀ» ½Äº°
¥á-1,2-L-Fucosidase
>
Fucose °áÇÕÀ» ½Äº°
¥á-1,3/4-L-Fucosidase